反硝化抑制硫酸盐还原的工艺特性金鹏康

38520175ENVIＲONMENTALSCIENCEVol．38No．5May20171112331．7100552．0140303．710055UASB．NaNO2denitrifyingbacteriaDNB7.0×103CFU·100mL－17.3×105CFU·100mL－1DNBsulfate-reducingbacteriaSＲBSＲB8.0×105CFU·100mL－17.6×104CFU·100mL－182%．1200S2－92%92%．．1.0～1.4mm1.17mm1.2～1.6mm1.21mm47.6m·h－1．PCＲ-DGGE18144362.6%SＲBDNBSＲB42UnculturedSulfurimonassp．SＲBSＲB．SＲBDNBPCＲ-DGGEX703.1A0250-3301201705-1982-09DOI10．13227/j．hjkx．2016110972016-11-112016-12-14509782111974～[e-mail]trificationInhibitingSulfateＲeducingProcessJINPeng-kang1YANGZhen-rui1LIＲong12LIYan3ZHOULi-hui31．SchoolofEnvironmentalandMunicipalEngineeringXi'anUniversityofArchitectureandTechnologyXi'an710055China2．BaotouFinancialInvestmentandAssessmentCenterBaotou014030China3．OilandGasTechnologyＲesearchInstituteChangqingOilFieldCompanyXi'an710055ChinaAbstractInthisstudyasetoftwo-stageUASBreactorwasusedtostudythecharacteristicsofdenitrificationinhibitingsulfatereductioninoilfieldgatheringandtransportationsystemandsomehighsaltwastewaterandthecharacteristicsofgranularsludgeandmicrobialcharacteristicswerestudiedafterthestableoperationoftheprocess．TheresultsshowedthattheadditionofNaNO2increasedthenumberofdenitrifyingbacteriaDNBfrom7.0×103CFU·100mL－1to7.3×105CFU·100mL－1andremainedstableintheprocessfromstarttomature．ThecompetitiveinhibitioncausedbyDNBdecreasedthenumberofSＲBfrom8.0×105CFU·100mL－1to7.6×104CFU·100mL－1．Meanwhilethesulfatereductionwasinhibitedandtheinhibitionrateincreasedandfinallystabilizedto82%．ThestudyonmassratioofmicrobialmasstonitriteeffectingondenitrificationefficiencyshowedthattheinhibitionrateofS2－wasthehighestreaching92%whenthemassratiowas1200．Theinhibitionrateoftheprocesscouldremainatabout92%withgoodstability．Theparticlesizeandsedimentationrateofgranularsludgeweremeasuredtodeterminewhethertheprocessconditionswerefavorablefortheformationofgranularsludge．Thestudyshowedthatthedenitrifyinggranularsludgeformedwasbrownbasicallyellipsoidalandsphericalwithsmoothsurfaceandhighdensity．Beforethedenitrificationinhibitiontheparticlesizeofgranularsludgewas1.0-1.4mmandtheaveragediameterwas1.17mmandafterdenitrificationinhibitiontheparticlesizedistributionwas1.2-1.6mmtheaverageparticlesizewas1.21mmwhichindicatedthatthedenitrificationinhibitionincreasedthesizeofgranularsludge．Theaveragesedimentationvelocityofdenitrifyinggranularsludgewas47.6m·h－1whichrevealedthegoodsettlingperformanceofthegranularsludge．TheresultsofPCＲ-DGGEanalysisshowedthatthedenitrificationinhibitionreducedthenumberofmicrobialspeciesfrom18to14andthenumberofdominantspeciesdecreasedfrom4to3indicatingthedecreasingmicrobialdiversity．Thesimilarityofmicrobialpopulationbeforeandafternitrificationwas62.6%andthepopulationstructurechangedgreatlyfromSＲBtoDNB．ThenumberofdominantSＲBspeciesdecreasedfrom4to2afterdenitrificationinhibitionandtheabundanceofeveryspeciesdecreasedobviously．ThemainfunctionalbacterialspeciesoftheinhibitionprocesswasunculturedSulfurimonassp．whichisakindofautotrophicdenitrifyingbacteria．ItdominatedandcompetedwithSＲBforelectronsinhibitingthesulfatereduction5andtheproductionofsulfide．Keywordsdenitrificationsulfate-reducingbacteriaSＲBdenitrifyingbacteriaDNBgranularsludgePCＲ-DGGEsulfate-reducingbacteriaSＲB．H2SH2S1SＲB2．SＲB3、4～6SＲB7～10．SＲB、denitrifyingbacteriaDNB11DNBSＲB12SＲB13．．14、SＲB＞＞15SO2－4/NO－3SO2－4/NO－38∶1.2Sturman16NO－236h7．、．PCＲ-DGGE．11.11．up-flowanaerobicsludgebedUASB．UASB．UASB-1UASB-2S2－NaNO2UASB-2DNBSＲB．40L．UASB-1HＲT=6.95hUASB-2HＲT=4.49hUASB-2NaNO215.0mL·h－1．17、、、．UASB18L·d－1．1Fig．1Schematicdiagramoftheexperimentaldevice1.21．．3891381/mg·L－1Table1Ｒawwaterquality/mg·L－1COD3009001500NH+46.4319.2932.15TP3.079.2115.35SO2－43009001500pH5.5～7.51.3COD、NH+4、TP、SO2－4、NO－2、S2－18．pHPHS-3pH．SＲB19～217dSＲB．DNBDNB“”142223．KNO32gK2HPO41.0gMgSO4·7H2O0.2gKH2PO41.0g5.0g1000mLpH7.2．1mL3～54～61mL．25～30℃14d．3．SVI24．25．2.4、2.2、2.0、1.8、1.6、1.4、1.2、1.0、0.8、0.6、0.40.2mm105℃、．26．＞1L20～30v=h/tht．DNADNAOMEGASoilDNAkit．PCＲ-DGGEV3338F5'-ACTCCTACGGGAGGCAGC-3'518Ｒ5'-ATTACCGCGGCTGCTGG-3'GC．27．22.1．SO2－4300mg·L－131d900mg·L－161d1500mg·L－1．2UASB-2SO2－4UASB-2SO2－4UASB-1UASB-2．UASB-2SO2－475d93%．3UASB-2CODS2－CODSO2－4S2－102mg·L－1．4UASB-2SＲBUASB-2SＲB2.0×103CFU·100mL－175d8.0×105CFU·100mL－1SＲB．．2UASB-2SO2－4Fig．2ChangeofSO2－4inUASB-2duringstart-up2.22.2.1UASB-2NaNO2SＲB．10SO2－4/NO－38∶1.2NO－2222mg·L－1．222mg·L－1．5、6UASB-2S2－．489153UASB-2Fig．3ChangesofwaterqualityinUASB-2duringstart-up4UASB-2SＲBFig．4ChangeofSＲBquantityinUASB-2duringstart-upDNB．5103mg·L－1162d18.4mg·L－1S2－82%S2－DNBNO－2．6SＲBDNBSＲBDNB．3．SＲB8.0×105CFU·100mL－1DNB7.0×103CFU·100mL－1NO－2SＲBDNBNO－2116dNO－2DNBSＲB146dNO－2162dDNB7.3×105CFU·100mL－1SＲB7.6×104CFU·100mL－1．5UASB-2Fig．5ChangeofsulfideinUASB-2duringtheprocessstabilizing6UASB-2Fig．6ChangeofmicrobialquantityinUASB-2duringtheprocessstabilizing2.2.2．．1667、12008573S2－．2LSVI13.3mL·g－1150g3NO－290、125175mg．250mg·L－1NaNO2NaNO2UASB-2NO－215.0、20.929.2mL·h－1．10dNaNO220.9mL·h－110d29.2589138mL·h－110d．UASB-2S2－7．71667S2－83%181dNaNO21200S2－92%191dNaNO2857S2－92%．3S2－NaNO2DNBNaNO2DNB1200DNBS2－NaNO2DNB．1200S2－．S2－NO－21200．1