废水生物处理出水中溶解性微生物产物的形成机制与特征余萍

:　,,1979,,。*(No.50408026)。废水生物处理出水中溶解性微生物产物的形成机制与特征*余萍1于鑫1戢启宏2齐枝花1魏谷1翟建平1(1,,210093;2,210036)　　　(SMP),,。SMP,。　　SMPTheformationmechanismandcharacteristicsofsolublemicrobialproducts(SMP)inwastewaterbiologicaltreatments　YuPing1,YuXin1,JiQihong2,QiZhihua1,WeiGu,ZhaiJianping1.(1.StateKeyLaboratoryofPollutionControlandResourceReuse,SchooloftheEnvironment,NanjingUniversity,NanjingJiangsu210093;2.Envi-ronmentMonitoringCenterofJiangsuProvince,NanjingJiangsu210036)Abstract:　Theexistenceofsolublemicrobialproducts(SMP)producedbymicrobialculturesinvolvedinbiolog-icalwastewatertreatmentprocesshasbeenwidelyinvestigated.SMPs,whichwerereleasedfromsubstratemetabo-lismandmicrobialendogenousgrowth,arefoundtobeanimportantfactorwhichaffectsthequalityofthebiologicallytreatedeffluentandthedegradationoforganiccompounds.Areviewconcerningthedefinition,characteristicsofSMPanditsformationmechanismispresentedinthepaper.Keywords:　Biologicaltreatment　Solublemicrobialproducts(SMP)　Chelating　Toxicity　Biodegradability　Molecularweight(MW)distribution　　,,,。,,,,(SolubleMicrobialProducts,SMP)。SMP,、、。[1,2],、,SMPCOD100%,,。、,SMPCOD。,SMP。　　Namkung[3]SMP(Substrate-utilization-associatedProducts,UAP)(Biomass-associatedProd-ucts,BAP)。UAP、,、,。BAP,,,、,。SMP,,;SMP,,;SMP,,,,。1　SMP　　(),[4]。,352　　28　5　20065DOI:牨牥牨牭牴牳牭牤jcnki牨牥牥牨牫牳牰牭牪牥牥牰牥牭牥牨牨,SMP。,SMP(SMPSD),[5]。SMP,3:;;。1.1　与基质利用和微生物生长相关型　　,,SMP,(1)。SMP,SMPSDSMP[6],(2)。,,SMPSD。SMP,SMP,UAP,。(S0)+→+UAP+CO2(1)SMPSD+→+UAP’+CO2(2)　　SMP,SMP。,。[7]。1.2与微生物衰亡相关型　　,,,SMP,BAP,(3)。,,,,,,。,,DNA,[8]。Aquino[9],,,,SMP95%45%,(SMP)3%37%,SMP。DNA,SMP。→BAP+CO2(3)　　3:、[4]。,,,,(Yobs)(Y)。。,。()。,,,。。,,。1.3　与环境相关型　　SMP,SMP[10]。,,SMP,。(EPS)SMP[11,12]。、、、、EPS。,EPS,。AquinoStuckey[13]()()SMP。,(VFAs),SMP,SMP。[9]。,SMP2%8%,SMP/S020%。SMPBAP[11]。2　SMP2.1　分子量分布　　SMP,。,,、、、、、。。UAPBAP,,UAP353　　,BAP,。　　SMP,,SMP[14]。1[14],SMP1000,10000,,100010000。1　SMP(MW)MW10001000MW10000MW10000SRT=15d(43±7.8)%(9.8±8.6)%(48±12.2)%SRT=25d(27±6.9)%(11±5.0)%(62±7.0)%SRT=40d(16±8.6)%(8.4±3.0)%(76±9.0)%2　NiMWlogβ2mgCOD/mgmol/mgCOD6011.401.071.56×10-57420.851.511.79×10-58820.731.821.25×10-5121210.701.191.39×10-57536.200.646.25×10-519216.700.756.94×10-616613.001.464.13×10-67632.300.636.27×10-514726.500.981.39×10-510424.100.623.10×10-5ECP1)--5.49-1.6×10-8SMP2)-13.62±0.02-(2.49±1.28)×10-6　　:1)Klebsiellaaerogenes;2)。2.2　螯合功能　　SMP。SMP,。SMP、、、,、、、、,(Zn、Cu、Fe、Pb、Ni)。Kuo[14]SMPNi,402mg/L()1mmol/LNi,SMP。2[14]Ni。2,SMP,,,ECP。2.3　可生物降解性　　,SMP,。Gaudy[15]SMP,90%COD。(ABR)65%～82%[16]。1[5]SMPSD,:(SOC)25%3%3%1%,SMP。1　I、II,TOC　　1I、II,TOC[5]。SMP,。,[17]。UAPBAP[18]。2.4　毒性及抑制作用　　,,[8,19],,SMP354　　28　5　20065。SMP(—OH、—SH—NH2),,。SMP[20,21]。2[20],(μg/mg,SSTTC)TOC,TOC,。,SMPAmes[22];,SMP,NH3-N[2,23]。2　TOC3　　　SMP,SMP,SMP。SMP,:　　(1),SMP。,SMP。SMP,,,,,。　　(2)SMP,,。SMP。　　(3)SMP,SMP、。　　(4)SMP,。,,SMP。1　GooranyO,OztürkI.Solublemicrobialproductformationduringbiologicaltreatmentoffermentationindustryeffluent.Wat.Sci.Technol.,2000,42(1～2):111～1162　,,,.—.:2002,3(1):1～73　NamkungE,RittmannBE.Solublemicrobialproducts(SMP)formationkineticsbybiofilms.Wat.Res.,1986,20(6):795～8064　GradyJrCPR,DaiggerGT,LimHC.,..:,20035　BoeroVJ,BowersSR,EckenfelderWWJr.Molecularweightdistributionofsolublemicrobialprodudtsinbiologicalsystems.Wat.Sci.Technol.,1996,34(5～6):241～2486　LaspidouCS,RittmannBE.Amodelformembranebioreactorprocessbasedontheconceptofformationanddegradationofsol-ublemicrobialproducts.Wat.Res.,2001,35(8):2038～20487　KindaichiT,ItoT,OkabeS.Ecophysiologicalinteractionbetweennitrifyingbacteriaandheterotrophicbacteriainautotrophicnitrif-yingbiofilmsasdeterminedbymicroautoradiography-fluores-cenceinsituhybridization.Applied&EnvironmentalMicrobiolo-gy,2004,70(3):1641～16508　..:,20019　AquinoSF,StuckeyDC.Productionofsolublemicrobialproducts(SMP)inanaerobicchemostatsundernutrientdeficiency.JournalofEnvironmentalEngingeering,2003,129(11):1007～101410　ChudobaJ.QuantitativeestimationinCODunitsofrefractoryorganiccompoundsproducedbyactivatedsludgemicroorgan-isms.Wat.Res.,1985,19(1):37～4311　BarkerDJ,StuckeyDC.Areviewofsolublemicrobialproducts(SMP)inwastewatertreatmentsystems.Wat.Res.,1999,33(14):3063～308212　LaspidouCS,RittmannBE.Aunifiedtheoryforextracellularpolymericsubstances,solublemicrobialproducts,andactiveandinertbiomass.Wat.Res.,2002,36(11):2711～272013　AquinoSF,StuckeyDC.SolubleMicrobialProductsformationinanaerobicchemostatsinthepresenceoftoxiccompounds.Wat.Res.,2004,38:255～26614　KuoWC,ParkinGF.Characterizationofsolublemicrobialproductsfromanaerobictreatmentbymolecularweightdistri-butionandnickel-chelatingproperties.Wat.Res.,1996,30(4):915～92215　GaudyAFJr,BlachlyJR.Astudyofthebiodegradabilityofre-sidualCOD.Proc.39thPurdueindustrialwasteconf.Indiana:W.Lafayette,198416　BarkerDJ,StuckeyDC.Modelingofsolublemicrobialproductsinanaerobicdigestion:Theeffectoffeedstrengthandcomposi-tion.Wat.Environ.Res.,2001,73(2):173～18417　BarkerDJ,MannucchiGA,SalviSML,etal.Characterisationofsol-ubleresidualchemicaloxygendemand(COD)inanaerobicwastewatertreatmenteffluents.Wat.Res.,1999,33(11):2499～251018　UrbainV,MobarryB,DeSilvaV,etal.Integrationofperf