废水生物膜动力学参数的研究方法

2008,28(8)679~682ChinaEnvironmentalScience,,*(,,100084),.,,,,.qmax=10mg(O2)/[g(VSS)⋅h],KO=1.2mg/L.,qmax,kO.X703A10006923(2008)08067904Anapproachtostudybio-kineticparametersinwastewaterbiofilms.QIUYu-qin,ZHOUXiao-hong,SHIHan-chang*(StateKeyJointLaboratoryofEnvironmentalSimulationandPollutionControl,DepartmentofEnvironmentalScienceandEngineering,TsinghuaUniversity,Beijing100084,China).ChinaEnvironmentalScience,2008,28(8)679~682AbstractWhenadequatesubstratewasprovidedtobiofilm,theoxygenconcentrationinthebiofilmbecametheonlylimitedfactortothebiofilmgrowth.underthiscondition,aseparatedoxygenmicroelectrodewasusedasdetectingtooltodetecttheoxygendistributioninthebiofilm.Basedonthediffusion-reactionequationunderthestabilizatingcondition,biofilmkineticparameterswerecalculatedbythemethodofhuntingstepbystep.Theresultsofthebiofilmkineticparameterscalculatedwereqmax=10mgO2/(gVSS⋅h),KO=1.2mg/L.Comparedwiththeactivatedsladgesystem,theqmaxwassmallerandthekOwashigher.Keywordsbiofilmkineticparametermicroelectroddiffusion-reaction,.2:[12],,,.[34],.,.Yurt[5]LeptothrixdiscophoraSP6,.,,,,.11.1.500mL,..(3cm×3cm).,COD400mg/L,12h.(18±2).30d1.5mm,.20071212(50478009);973(2005CB724901);(20040003040)*,,[e-mail]680281.2[6].[7].(WN103TA100M,)(PA2000,Unisense,)(60mm×60mm×10mm)(UA303,)PC.,.,50µm,,.1.3,:2effmaxf2Od()dCCD=qXK+Cx(1),maxmax/,qYµ=2:x=0,C=Cb;x=xL,dC/dx=0:Deff,mm2/h;C,mg/L;x,mm;KO,mg/L;Xf,mg/L;maxµ,h1;Y;Cb,mg/L;xL,mm.,,(25µm),,,.,,x1.,,x2,x2x1.,.Cx,[8]Xf.(1)2,qmaxKO,,Matlab.:Cx;[910],qmaxKO0~100mg(O2)/[g(VSS)·h],0~2mg/L.:∆qmax=10mgO2/[g(VSS)⋅h];∆KO=0.1mg/L.bvp4c,(2)(SSD):n2,,1SSD()iiiCC=−∑(2):Ci,,mg/L;Ci,,mg/L.qmax,KOSSD,SSDqmax,KO.22.10123450.60.40.200.20.40.60.811.2(mm)(mg/L)(a)(b)1Fig.1Measuredandsimulatedoxygendistributionsinbiofilms1.5mm,.2000mg/L,4.93mg/L,18.50µm.1(a).2000mg/L,8681.0.25mm,1mg/L.0.70mm,0.1.3mm,VSS0.032g,27.1g/L.[11],5.148mm2/h.2.2Matlab,qmax=10mg(O2)/[g(VSS)·h],KO=1.2mg/L.,1(b),SSD0.2889,.qmax.,qmaxd1[10mgO2/(gVSS⋅h)0.17d1].1(ASM1),IWA[12]6.00d1,0.67,8.96d1.,.IWAASM1.,,,,.Plattes[13],1.57d1,,,,[14].,.KO,.KO.KS,.,.ASM120mg/L,.,,,.,2007,Brockmann[15],,,2.0mg/L.,,.IWA[12]0.2mg/L.,,.33.1,.,,qmax=10mg(O2)/[g(VSS)·h],KO=1.2mg/L.3.2IWA,,.,,.3.3.[1]EkerS,KargiF.Kineticmodelingandparameterestimationinbiologicaltreatmentof2,4-dichlorophenolcontainingwastewaterusingrotatingperforatedtubesbiofilmreactor[J].EnzymeandMicrobialTechnology,2006,38:860866.[2]KreftJU,WimpennyJWT.EffectofEPSonbiofilmstructureandfunctionasrevealedbyanindividual-basedmodelofbiofilmgrowth[J].WaterScienceandTechnology,2001,43(6):135141.[3]ZengH,ZhangTC.Evaluationofkineticparametersofasulfur-limestoneautotrophicdenitricationbiofilmprocess[J].WaterResearch,2005,39(20):49414952.[4]RieflerRG,SmetsBF.Comparisonofatypecurveandaleast-squarederrorsmethedtoestimatebiofilmkineticparameters[J].WaterReasearch,2003,37(13):32793285.[5]YurtN,BeyenalH,SearsJ,etal.Quantifyingselectedgrowth68228parametersofLeptothrixdiscophoraSP-6inbiofilmsfromoxygenconcentrationprofiles[J].ChemicalEngineeringScience,2003,58:45574566.[6],,.[J].,2007,28(3):598602.[7]JensenK,RevsbechNP,MielsenLP.Microscaledistributionofnitrificationactivityinsedimentdeterminedwithashieldedmicrosensorfornitrate[J].AppliedandEnvironmentalMicrobio-logy,1993,59(10):32873296.[8].[M].:,1989.[9]JørgensenPE,EriksenT,JensenBK.EstimationofviablebiomassinwastewaterandactivatedsludgebydeterminationofATP,oxygenutilizationrateandFODhydrolysis[J].WaterResearch,1992,26(11):14951501.[10]ChuKH,vanVeldhuizenHM,vanLoosdrechtMCM.Respirometricmeasurementofkineticparameters:effectofactivatedsludgeflocsize[J].WaterScienceandTechnology,2003,48(8):6168.[11]BeyenalH,TanyolacA.Thecalculationofsimultaneouseffectivediffusioncoefficientsofthesubstratesinafluidizedbedbiofilmreactor[J].WaterScienceandTechnology,1994,29(10/11):463470.[12]HenzeM.GradyCPL.GujerW.ActivatedsludgemodelNo.1IAWPRCtaskgrouponmathematicalmodellingfordesignandoperationofbiologicalwastewatertreatment[M].ScientificandTechnicalReport,London:IAWPRC,1987.[13]PlattesM,FiorelliD.Modellinganddynamicsimulationofamovingbedbioreactorusingrespirometryfortheestimationofkineticparameters[J].BiochemicalEngineeringJournal,2006,33:253259.[14]RieflerRG,AhlfeldDP.Respirometricassayforbiofilmkineticsestimation:Parameteridentifiabilityandretrievability[J].BiotechnologyandBioengineering,1998,57(1):12321239.[15]BrockmannD,MorgenrothE.Estimationofkineticparametersofamodelfordeammonificationinbiofilmsandevaluationofthemodel[J].WaterScienceandTechnology,2007,55(8/9):291299.(1984),,,,....,().;.,.,,.,..:;;;,.,,,.WaterEnvironmentResearchApril,291(2008)