废水中苯胺的好氧共代谢降解实验研究李剑

1　6环境工程学报Vol.1,No.620076ChineseJournalofEnvironmentalEngineeringJun.2007　　　李　剑　　　　　　　　　　　　谢春娟　　(,200072)(,730070)　　。。,,32℃、、1∶6、72h,75.6%。,82.9%,COD55.4%。　　　　　　X703　　　A　　　1673-9108(2007)06-0051-05Studyonaerobicco-metabolismbiodegradationofanilineinwastewaterLiJian(ResearchInstituteofEnvironmentPollutionandHealth,SchoolofEnvironmentalandChemicalEngineering,ShanghaiUniversity,Shanghai200072)XieChunjuan(CollegeofEnvironmentalandMunicipalEngineering,LanzhouJiaotongUniversity,Lanzhou730070)Abstract　Themicroorganismco-metabolismisanimportantmodeofbiodegradationthatcanmetabolizethedifficult-to-degradateorganicmattersinwastewater.Thisstudycomparedthedegradationprocessofanilineassolesourceofcarbonandenergyandco-metabolismdegradationprocessofaniline.Theexperimentalresultre-vealsthatthedegradationratioofanilinecanbeenhancedmarkedlyintheco-metabolismprocesses.Thedegra-dationrateofanilinecanreachat75.6%after72hoursunderthefollowingconditions:constanttemperatureof32℃,usingglucoseasgrowthmatrix,anditsmassproportionwithanilineis1∶6.Afterusingpeptoneasthesourceofnitrogen,thedegradationrateofanilineandtheremovalrateofCODreach82.9%and55.4%respec-tively.Keywords　aniline;aerobicco-metabolism;sourceofcarbon;sourceofnitrogen;biodegradation:2006-08-13;:2006-11-06:(1980～),,,。E-mail:[e-mail]　　。、、,EPA129[1]。,,。,[2]。,,。,,,。LeadbetterFoster1959,,P.methani-ca、,[3]。,,,。1　1.1　:、。3,3A、B、C。1:,20mg/L,NaClKH2PO40.5mg/L,NaOHpH6.0。:THZ-82、HH.S21-6、UV-7500、VIS-723G、80-1。1.2　1.2.1　菌群驯化,:250mL,1g(),100mL,pH6.0,,:30℃,:130r/min,:3d。,,1～2,。,,。:2mL,3d。。1.2.2　测定方法[5]:660nm(OD660nm)()。:2mL3000r/min80-130min,pH6.0UV-7500230nm[4],。2　2.1　:3,。3d20mg/L(0.4mL,0.2mL),:pH6.0,30℃,130r/min,1。1,A、B,1,,。C(10d)A、B,27d1　Fig.1　Experimentonacclimationofmicroorganism,。,CA、B,C,C(36d)WN-12。WN-12:WN-122mL,30℃、130r/min,,6h,OD(WN-12),。2。2　WN-12Fig.2　GrowthcurvesandbiodegradationofanilinebyWN-124:、、。2WN-1230h,,10%。30～42h,,30%,。,,。,WN-1230～42h。526　:2.2　WN-12　　WN-122mL5100mL20mg/L,420mg/L3d,2mL,3～6,:。5,6h,。1,3。1　Table1　Experimentofco-metabolismwithdifferentsourcesofcarbon12345(mL)0.50.50.50.5::30℃,:130r/min,pH:6.03　CFig.3　Effectsofdifferentsourcesofcarbononco-metabolism　　3,WN-12,。WN-12,,、、。,,,。,,,,,0～18h,。2:,WN-1230～42h,3,,18～36h,,,,,,。,,,[2]。,,,:30℃,72h,130r/min,pH6.0(:),4。4　Fig.4　Effectsofdifferentproportionsofglucoseonco-metabolism:。4,,1∶6,,75.6%。,,,,。1∶6,,,。,1∶6。2.3　WN-12WN-12531,,,2,5。2　Table2　Experimentofco-metabolismatdifferenttemperatures(℃)(h)120721∶6224721∶6326721∶6428721∶6530721∶6632721∶6734721∶6836721∶6938721∶61040721∶61145721∶61250721∶65,20～50℃,WN-12,,。,,;,,,。,532℃。5　Fig.5　Effectsofdifferenttemperaturesonco-metabolism2.4　WN-12,。,,,,,。WN-12,,20mg/L。72h,,6,7。6　WN-12Fig.6　Effectofpeptoneonco-metabolismdegradationofanilinebyWN-126,1、2、3、、。61,,、,,WN-12,2。3,,。,WN-12,(30h),。7　CODFig.7　RemovalofCODinanilinewastewater7,COD6,(55.4%),82.9%。,,,,WN-12,,,COD546　:。3　　(1),WN-12,72h37.5%,30～42h。(2)、、、,,WN-12。,72h65%。(3),1∶6,WN-1275.6%。(4)WN-12,,32℃。(5),WN-12,(30h),。72h82.9%,COD55.4%,,。[1]KeithL.H.,TelliardW.A.PrioritypollutantsI.Aper-spectiveview.Environ.Sci.Technol.,1979,13:416～423[2]JankeD.,FritscheW.Natureandsignificanceofmicrobialcometabolismofxenobiotics.J.Basic.Microbiol.,1985,25:603～619[3]LeadbetterE.R.,FosterJ.W.Oxidationproductsformedfromgaseousalkanebythebacteriumpseudomonasmethar-ica.Arch.Biochem.Biophys.,1959,82:491～492[4]LyonsC.D.,KatzS.,BarthaR.Mechanismsandpath-waysofanilineeliminationfromaquaticenvironments.Ap-pl.Environ.Microbiol.,1984,48(3):491～496[5]..:,1986.100～12655