分光光度法测定药厂废水中对硝基酚的含量刘国杰

271渤海大学学报(自然科学版)Vol.27No.120063JournalofBohaiUniversity(NaturalScienceEdition)Mar.2006分光光度法测定药厂废水中对硝基酚的含量刘国杰1,邓桂春2,张渝阳3,臧树良4(1.,110001;2,3,4.,110036)　:针对药厂废水特性,利用对硝基酚在苛性碱存在条件下,生成黄色硝基酚负离子化合物的显色反应,测定了药厂废水中对硝基酚的含量。探索了反应的最佳显色条件,以及废水中可能存在的有机及无机物质的干扰情况,为药厂废水中对硝基酚含量的测定提供了方便快速、灵敏度高、重现性好的检测方法。:对硝基酚;药厂废水;分光光度法:O65　　　:A　　　:1673-0569(2006)01-0014-04　　,,(),。,。:、;、,。,、,。,[1]、[2]、。,,,;,4-;[3],610nm,,;[4],360nm,0.1mg/L。,,,,,、。1　实验部分1.1　(722,);UV/VISSprctrometerLambdaBio20(PE);(pHS-3C,);(CS50,);()。(PNP)(AR),(AR)。1.2　1.2.1　标准样品的制备(PNP):1mg/mL,,1μg/mL。1.2.2　试验方法25ml,,,pH10～11,5min,60min,400nm,1cm,,。:2005-09-12.:(1979-),,,.1.2.3　水样测定,,,。,NaOH,,。100,1.0mL100mL。1.0mL25mL,;,,。2　结果与讨论2.1　,,c-c*,318nm(pH=3);,-NO2,c-c*,n-c*,π,,,。,1,400nm。400nm。图1　吸收光谱a.对硝基酚ρ-nitrophenolb.对硝基酚废水wastewater2.2　2.2.1　pH值的影响(PNP),,pH,,2。图2　酸度对显色反应的影响,pH9～14,。pH10～11。2.2.2　显色反应时间的影响,1。1　t/minPNP/A/A30.7310.18750.8060.212100.8060.213200.8070.213300.8070.213600.8100.2171200.8140.22124h0.8250.231　　,5-60min,5min,60min。2.2.3　温度对测定的影响,,2。2　T/℃PNP/A/A50.6710.143100.8100.212200.8110.213300.8110.214400.8160.222500.8210.225,10～30℃,。15　1刘国杰,邓桂春,张渝阳,臧树良:分光光度法测定药厂废水中对硝基酚的含量2.2.4　工作曲线绘制。图3　工作曲线,:A=5.444Cmg/25mL-0.00336,r=0.9999,X=1.3×104L/mol·cm,0.032mg/L,0.62μg/25mL-190μg/25mL。2.2.5　精密度试验,,3,0.25%。3　/APNP/mg/25mLRSD/%10.340.0630720.3390.0628930.3380.0627040.3390.062890.2550.3380.0627060.3390.0628970.340.0630780.340.063072.2.6　干扰试验(PNP),,,±5%,()4。4　RE/%NaCl6000000SO2-46000001.63Zn2+504.83Cu2+124.22Mg2+102.26Fe3+34.73Co2+204.87Ca2+1923.98Ni2+204.57Cr3+144.69NO-310001.731004.91　　PNP,,。2.2.7　样品测定及回收率试验5。5　mg·L-1RSD/%mg/25mlmg/25ml/%/%4360.030.031480.03160100.384378.400.062340.06245101.184323.290.094300.09460100.324341.660.11760.117099.490.42100.343　结论,,、、、,,。参考文献:[1],.[M].:,1990:307-308.[2].[M].:,2002:321-333.[3],.[J].,1994,6(3):34.[4],　.[J].,1998,18(5):44.16渤海大学学报(自然科学版)27DeterminationofnitrophenolinindustrialwastewaterbyspectrophotometryLIUGuo-jie1,DENGGui-chun2,ZHANGYu-yang3,ZANGShu-liang4(1.DepartmentofChemistry,ChinaMedicalUniversity,Shenyang110001,China;2,3,4.DepartmentofChemistry,LiaoningUniversity,Shenyang110036,China)Abstract:Accordingtothecharacteristicofuntreatedwastewaterfrompharmaceuticalfactorythatproducesp-nitrophenol,amethodofdeterminingp-nitrophenolinthiskindofwastewaterbyspectrophotometerisdescribedinthispaper.Theeffectofexperimentalconditionsontheabsorbanceisstudied.Themethodisprovedtobesimpleandquick,andhasheensatisfactorilyappliedtothedeterminationofnitrophenolinwastewater.Keywords:p-Nitrophenol;wastewaterfromharmaceuticalfactory;spectrophotometry17　1刘国杰,邓桂春,张渝阳,臧树良:分光光度法测定药厂废水中对硝基酚的含量