--Vol.29,No.620116ChinaResourcesComprehensiveUtilization,(,510320):、,NH3-N。,、,28~30℃,30‰,5.5mg/L,30L/h120L/h,9d13d,。:;;:X835:B:1008-9500(2011)06-0028-03OptimizationofBiofilmCultureinClosedRecirculatingMaricultureSystemLiXiaoli,WangYujun(GuangdongOceanographicEngineeringVocationalandTechnologySchool,Guangzhou510320,China)Abstract:Tooptimizetheconditionsofbiofilmcultureinclosedrecirculatingmariculturesystem,weinvestigatedfactorsofthenitrificationbacteriaspecies,filtermedias,andgrowthparameters,suchastemperature,salinity,DO,flowrate,ontheremovalofNH3-N.Theresultsdemonstratedthattheoptimalcolonizationofbiofilmwasobservedinsystermusingplasticbio-ballswithbacteriasolutionofgoodnitrificationactivity.Theoperationprocessoftemperaturebetween28℃and30℃,salinity30‰,dissolvedoxygen5.5mg/L,withflowrateincreasedfrom30L/hto120L/h,andstaticanddynamicculturationfor9,and13daysrespectively,reslutedinsuccessfuldemostication.Keywords:closedrecirculatingmariculturesystem;biofilm;culture,[1]。,,,,,,。11.11。、、、、5。PVC,20cm,140cm,,,,。11.2:、、()、。、、NH3-N()AR。1.3[2](1)1gNaNO2、0.03gMgSO4·7H2O、0.25gNaH2PO4、1gNa2CO3、0.75gK2HPO4、0.01:2011-03-25:(1972-),,,,,:。28--gMnSO4·4H2O1000mL(pH7.8),0.1Mpa20min,;,15g。(2)2g(NH4)2SO4、0.03gMgSO4·7H2O、0.25gNaH2PO4、5gCaCO3、0.75gK2HPO4、0.01gMnSO4·4H2O、1000mL,0.1Mpa20min,;,15g。(3)35g1000mL,120℃20min。。22.1、;;NH3-N。2.1、、、,、。(MPN3)。2.2:、1∶1,28℃、127r/min,6d。:1∶9,2d1g(NH4)2SO4、1gNaNO2,,5.5mg/L,9d。MPN3,3,。2.33(、、)9d,3、。2.4,,28~30℃,30‰,5.5mg/L,,30L/h120L/h,13d。、NH3-N。。33.1、1。1(MPN3)1,。28℃14d,,。。3.22。23.39d、、3,MPN3、;,3。3,、,,104/cm3,,。。/mL/mL/cm311112511803103.5×1064.5×1063.5×1064.5×1065.6×1075.9×1072.5×1083.5×1081.8×1062.2×1061.5×1041.0×1051.4×1053.4×1041.6×1051.7×1053.1×1046:29--,。、,、DVD9。,。,。,9,8500()。2000(150)、75(40)。()3.413d,,,。NH3-N4。13d,4.12mg/L0.08mg/L,NH3-N98%。NH3-N0.18kg/(m3·d),,NH3-N0.1~1.5kg/(m3·d)[3],NH3-N,。,。4NH3-N4,9d、13d,。[4]“”(26℃,6mg/L),9.4mg/L,54d0.05mg/L。[5]20℃,pH8,25。,。:(1),。(2),。,、,,;,。,,,。(3),、、、(、、、),,,。28~30℃,pH7.8,。(4),,,5.5mg/L,。1,,,.[J].,2004,24(3):350-354.2.[M].:,2004.3,.[M].:,2005:50-60.4,,,.[J].,2001,20(6):443-445.5,,,.[J].,2003,18(2):84-89.(/)mg/L(d)NH3-N(d)NH3-N14.1282.4525.0191.3138.53100.95410.19110.51510.56120.1567.35130.0874.23630