复合垂直流构建湿地中生物膜的空间分布特性和降解性能吴振斌

512　20054()J.WuhanUniv.(Nat.Sci.Ed.)Vol.51No.2　Apr.2005,204～208　　:2004-12-22　　　　:“”(2002AA601021);(39925007);(2002A00010):(1956-),,,.:1671-8836(2005)02-0204-05复合垂直流构建湿地中生物膜的空间分布、特性和降解性能吴振斌1,李　今1,2,成水平1,付贵萍1,贺　锋1(1.中国科学院水生生物研究所,淡水生态和生物技术国家重点实验室,湖北武汉430072;2.中国科学院研究生院,北京100039)　　　:,、、、、.,,0.0218.,、、,.,0～10cm,、,.,1070,5.1.5.　　:;;;:X171　　　:A0　　　　20.、[1].,、.[2],[3],[4].,.,,.、,;、[5].,,[6].[7,8],[9～11],.、,,.1　1.1　-(integratedvertical-flowconstructedwetland,IVCW)[12].、,9m×9m.,,.,60cm,10cm.DOI:牨牥牨牬牨牳牳牤j牨牰牱牨牳牳牫牰牪牥牥牭牥牪牥牨牰2:、(Cannageneralis),(Acoruscalamus).20047.,IVCW、(60cm)10cm,.(76.2mm×25.4mm×0.8mm),(IVCW),30cm,10.,,.1.2　,105℃,300℃2h,650℃0.5h,,(VSS),[13].　(TTC)[14].:2.00g,37℃12h,,485nm,.　WTW,10g,1.0g,20℃5d,CO2,.　10g,100mL,,,TOC(JenaMultiN/C1000)(TOC),TOC.1.3　,IVCW30cm,.10,,,,100mL,BOD5,、FeCl2、CaCl2、MgSO4,.120r/min28℃,3JenaTOCTOC.,.1.4　,170℃,20.00g;IVCW10cm20.00g;,100mL22mg/L(PCP),,120r/min28℃.PCP.Agilent1100HPLC.2　2.1　IVCW,VSS,.VSS1.1　IVCW　　,0～10cm,VSS2～310cm.,;,.,,.[15].IVCWVSS,,(W)0.0233,VSS0.0202,VSS,,.,[15]..,0.065[16],IVCWVSS0.0218,,.2.2　IVCW2.2.1　,.,.,205()51,.2.2　IVCW　　.,0～10cm,.OD4850.427,OD4850.700,.2.2.2　,3.　　0～l0cm,,1.85,.3　IVCW　　IVCW,.1.0gCO211.2mg/L,VSS3.5%.10gCO27.0mg/L,10%.,5.,,5,IVCW3.0～10cm13.5mg/L,6.409mg/L,2.,.,..,,,,,.2.2.3　IVCW,,TOC,62.7%(4),.,,26.93%、6.19%、11.33%、8.25%、9.96%,.4　IVCWTOC2.3　,,.IVCW5.　　,.1070,2062:、5　,6.78%、7.43%、23.51%、38.21%、40.86%.,1070,5.,.,.(PCP),.IVCWPCP6.6　IVCWPCP　　IVCWPCP,.,71.2%,,83.5%.,,PCP.,PCP51%,IVCW77.3%,1.52,PCP26.3%.IVCWPCP.IVCW.,、、..,.、,.,.3　　,.,.,..,,,.,.,.,.:[1]　HammarDA,BreenPF,PerdomoS,etal.Construc-tedWetlandsforWasteWaterTreatment[M].Michi-gan:LewisPublishersInc,1989.5-20.[2]　RaoTS,RogersKH.BiofilmFormationinaFreshWaterEnvironmentUnderPhoticandAphoticCondi-tions[J].Biofouling,1997,11(4):265-282.[3]　HeadleyJV,MorrisM,TyrellWR,etal.RatesofSorptionandPartitionofContaminantsinRiverBio-film[J].EnvironSciTech,1998,32(24):3968-3973.[4]　SchorerM,EiseleM.AccumulationofInorganicandOrganicPollutantsbyBiofilmsintheAquaticEnviron-ment[J].WaterAirSoilPollut,1997,99:651-659.[5]　CominFA.NitrogenRemovalandCyclinginRestoredWetlandsUsedasFiltersofNutrientsforAgriculturalRunoff[J].WaterSciTech,1997,35(5):255-261.[6]　MantoviP,MitschWJ.ApplicationofaHorizontalSubsurfaceFlowConstructedWetlandonTreatmentofDairyParlorWasteWater[J].BioresourceTechnol,2003,88(2):85-94.[7]　VrhoveskD,FeldeKV,MckinlayRG,etal.Con-207()51structedWetlandforIndustrialWasteWaterTreat-ment[J].WaterRes,1996,30(10):2287-2292.[8]　GerbqCP,GreenM.OptimizationofArtificialWet-landDesignforRemovalofIndicatorMicroorganismsandPathogenicProtozoa[J].WaterSciTech,1999,40(45):363-368.[9]　DrizoA,ReddyKR.Physico-ChemicalScreeningofPhosphateRemovingSubstratesforUseinConstruc-tedWetlandSystems[J].WaterRes,1999,33(7):3595-3602.[10]ToddL,RichardsonCJ,SeidelK,etal.NitrateRe-movalinWetlandMicrocosms[J].WaterRes,1998,32(3):677-684.[11]MichaelL,GellerG,GullyJR,etal.TransformationsinDissolvedOrganicCarbonThroughConstructedWetlands[J].WaterRes,2000,34(6):1897-1911.[12],,　,.[J].,2002,13(6):715-718.WuZhen-bin,ChengShui-ping,HeFeng,etal.DesignandPurificationPerformanceofVerticalFlowCon-structedWetland[J].ChineseJournalofAppliedE-cology,2002,13(6):715-718(Ch).[13]　,,.[M].,,2000.100.LiuYu,ZhaoQing-liang,ZhenXing-chan.BiofilmTechnologyforWastewaterTreatment[M].Beijing:ChinaArchitecture&BuildingPress,2000.100(Ch).[14],,.[M].:,1990.163-165.YuLiu-xin,WuGuo-qing,MengXian-ting.Hand-bookofMicrobiologicalTestsinEnvironmentEngi-neering[M].Beijing:ChinaEnvironmentalSciencePress,1990,163-165(Ch).[15],.、Ⅱ:[J].,1998,10(1):62-66.ChenShui-ping,XiaYi-cheng.ArtificialWetlandwithCattail(Typhaangustifilia),Rush(Juncusefusus),Ⅱ:PurifyingSpace[J].JournalofLakeSciences,1998,10(1):62-66(Ch).[16],.[J].,2000,22(1):4-7.ZhaoQing-liang,HuangRu-chang.BiofilmCharacter-isticsinHybridBiologicalReactor[J].EnvironmentalPollution&Control,2000,22(1):4-7(Ch).SpacialDistribution,CharacteristicsandDegradationCapabilityoftheBiofilmsinIntegratedVertical-FlowConstructedWetlandWUZhen-bin1,LIJin1,2,CHENGShui-ping1,FUGui-ping1,HEFeng1(1.StateKeyLaboratoryofFreshwaterEcologyandBiotechnology,InstituteofHydrobiology,ChineseAcademyofSciences,Wuhan430072,Hubei,China;2.GraduateSchooloftheChineseAcademyofSciences,Beijing100039,China)　　Abstract:Spacialdistribution,dehydrogenaseactivities,respiration,TOCoftheinterstitialwater,developmenttimeandPCPdegradationinseparatelayerssamplesofthesubstrateofIntegratedvertical-flowconstructedwetlandweremeasured.Resultsindicatedthatthebiofilmsofthesubstratedevelopedwell,anditsbiomassreachedto0.0218.Indown-flowchamber,biomassofbiofilms,respiration,dehy-drogenaseactivitiesanddegradationcapabilityoforganiccontaminationwereallhigherthanthoseinup-flowchamber,anddown-flowchamberisthemainpurifyingspaceoforganicinwastewater.Thespacialdistributionofbiofilmsindifferentlayerswasdifferent,andbiofilmswereaccumulatedmainlyin0～10cmtoplayer,dehydrogenaseactivitiesandrespirationintoplayerwerestrongerthanthoseinotherlayers,thedistributionofalldatumshowedpositivecorrelation.D