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,＊,,,(, 430072):.,7.5×103～1.1×105MPN·g-1,7.5×106～1.1×107MPN·g-1.0.01～6.35μg·(g·d)-1,3.37～4.19μg·(g·d)-1.,(r=0.9661,p0.001).,,(r=0.7722,p0.025).,(r=-0.9776,p0.001),.:;;;;:X799.3 :A :0250-3301(2005)01-0047-04:2004-01-13;:2004-03-28:(863)(2002AA601021);(39925007);(KSCX2-SW-102):(1973～),,,.＊NitrificationandDenitrificationintheIntegratedVerticalFlowConstructedWet-landsHEFeng,WUZhen-bin,TAOJing,CHENGShui-pingFUGui-ping(StateKeyLabofFreshWaterEcologyandBiotechnology,InstituteofHydrobiology,ChineseAcademyofSciences,Wuhan430072,China)Abstract:Nitrificationanddenitrificationinthedifferentlayersoftheintegratedverticalflowconstructedwetlands(IVCW)arestud-ied.TheresultsshowtheconstantsrateofnitrificationanddenitrificationinthestrataofIVCWwere0.01～6.35μg·(g·d)-1and3.37～4.19μg·(g·d)-1respectively.Themostprobablenumber(MPN)methodareemployedtodeterminethenumberanddistri-butionofnitrifyinganddenitrifyingbacteriainthestrataofthesystem.Theresultsshowthatthenumbersofnitrifyinganddenitrify-ingbacteriawere7.5×103～1.1×105MPN·g-1and7.5×106～1.1×107MPN·g-1respectively.Thepositivecorrelationbe-tweenbacteriaandtheiractionwasobvious(r=0.9661,p0.001,r=0.7722,p0.025).ItisalsoobservedthatthenumberofnitrifyingbacteriaandnitrificationratewasdecreasedalongthedirectionofthewaterintheIVCW,whiledenitrifyingbacteriaanddenitrificationratewasincreased.Andtherewasthesignificantnegativecorrelationbetweennitrificationanddenitrificationrate(r=-0.9776,p0.001).Keywords:integratedverticalflowconstructedwetland;nitrification;denitrification;nitrifyingbacteria;denitrifyingbacteria 70,,,,,,,,、,[1～4].、、,[5].,,[5].,.(IntegratedVer-ticalFlowConstructedwetland,IVCW)“”、.,、,、[6].,.1 1.1 26120051 ENVIRONMENTALSCIENCEVol.26,No.1Jan.,2005DOI:10.13227/j.hjkx.2005.01.011,,(1).:,.:,.1 Fig.1 AschematicdiagramofIVCW1.2 1,PVC,.,1.(),1.,0.8m3/(m2·d),4,5h.1.3 ,4,1～10cm,10～20cm,20～30cm,35～45cm.,.1.4 [8]0.5mol/LK2SO4,.TN,NH+4,NO-3NO-2.[9].1 IVCW()[7]Table1 ParametersofinflowoftheMSP(Meanvalues)/℃pH/μs·cm-1/mV/mg·L-1CODcr/mg·L-1BOD5/mg·L-1TSS/mg·L-1KN/mg·L-1TP/mg·L-121.27.934718.23.422.34.41.94.60.368.00.75240.61.68.41.22.92.40.171.5 MPN,[10].1.6 10g,(1mg/N),,,,30℃2.,1∶50.5mol/LK2SO4,NO-3,.NO-3.1.7 10g,(1mg/N),,,,30℃40h.,1∶50.5mol/LK2SO4,NO-3,.NO-3.2 2.1 2,0～10cm,6.35μg·(g·d)-1,,,,,0.01μg·(g·d)-10.03μg·(g·d)-1.,,0～10cm0.33μg·(g·d)-1.2 Fig.2 Spatialvariationofthenumberofbacteria,nitrificationanddenitrificationrateinstrataofIVCW ,.0～10cm,1.1×105MPN·g-12.5×104MPN·g-1,35～45cm7.5×103MPN·g-1.3,48 26、,,,.3,368.8%～83.2%.,.3 Fig.3 SpatialvariationofextractableNinstrataofIVCW2.2 2,,0～10cm,3.37μg·(g·d)-1,,,4.11μg·(g·d)-14.19μg·(g·d)-1.,4.11～4.19μg·(g·d)-1.,,.,.,85%,,,[11,12].,.2.3 2,,,.,(r=-0.9776,p0.001),.ρ95%(-0.9660,-0.8772). ,,,,,,2,[3,4].:NH+4NO-2NO-3NO-2N2,.,,,[13].-,-,.,.,,,.,,.2.4 2,.,,(r=0.9661,p0.001;r=0.7722,p0.025),.0.9334,0.5962,,,.491 3 ,..,,,..,,.致谢:,,,,,,,,,,,,.:[1] ,.———[J].,1993,14(2):39～43.[2] ,,,.[J].,1991,10(9):41～44.[3] .[J].,1994,16(3):83～86.[4] InternationalWaterAssociation.ConstructedWetlandsforPol-lutionControl,Processes,Design,andOperation[M].Lon-don:IWAPublishing,2000.[5] ,,,.[J].,2003,24(1):80～83.[6] ,,,.[J].(),2003,36(1):12～16.[7] ,,,.[J].,2002,22(7):1012～1017.[8] .()[M].:,1996.[9] .[M].:,1985.[10] 《》.()[M].:,1989.[11] AlbertJO.Nitrogenremovalinconstructedwetlandstreatingnitrifiedmeatprocessingeffluent[J].WaterScienceandTech-nology,1995,32(3):137～147.[12] LinYF,JingSR,WangTW,etal.Effectsofmacrophytesandexternalcarbonsourcesonnitrateremovalfromgroundwaterinconstructedwetlands[J].EnvironmentalPollution,2002,119(3):413～420.[13] KozubDD,LiehrSK.Assessingdenitrificationratelimitingfactorsinaconstructedwetlandreceivinglandfillleachate[J].WaterScienceandTechnology,1999,40(3):75～82.50 26