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11*210009Pseudomonasstutzeri。SVI38~55mL/gVSS2.0g/LNO3--NNO2--N0.9kgNO3--N/m3·dNO3--NNO2--NNO3--NNO2--NNO2--NDO0.5mg/LpH9.3pH。PseudomonasstutzeriNO3--NNO2--NX703.1A1003-6504(2008)11-0005-04CultureofComplexDenitrificationSludgeandItsDenitrificationPropertiesCHENYing-wenCHENYangSHENShu-bao*NationalEngineeringResearchCenterforBiotechnologyNanjingUniversityofTechnologyNanjing210009ChinaAbstractWiththepurposetosolvetheproblemsinthebio-denitrificationprocessastrainwithdenitrificationabilitywasseparatedandidentifiedasPseudomonasstutzeri.Complexdenitrificationsludgewasculturedbythecouplinggrowthofthestrainandbio-activecarbonsludgeandthedenitrificationpropertieswerestudied.Resultsshowedthatthemicrophotoshapeofthecomplexdenitrificationsludgewasgranularirregularlywithnofilamentousbacteria.SVIwas38~55mL/gandVSSwas2.0g/L.AsforthedifferentdenitrificationsubstratessuchasNO3--NandNO2--Nthedenitrificationcurvesweresimilar.TherewassubstratescompetitiverelationshipbetweenNO3--NandNO2--NwhentheycoexistedinthesamesimulatedwastewaterbutnoinhibitiveeffectoccuredbyNO2--N.WhenDOwasbelow0.5mg/LthedenitrificationeffectwasgoodandinthehighpHof9.3denitrificationcouldtakeplacewell.Thevolumeloadingcouldgetto0.9kgNO3--N/m3·d.KeywordsdenitrificationPseudomonasstutzericomplexsludgenitratenitriteEnvironmentalScience&Technology3111200811Vol.31No.11Nov.20082008-01-192008－03－14“863”2006AA02Z2112006-1-W-19BK2006181BK200718820070201978-*025-83587349mrenglishchen@126.com。NO3--NNO2--NN2。NO3--N、NO2--NNO3--N、NO2--NN2[1]。DONO3--N、NO2--NCODNO3--N、NO2--N。、、[2-7]。NO3--N、NO2--NCOD。11.116SrDNAPseudomonasstutzeri99.88%Pseudomonasstutzeri。NO3--N、NO2--N。DOI:10.19672/j.cnki.1003-6504.2008.11.002310.5%。15d。[8]、。、。CH3COONa2.55g/LNaNO34.55g/LKH2PO40.135g/LMgSO4·7H2O1.5g/LCaCl2·2H2O0.90g/L。CH3COONa1.02g/LNaNO30.91g/LKH2PO40.027g/LMgSO4·7H2O0.3g/LCaCl2·2H2O0.18g/L。CH3COONa1.02g/LNaNO20.74g/LKH2PO40.027g/LMgSO4·7H2O0.3g/LCaCl2·2H2O0.18g/L。。1.2[9]NH4+-NNO3--NNO2--NN-1--CODpHpHLeicaDM1000DOJENCO9250MVSS、SVIPEλ25。pH、DO、。22.11.5L0.25LMLSS=3g/L15g0.5%15d12h10min5min3min1210min345min563minSVI120~150mL/g38~55mL/gVSS2.0g/L。[10]。1。2.2NO3--N、NO2--NNO3--N、NO2--N100mg/L2.0g/L25~30℃DO0.5mg/L。2,3。。23NO3--N50%NO3--N、NO2--N3.5hNO3--N、NO2--N100%△COD/△N3/1NH4+-NNH4+-N。2.3DO2.2HRT=3.5h1125~30℃DODODO。4DO0.5mg/LCOD20%DO1.0mg/LCOD10mg/L96%DO1.0mg/LO2DO0.5mg/LNO3--NO2NO3--N、NO2--N、NH4+-NCOD。△COD/△N3/12.2。DO0.75mg/LNO3--N、NO2--NCODO2NO3--N。NO3--N、NO2--NNO3--NDODO0.75mg/LNO3--N87%。DO0.75~2.0mg/LNO2--NNO3--NNO2--NNO2--NO2DONO[1]。DO4.0mg/LCOD。DO0.5mg/L0.75mg/L。2.4NO3--N、NO2--N2.3DO0.5mg/LNO3--N、NO2--N。5。5NO3--N、NO2--N。0~0.5hNO2--NNO3--N。NO2--N0.5~3hNO3--N、NO2--NNO2--NNO3--NNO3--N10mg/LNO3--N、NO2--NNO2--N。NO3--N2NO2--N[11]。NO2--NNH4+-NNO3--NNO2--N4DO0.5mg/LNO3--NNO2--N。2.5pHNO3--NDO＜0.5mg/LpH。6,7。6pH。0.5hpH＞8.53pH10.1。7pHpH9.35。67pH31pHpH＜8.5。2.6NO3--N、DO0.5mg/LNO3--N、。8。50d0.9kgNO3--N/m3·d、4.14kgCOD/m3·dNO3--N＜5mg/LCOD＜50mg/L。NO3--N。31NO3--N、NO2--N16SrDNAPseudomonasstutzeri。2PseudomonasstutzeriSVI38~55mL/g。3NO3--NNO2--NNO3--N50%NO3--N100%。4DO0.5mg/L。DO0.75mg/LNO3--N87%。DOO2。5NO3--N、NO2--NNO3--NNO2--N。6pHpH＜8.5。7N、C0.9kgNO3--N/m3·d、4.14kgCOD/m3·dNO3--N＜5mg/LCOD＜50mg/L。、。[][1].[M].2004:5670.ZhengPingXuXiang-yangHuBao-lan.NovelBio-technologyandPrincipleforNitrogenRemoval[M].BeijingSciencePress2004:5670.inChinese[2]SongSeungHoonChoiSukSoonParkKyungmoon,etal.Novelhybridimmobilizationofmicroorganismsanditsapplicationstobiologicaldenitrification[J].EnzymeandMicrobialTechnology,200537567-573.[3]KesserPKissIBihariZetal.Biologicaldenitrificationinacontinuous-flowpilotbioreactorcontainingimmobilizedPseudomonasbutanovoracells[J].BioresourceTechnology,20038775-80.[4]KesserPKissIBihariZetal.Investigationofthedenitri-ficationactivityofimmobilizedPseudomonasbutanovoracellsinthepresenceofdifferentorganicsubstrates[J].WaterResearch,2002361565-1571.[5]CastillaCMToledoIBFerro-GarcíaMA,etal.Infl-uenceofsupportsurfacepropertiesonactivityofbacteriaimmobilizedonactivatedcarbonsforwaterdenitrification[J].Carbon,2003(41)1743-1749.[6]LiuDe-liZhangShanZhengYong-liang,etal.Denitri-ficationbythemix-culturingoffungiandbacteriawithshell[J].MicrobiologicalResearch,2006,(161)132-137.[7].[J].,200426(3)318-321.TianJin-hongXiongPing.Denitrificationcharacteristicsofimmobilizeddenitrifyingbacteria[J].JournalofSouthwestAgriculturalUniversity,2004263318-321.inChinese[8]BeunJJVanLoosdrechtMCMHeijnenJJ.Aerobicgranulationinasequencingbatchairliftreactor[J].WaterResearch,2002,(36)702-712.[9].[M].1989:252-274354.StateEnvironmentalProtectionAdministration.AnalyticMethodofWaterandWastewater[M].BeijingChinaEnviromentalSciencePress1989:252-274354.inChinese[10].[J].,200636(5)817-820.LiuXiao-yunWangLinLinYue-mei.Applicationoftransmissionelectronmicroscopetechniquestostudyingtheaerobicsludgegranularultrastructure[J].PeriodicalofOceanUniversityofChina2006365817-820.inChinese[11].[M].2003:362.ZhaoYou-cai.EnviromentalEngineeringChemistry[M].BeijingChemicalIndustryPress2003:362.inChinese