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[1-2]。2[3-4]2212。2。N2。22。。Thiosphaerapanatotropha、Microvirgulaaerodenitrificans[5-6]Rhodococcus[7]Delftia[8]。、、。2。BalaKiran[9]。3[10]--。DosSan-tos[11]、[12]。、。11.1SHA-BYXQG02LD4-2A722SL-CJ-1N。1.2150mg·L-11。122111.3001302.300130、。2.5%1%32℃pH8.088.35%Monodvmax=2.47×10-3mg·L-1·s-1Km=481.38mg·L-1。X703.1A1000-3770(2011)08-0048-0052010-10-2108220502ZZ2009411983－E-maillifang6168@163.comE-mailqishuting@yahoo.com.cn37820118TECHNOLOGYOFWATERTREATMENTVol.37No.8Aug.,201148DOI:10.16796/j.cnki.1000-3770.2011.08.0121.3、、。5100mL30℃3d3000r·min-110min310mL1.5×108～1.6×108mL-1。1.4、、。31100mL150mg·L-130℃、120r·min-148h。1.58g、2g90mL。30℃2%CaCl24mm24h。22.11。3131。13282.32%0.032mg·L-10.053mg·L-1。32。2.2A、B、CpHD4323。3MMA3B1C3D22.5%1%32℃pH1、Fig.1Nitrite,nitrateaccumulationandremovalrateofammonianitrogenofcombinationbacteria0.000.020.040.060.080.100.120.140.16mg L mL mL % 1:11:21:32:1 2:3 3:1 3:201020304050607080901Tab.1ThemediumofexpermentsNaNO2NaH2PO4MgSO4K2HPO4NaCO3C4H4O6KNaKNO3K2HPO4MgSO4·7H2OpH20g2g0.5g0.2g1000mL7.2NaClKClCaSO4MgCl2MgSO4·7H2ONH42SO4NaClK2HPO4MgSO4·7H2ONaHCO32g0.3g1g0.03g1.6g1000mL1g0.25g0.03g0.65g1g1000mL27.403g0.7627g1.3995g6.7816g4.4243g1000mL1352830327.58.08.51231.52.02.5C/℃DA/%B/%2Tab.2Factorsandlevelsoftheorthogonaltest498.088.35%R＞pH＞＞。2.32.3.1rdrFick4π(r+dr)2De(dρSdr)r+dr-4πr2De(dρSdr)r=4πr2drvmaxρSKm+ρS1vmaxKmρSDe。drdr→0d2ρSdr2+2·dρSr·dr=vmaxρSDe(Km+ρS)2r=RρS=ρS0r=0dρSdr=0。rRρS0。r軃=rRρ軃S=ρSρS0β=ρS0Km。r軃ρ軃Sβ。2d2ρ軃Sdr軃2+2·dρ軃Sr·dr=9Φmρ軃S1+βρ軃S3Φm=R3vmaxKmDe姨Thiele。r軃=1ρ軃S=1r軃=0dρ軃Sdr軃=0。3。。2.3.2η=vsv04ηvsv0。4η=vsv0=R0乙4r2vmaxρSKm+ρSdr43πR3vmaxρS0Km+ρS055η=3RDedρSdr乙乙r=0vmax·ρS0Km+ρS067[13-14]η=η0+a(KmρS0)bη11+a(KmρS0)bη17a=2.6b=0.8。η0η1。0Φ00.577η0=0Φ0=R32姨vmaxDe·ρS0姨8Φ00.577η0=1-(12+cos(φ+4π3))3912312312373.80067.71772.0906.08312323131268.48370.36374.7606.277123456789M1jM1jM1jRj11122233357.40777.57378.62721.220A/%B/%C/℃12331223167.25774.77071.5807.51353.3456.6562.2379.7173.7079.3188.3572.8074.73D/%3Tab.3Analysisoftheorthogonaltestdata378503Fig.3Fittingcurveofmodel0123456789100500100015002000250030003500 v /L s mg S /10 L mg φ=cos-1(23Φ02-1)Φ0=R32姨vmaxDeρS姨姨姨η1=Φ0=1Φ1(1tan(3Φ1)-13Φ1)10Φ1=R3vmaxDeKm姨11η。Km/CS08[15]10。2.3.34mm42。120r·min-1A3B1C3D2。2v=ρi-ρb3600t2.155×10-3mg·L-1·s-1ρs051.33mg·L-1De[16]0.216×10-2m·s-1。De、vs、ρS0R11Φ=0.2930.3[17]。4mm。2.3.4KmVmax。Monod1v=Kmvmax1S+1vmax43Monod。Y1/vmaxKm/vmax。vmax=2.47×10-3mg·L-1·s-1Km=481.38mg·L-1。。31/S1/v0.99734mm、。origin753212.7%2Fig.2Experimentofammoniumnitrogendegradation048121620242832020406080100120140160mg L min-421.79506.57596.52655.94746.63956.181455.531963.642500.003312.87v-1/L·s·mg-1(v-v)/v×100/%--7.25-10.71-11.40-1.109.0512.700.14-6.121.15-0.95/hρ()/mg·L-1S-1/10-2L·mg-1036912151821242730150.24122.8399.0380.1962.8247.2633.8924.6719.0513.6710.41150.24122.8399.0380.1962.8247.2633.8924.6719.0513.6710.41v/10-3mg·L-1·s-1-2.371.971.681.521.341.050.690.510.400.304Tab.4Effectofdenitrificationbyimmobilizedbacteria51STUDYONKINETICSMODELOFREMOVALAMMONIANITROGENWITHCOMPLEIMMOBILIZEDBACTERIALiFangfang1,QiShuting2,ShiYuxin2,LiJunchong1,LvYushan1（1.DepartmentofBioengineering,HebeiUniversityofTechnology,Tianjin300130,China;2.EngineeringResearchCenterofSeawaterUtilizationTechnology,MinistryofEducation,HebeiUniversityofTechnology,Tianjin300130,China）Abstract:Thenitrosobacteriaanddenitrobacteriawereimmobilizedinthecarriermadeupofpolyvinylalcohol,sodiumalginateandactivatedcharcoaltoconductthenitrificationanddenitrificationexperiment.Conditionsoftheexperimentwereoptimizedbyusingorthogonaldesign.Fordeterminationofdiffusivepropertiesofthecarrier,animageanalysiswasapplied,thuskineticsmodelwasfounded.ItwasrevealedthattheremovalefficiencyofNH4+-Nwasthehighestunderthecarrierconditionsof2.5%bacterialsuspensionconcentration,1%ofactivatedcharcoal,32℃ambienttemperature,pH=8.0.Undersuchconditions,theremovalefficiencycouldreach88.35%.Theinternaldiffusionintheexperimentcouldbeneglected.ThereactionkineticsofimmobilizedbacteriacouldbeexpressedasMonodequation,kineticparametervmax=2.47×10-3mg·L-1·s-1,Km=481.38mg·L-1.Keywords：compleximmobilizedbacteria;removalofammonianitrogen;orthogonalexperiment;kineticsmodel6.06%。33230℃、120r·min-148h82.32%0.032mg·L-10.053mg·L-1。。。432.5%1%32℃pH8.088.35%。4mm、Monodvmax=2.47×10-3mg·L-1·s-1Km=481.38mg·L-1。[1]PotterTG,TsengCC,KoopmanB.Nitrogenremovalinpartialnitrification/completedeniyrificationprocess[J].WaterEnvironmentResearch,1998,70(3):334-342.[2]LadanH,GeorgeN,AmarjeetSB,etal.LongtermperformanceofMBRforbiologicalnitrogenremovalfromsyntheticmunicipalwastewater[J].Chemosphere,2007,66(5):849-857.[3].[D].:,1993(7):192-197.[4]HoltJG,KriegNR,SneathpHA,etal.Bergey'smanualofdeterminativebacteriology[J].Baltimore:TheWilliamsandWikinsCompany,1994:447-450.[5]RobertsonLA,KuenenJG,KleijintjensR.AerobicdenitrificationandheterophicnitrificationbyThiosphaerapantotropha[J].AntounievanLeeuwenhoek,1985,51(4):445-448.[6]PatureauD,BernetN,DelgenesJP,etal.Effectofdissolvedoxygenandcarbon-nit