复合式厌氧氨氧化反应器脱氮性能研究高彦宁

3111201111ActaScientiaeCircumstantiaeVol．31No．11Nov．2011No．10972049SupportedbytheNationalNaturalScienceFoundationofChinaNo．109720491981—[e-mail]*[e-mail]1981—[e-mail]*[e-mail]hehybridanammoxreactorforbiologicalnitrogenremovalJ．ActaScientiaeCircumstantiae31112361-2367121*21．1160242．860-85552011-02-222011-05-272011-06-08．．38d1．9kg·m－3·d－1．17d3．8kg·m－3·d－1．180d6．6kg·m－3·d－10．32kg·kg－1·d－1．SEM．FISHAnammox70%．FISH0253-2468201111-2361-07X703．1AStudyontheperformanceofthehybridanammoxreactorforbiologicalnitrogenremovalGAOYanning12LIUZhijun1*FURUKAWAKenji21．FacultyofChemicalEnvironmentalandBiologicalEngineeringDalianUniversityofTechnologyDalian1160242．GraduateSchoolofScienceandTechnologyKumamotoUniversity2-39-1KurokamiKumamoto860-8555JapanReceived22February2011receivedinrevisedform27May2011accepted8June2011AbstractAhybridreactorforanaerobicammoniaoxidationanammoxprocesswasdevelopedwhichcombinedafixed－bedusingnon－wovenfabricsasbiomasscarrierwithaslowspeedagitatedbed．Theperformanceofhigherbiomassretentionwasobtainedinthereactorandtheanammoxmicroorganisminalooseflocculatingstructurebeforeinoculationformedrapidlyintogranules．Thenitrogenremovalabilityofthereactorincreasedobviouslyintheshorttermandthestart－uptimeofthereactorshortenedgreatly．Onlyin38daysthetotalnitrogenremovalrateTNRRreached1.9kg·m－3·d－1andthendoubledto3．8kg·m－3·d－1within17days．During180operatingdaysamaximumTNRRof6．6kg·m－3·d－1wasobtainedandthespecificanammoxactivitySAAmaintainedbasicallyin0．32kg·kg－1·d－1．ThetestingresultsofsizedistributionandSEMobservationconfirmedthatanammoxsludgegranulationprocesscanbegreatlyenhancedinthemechanicalstirredreactoratalowerrotatingspeed．Theanammoxbacteriacontentinthemicroorganismsludgewasabout70%determinedbysituhybridizationFISHanalysismethod．KeywordsAnammoxreactorslowspeedagitatedbedfixedbedFISH1IntroductionAnaerobicammoniaoxidationAnammoxMulderetal．1995VandeGraafetal．1995．Jettenetal．2005．Anammox11dStrousetal．1999、DOI:10.13671/j.hjkxxb.2011.11.01231Furukawaetal．2003．．Strousetal．1997、Fujietal．2002PVATsungetal．2008．．Strousetal．1997VandeGraafetal．1996．Sliekersetal．2003Imajoetal．2004Strousetal．19981999DapenaMoraetal．2004．．Strousetal．1998．Strousetal．1997．．Tsushimaetal．2007Isakaetal．200726kg·m－3·d－111kg·m－3·d－1．50%．．AnammoxAnammox．、、．2Materialsandmethods2．16L1．．、．．1Fig．1StructureofthehybridAnammoxbio-reactor2．2AnammoxMLSS4．2g·L－1．KHCO3HCO－320minAnammox．．．2632112．3．NH42SO450～300mg·L－1、NaNO250～300mg·L－1、KH2PO4189mg·L－1、KHCO3187．5mg·L－1、FeSO4·EDTA1．0～1．5mL·L－1FeSO418g·L－1EDTA10g·L－1、2mL·L－1NaCl0．5g·L－1CaCl2·2H2O0．7g·L－1KCl0．7g·L－1MgSO4·7H2O0．5g·L－1．15min1mg·L－1．2．435℃180d．421d30min5min2230r·min－13980r·min－13h9680r·min－1．8h1．5h30～80r·min－10．07～0．35m·h－10．2～0．5m·s－1．2．5Kanda1995．APHA1995．SSMLSSMLVSS．DOpHD-55pHB-211．AnammoxSAASpecificAnammoxActivityDapena-Moraetal．2004．6g60mL100mL70mg·L－1．35°C100·min－1．1h、．．ECLIPSEE600Anammox．LA-920、、3．SEMJSM-6390LV．FISHGlockneretal．1996Schmidetal．2005．AnammoxAm8205'-CAAAACCCCTCTACTTAGTGCCC-3'．DAPI．3Resultsanddiscussion3．1180d2．AnammoxHRT8h．21d1．2kg·m－3·d－10．9kg·m－3·d－1．216030r·min－1HRT3h．2．3kg·m－3·d－11．9kg·m－3·d－1．60dHRT3h30r·min－180r·min－1．964．6kg·m－3·d－13．5kg·m－3·d－1．Anammox．Trigoetal．2006．34．3632312Fig．2Nitrogenremovalperformancesduring182operatingdaysofhybridreactor3Fig．3Trendforgranularsludgesizedistributioninhybridreactor．．．Strous1997SBR15%．70%130d6．1kg·m－3·d－119．6%．HRT3h1.5h．1688．9kg·m－3·d－16．6kg·m－3·d－1．SAA0．32kg·kg－1·d－1．4Fig．4Dominantgranulesizeandpercentageineveryphase3．234Anammox4632110．26mm0．78mm．HRTHRT．HRT．5SEM．5a5b5cAnammoxStrousetal．1997Arrojietal．2006Gongetal．2007Tangetal．2009FISHGongetal．2007Anammox．．5b．5dAnammoxAnammox．5SEMa．b．2000c．5000d．Fig．5TheSEMgraphofsludgesamplestakenfromhybridreactora．surfaceofgranuletakenfromfluidizedbedb．innerofgranuletakenfromfluidizedbed2000c．innerofgranuletakenfromfluidizedbed5000d．sludgeattachedonnonwovenfabrics3．3FISHFISHFISH6．AnammoxAnammox70%．AnammoxGongetal．20075a5b．3．4．Dapena-Mora2004SBR70r·min－10．82mm0．44kg·kg－1·d－1Arroji200660～90r·min－10．6～0．8mm0．4kg·kg－1·d－1．56323130～80r·min－10．6～0.78mm0．32kg·kg－1·d－1．6．6kg·m－3·d－1Dapena-Mora20040．75kg·m－3·d－10．79kg·kg－1·d－1Arroji20060．2kg·kg－1·d－1、．6FISHac．bd．Fig．6FISHimageoftworandomlyselectedsectionsofgranularsludgetakenfromhybridreactorwithDAPIstainfortotalmicrobesandprobeAm820labeledwithfluoresceinisothiocyanateFITC-12-dUTPgreenforanammoxbacteriaa．totalmicrobesinsectionAb．anammoxbacteriainsectionAc．totalmicrobesinmiddlesectionBd．anammoxbacteriainsectionB．．．6a6bSEM．．SSSS30mg·L－1．8%．8%．SBR、、Anammox．Strousetal．1997．Dapena2004SBRSVI90mL·g－1110mL·g－1．SVI30mL·g－118mL·g－1．．FISHSBRSBR663211Dapena-Moraetal．2004Arrojoetal．2006Jinetal．2007Arrojoetal．2008．4Conclusions1．38d1．9kg·m－3·d－1．180dHRT1．5h80r·min－16．6kg·m－3·d－10．32kg·kg－1·d－1．2SEM．38%．4．5、Anammox．1969—、、．ReferencesAPHA．1995．Standardmethodfortheexaminationofwaterandwastewa