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1,2,1,1,1,1,1(1.,150090;2.,075024):,,4,F2F6,,:;;:X172:A:1000-4602(2003)04-0001-04DevelopmentofComplexMicrobialFlocculantMAFang1,LIUJun2liang2,LIShu2geng1,YANGJi2xian1,ZHANGLi2qiu1,WUBo1,ZHUYan2binl(1.SchoolofMunicipalandEnvironmentalEngineering,HarbinInstituteofTechnology,Harbin150090,China;2.DeptofUrbanConstruction,HebeiInstituteofArchitecturalEngineering,Zhangjiakou075024,China)Abstract:Thecomplexmicrobialflocculantwasdevelopedbyusinginexpensivesubstratethroughfermentation.Thebacteriausedinfermentationwerescreenedfromactivatedsludgeandsoil,ofwhichfourstrainswereidentifiedasBacillus.Aftertwoarbitrarystrainsweremixedandcultivat2ed,itwasfoundthatthemixedfermentationliquidofF2andF6hasmoreefficiencythanthesinglestrain.Therefore,thetwostrainswereusedinthefollowingexperiment.Fermentationconditionfortheflocculantweredeterminedbymeansofoscillatortestanditsflocculationefficiencyforrawwaterwasalsomeasured.Keywords:complexmicrobialflocculant;fermentationcondition;watertreatment:(GB02C202-02),DNA,,[1][2](),,,12003Vol.19CHINAWATER&WASTEWATERNo.41111:;:1000mL,10g,K2HPO45g,MgSO47H2O0.2g,0.5g,KH2PO42g,NaCl0.1g,0.5g,pH7.5112,,,3024h:50mL0.2g1mLCaCl2(1%)1mL,50mL,15min,,:1000mL,10mL,10mL1.5mLCaCl2(10%)20min,,[3]:(%)=(A-B)100/A(1)AB113,114,115CaCl2,CaCl2,CaCl2,,CaCl2(10%)116,221125,,4(1)1(%)F284F381F583F683,4,(11),,22%F2+F3F2+F5F2+F6F3+F5F3+F6F5+F673.274.993.185.670.481.2480%,2,,,[4],,,,,,,F2F6,,2F2F6,212F2F6,(Bacillus),33G+++MR+VP---+22003Vol.19No.421321311:,,;;,,,(),[5],,21312;,,,,,,,111,5d,5d2,,,,5d,5d,,22,15(80%),,,1d213131520253035,,,333,,3085.2%,1542.1%,35,,,3021314[67],(,),,,,,,,44,90105r/min60%,120r/min32003Vol.19No.485.2%,135r/min87.5%,,,120r/min4214CaCl2CaCl255CaCl25,CaCl2,CaCl20.5mL/L1.5mL/L,CaCl2,3mL/L,,,CaCl21.5mL/L215UV254,44,,,,,4(%)(NTU)263.487()401270(/mL)185056070UV254(cm-1)0.2060.140323,,,,:[1].[J].,2000,22(2):18-21.[2],,,.[J].,2002,33(1):7-9.[3],,.[J].,2002,35(3):5-7.[4],.[M].:,2002.[5],.[M].:,1996.[6],.[J].,2001,23(2):61-62.[7],,,.[J].,2001,95(3):28-31.:(1963-),,,,,:13904810011:2003-01-27::42003Vol.19No.4