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24220076JournalofGuangdongUniversityofTechnologyVol.24No.2June2007:2006209218:(19792),,,.1,2,3,4(1.,510090;2.,510090;3.,710072;4.,200090):,,,,.,1.5%1.7%,3%,4.6%5%,1.1%1.3%,55.3mL,94%.:;;;;;:S949:A:100727162(2007)0220015205,,,.,,.,[1].,,,,,,,,,,.,[2],.,,[325]..,,.,,,,,.11.1:,,90%;:25%,,;:;:;:,;:(9109/mL):;HJ-4:;:;721:.1.2,.1.2.1(Ca2+),,.GCa2+,G,[6],,.,,..,shiff,,.1.2.2119.1,50mL,50mL10mL,,,,,412h,19,.1x1()/%x2(CaCl2)%x3()/%x4()/%x5/mL10.51.523420.53.031430.53.013341.05.021351.07.033261.07.040.5271.01.512681.51.520.5491.53.0324101.53.040.53111.55.0123122.05.020.52132.07.0312142.07.0436150.51.5314160.51.510.52171.03.0213181.03.0314191.55.03241.3K2HPO452mgMgSO47H2O25mgNaCl125mgMnSO42.5mgFeSO47H2O2.5mgNH4Cl50mg500mg1L,NaHCO3pH7.5.1.4,.1501L,.,,3,.1.5,.,72h,.[7].21,,2.2(NH+4)/(mgL-1)24h48h72h/%114.2112.066.351.8187.3214.2110.686.111.6288.6314.2111.236.252.4982.5414.217.533.141.4789.7514.2110.56.950.9793.2614.21138.162.8080.3714.219.717.632.6581.4814.2111.777.982.0185.9914.216.924.011.8287.21015.247.714.540.9793.61115.249.653.480.8194.71215.241.160.970.7094.71315.247.472.640.8994.21415.249.894.961.0293.31515.243.472.371.1492.51615.246.463.691.1792.31715.2411.486.911.0593.11815.2412.757.131.2491.91915.243.112.260.8194.72.1,Vis2ualBasic6.0,Windows,,.195(1)(2)(196),y=4391177-3531823x1+3611757x21-1041646x31-311935x2+101417x22-11001x32+431501x3-241839x23+41069x33+3151081x4-205149x24+381793x34-3021708x5+741355x25-51712x35,=4145,9815%,..2.2,xi(i=12345,xi123),yxi(15).5xy,y46124,.2.2.11,0.7%,,,1.6%,,,.,,,;,,,.1.5%1.7%.()/%1Y2.2.22,,,.,,.,,,,,.4.6%5%,.(CaCl2)/%2Y2.2.33,,,,,.,.,.,,.,3%.()/%3Y2.2.44,,,.,,,,,,1.1%,,,,,,,,.,1.1%1.3%.()/%4Y2.2.55,3mL,,,..55.3mL.712,:5Y2.3,,,,,.,,,.,(Oxic),,,(Anoxic),()(N2).1L,,3.3mgL-124h48h72h24h48h72h10.050.560.060.650.030.5620.160.580.150.620.430.5930.080.690.140.660.350.3440.150.620.240.350.360.5350.250.540.290.540.260.2960.230.460.270.460.240.3570.380.410.320.730.350.3980.270.290.160.680.310.3390.160.370.130.610.260.57100.360.530.430.510.220.45110.310.160.280.560.300.44120.400.280.160.340.330.36130.290.190.170.420.160.39140.180.340.260.460.190.43150.090.550.230.590.270.40160.280.610.240.540.120.36170.420.360.270.550.220.33180.580.240.130.640.350.42190.490.510.240.350.230.443,,,,.,,,.,,.2.4,,,45,94%.4(,)/(mgL-1)(,)/(mgL-1)/%114.920.79194.7214.920.67395.5314.920.73195.1414.920.76994.8514.920.71695.2812431),,94%.2):1.5%1.7%,3%,4.6%5%,1.1%1.3%,55.3mL.3),,,..:[1]ANTONIOT,CARLOSM,MANUELPM,etal.Environ2mentalimpactsofintensiveaquacultureinmarinewaters[J].WatRes,2000,34(1):3342342.[2]KIMSung2koo,KONGInsoo.Removalofammonium2Nfromarecirculationaquaculturalsystemusinganimmobi2lizednitrifer[J].AquaculturalEngineering,2000,21(3):1392150.[3],,,.[J].,2004,19(4):2812286.[4]SEOJae2koan,JUNG2hyong,KIMMi2ryung,etal.Ni2trificationperformanceofnitrifiersimmobilizedinPVAforamarinerecirculatingaquariumsystem[J].AquaculturalEngineering,2001,24(3):1812194.[5]BOLEYA,MULLERWR,HAIDERG.Biodegradablepolymersassolidsubstrateandbiofilmcarrierfordenitrifi2cationinrecirculatedaquaculturesystems[J].Aquacul2turalEngineering,2000,(1)22:75285.[6].[M].:,2002.86287.[7]().[M].:,2002.2792281.RemovingAmmoniaNitrogenfromWastewaterbyImmobilizationNitrobacteriawithChitosanandAlginateQISu2fang1,YUYu2mian2,LAIZi2ni3,GAOPeng4(1.FacultyofEnvironmentalScienceandEngineering,GuangdongUniversityofTechnology,Guangzhou510090,China;2.FacultyofChemicalEngineeringandLightIndustry,GuangdongUniversityofTechnology,Guangzhou510090,China;3.FacultyofMaterial,NorthwesternPolytechnicalUniversity,Xian710072,China;4.FacultyofLifeScience,ShanghaiFisheriesUniversity,Shanghai200090,China)Abstract:ThispaperistofindoutthebestconditionoftheimmobilizedmicrobialbeadstotreatNH+42Ninfisherywastewater,whichismadefromentrappingnitrobacteriawithchitosanandalginateasentrapmentcarriers.Theex2perimentresultsshowthatwhenthechitosanmasspercentis1.5%1.7%,alginatemasspercent3%,CaCl2masspercent4.6%5%,glutaradehydemasspercent1.1%1.3%,nitrobacteria55.3mL,theNH+42Nremovalefficiencyoffisherywastewaterisabove94%.Keywords:chitosan;alginate;nitrobacteria;immobilizationtechnology;fisherywastewater;removalofammonianitrogen912,: