高温ASBR处理热水解污泥的梯度升温法启动试验研究欧阳二明

ASBR1,2,1＊,3,4(1.,100084;2.,330031;3.,100083;4.,710054):、ASBR.,131dASBR.3(35℃※40℃※47℃※53℃),,※(35℃※40℃)、※(47℃※53℃),※(40℃※47℃)、、COD,,40℃47℃.,2.038Ld,72.0%,COD(CH4COD)188.8mLg,TCODSCOD63.8%83.3%.DGGE,,(35℃)(53℃).:;;;;PCR;DGGE:X705　:A　:0250-3301(2009)04-1105-05:2008-05-27;:2008-06-24:(863)(2006AA06Z384):(1976～),,,,.＊,E-mail:[e-mail]peratureStartupExperimentofThermophilicASBRTreatingThermal-HydrolyzedSewageSludgeOUYANGEr-ming1,2,WANGWei1,LONGNeng3,LIHuai4(1.DepartmentofEnvironmentalScienceandEngineering,TsinghuaUniversity,Beijing100084,China;2.SchoolofCivilEngineeringandArchitecture,NanchangUniversity,Nanchang330031,China;3.SchoolofCivilandEnvironmentalEngineering,UniversityofScienceandTechnologyBeijing,Beijing100083,China;4.DepartmentofEnvironmentalScienceandEngineering,ChanganUniversity,Xian710054,China)Abstract:Startupexperimentwasconductedforthermophilicanaerobicsequencingbatchreactor(ASBR)treatingthermal-hydrolyzedsewagesludgeusingthestrategyofthestep-wisetemperatureincrement:35℃※40℃※47℃※53℃.Theresultsshowedthatthefirststep-increase(from35℃to40℃)andfinalstep-increase(from47℃to53℃)hadonlyaslighteffectonthedigestionprocess.Thesecondstep-increase(from40℃to47℃)resultedinaseveredisturbance:thebiogasproduction,methanecontent,CODeffluentandmicroorganismallhavestrongdisturbance.AtthesteadystageofthermophilicASBRtreatingthermal-hydrolyzedsewagesludge,theaveragedailygasproduction,methanecontent,specificmethaneproduction(CH4CODinfluent),TCODremovalrateandSCODremovalratewere2.038Ld,72.0%,188.8mLg,63.8%,83.3%respectively.TheresultsofSEMandDGGEindicatedthatthedominantspeciesareobviouslydifferentatearlystageandsteadystage.Keywords:thermal-hydrolyzedsewagesludge;anaerobicsequencingbatchreactor(ASBR);gradientelevationoftemperature;startup;PCR;DGGE　　(ASBR)HRT()SRT()、MCRT()[1～3],[4～6].(thermalhydrolysis)[7～9],.“-”[10～12].ASBR,ASBR,PCR-DGGE.1　1.1　ASBR15cm、32.5cm.ASBR5L,4L,1L.30420094　　　　　　ENVIRONMENTALSCIENCEVol.30,No.4Apr.,2009DOI:10.13227/j.hjkx.2009.04.024,.,..1.2　.6(:3.2mm×3.2mm),170℃、30min.(3℃).ASBRASBR.1.,SCOD601mgL12434mgL,.1　Table1　Characteristicsofrawandthermal-hydrolyzedsewagesludgepH6.806.66(CaCO3)mg·L-18001021TSg·L-136.28033.056VSg·L-123.50620.646SSg·L-133.56123.033VSSg·L-118.66015.615TCODg·L-140.31738.857SCODg·L-10.60112.434VFAg·L-145423441.3　ASBR,TS24.748gL、VS12.272gL、SS23.668gL、VSS11.220gL,VSSSS0.474.3L,1LHRT20d、(TCOD)1.943kg(m3·d).ASBR24h,30min,20h,3h,30min.ASBR10minh.ASBR(35℃)(53℃):35℃※40℃※47℃※53℃.1～15d,ASBR35℃;16～29d40℃;30～91d47℃;92～131d53℃.1.4　pHOrion868pH;ZDJ-4A:(TS)、(VS)、(SS)、(VSS)[13];COD[13].SQ-206(FID);SQ-206(TCD);.FEIQUANTA200.1.5　DNA、PCRDGGEMPBiomedicals(FastDNA★RSPINforSoilKit)DNA,PCR.16SrDNAPCRPRBA8FPRUN518R,(PRUN518R)GC.PCRMJ-RESEARCHPTC-200PCR.:94℃7min;94℃1min;55℃30s,72℃1min,24;72℃10min.PCR1%,.510bpDGGE.PCR35%～60%(8%),D-Code(Bio-RAD).100V、60℃12h.,SYBRGold,(UVP)[14].2　2.1　(ASBR),,ASBR.1131d.,(),3L1L,,,,1d3.337L,2d2.680L,200mL(HRT=20d),,.35℃15d,40℃,(1.682Ld2.67Ld),1.600Ld,14d(40℃),,1.621Ld(40℃).29d,40℃53℃,,29d1.537Ld30d0.740Ld,,1106　　　　　　3036d(0.030Ld),,,,.,37～47d,(0.12Ld1.92Ld),.,,,,48～51d2.5Ld.52～91d,47℃,1.380～1.960Ld.92d,47℃53℃,.111～131d,,1.890～2.24Ld,2.038Ld,COD(CH4COD)188.8mLg,.,1～15d(35℃)、16～29d(40℃)、30～91d(47℃)、92～131d(53℃)72.3%、72.6%、60.2%、72.0%,40～47℃,.1　ASBRFig.1　DailygasproductionoftheASBR2.2　pHpH,pH.,pH..2ASBRpH.131d,pH6.97～7.94,40℃47℃,pH,,,,,pH.(111～131d),pH7.58～7.88,(CaCO3)5685～5795mgL(5739mgL),.2　ASBRpHFig.2　pHprofilesinoutflow2.3　COD3ASBRTCODSCOD.,TCOD5.609gL14.282gL,SCOD0.607gL2.146gL.47℃33d,TCODSCOD9.211gL1.403gL,,(40℃)(47℃),,COD.(111～131d),TCODSCOD13.862～14.282gL9.362～9.782gL,63.8%83.3%.3　ASBRSCODTCODFig.3　SCODandTCODprofilesinoutflow2.4　.ASBR,.11074:ASBR,VSS,,[15].4,,48.9nmolmL,35℃40℃,32.1nmolmL(18d),5d51.6nmolmL.35～40℃,,.2(40～47℃),:33d(23.3nmolmL),,48d31.7nmolmL;(48～89d),89d116.6nmolmL.3(47～53℃),,,125d124.3nmolmL.4　ASBRFig.4　MicroorganisminASBR2.5　(SEM)ASBR(35℃)(53℃),5.,ASBRSEM,.,,、,;,,、,,,,、.,DNA,PCRDGGE,6.1ASBR,2.2,26,5　ASBRFig.5　SEMphotoofthemicroorganismsintheASBR6　Fig.6　EubacteriacommunitystructuresintheASBRatearlystageandsteadystageofstartup1a、1b、1c、1e2a、2b、2c、2f,4;,,,,,.,11108　　　　　　301b、1c、1d,22d、2e、2f,3,.,ASBR、,.3　(1),131dASBR.,ASBRTCODSCOD63.8%83.3%.(2)3(35℃※40℃※47℃※53℃),,※(35℃※40℃)、※(47℃※53℃),※(40℃※47℃)、、COD,,40℃47℃.(3),2.038Ld,72.0%,COD(CH4COD)188.8mLg.(4)DGGE,,(35℃)(53℃).:[1]　DagueRR.Initialstudiesontheanaerobicsequencingbatchreactor[J].WaterScienceandTechnology,1992,26(9):2429-2432.[2]　IoannisS,DavidMB.Improvinganaerobicsequencingbatchreactorperformancebymodifyingoperationalparameters[J].WaterResearch,2002,36(1):363-367.[3]　AngenentLT,SungS,RaskinL.Methanogenicpopulationdynamicsduringstartupofafull-scaleanaerobicsequencingbatchreactortreatingswinewaste[J].WaterResearch,2002,36(18):4648-4654.[4]　RuizCJ,TorrijosM.Treatmentofwinerywastewaterbyananaerobicsequencingbatchreactor[J].WaterScienceandTechnology,2002,45(10):219-224.[5]　HurJM,ChangD,ChungTH.Dynamicprocessresponsetosludgethickeningbehaviorsintheanaerobicsequencingbatchreactor