:2003-02-18 :2003-03-27:(1977-),,,。冯亮,吴静,陆正禹(, 100084) :,,,,73。0.6~1.4mm;VSS/SS0.70,Vmax·CH4532ml·g-1d-1;,,,,。“”,,。:;;;:X703;S216.4 :A :1000-1166(2003)03-0012-03StudyonCultivationofThermophilicAnaerobicGranularSludge/FENGLiang,WUJing,LUZheng-yu/(DepartmentofEnvironmentalScienceandEngineering,TsinghuaUniversity,Beijing100084,P.R.China)Abstract:Thecultivationofhigheractivitythermophilicanaerobicgranularsludgewasachievedbyincreasingthetemperaturegraduallyfrommesophilicrangetothermophilicrangeinbatchexperimentsin73daysseedingwithambientanaerobicgranularsludge.Thediame-terofthethermophilccultivatedgranularsludgeismainlyfrom0.6mm~1.4mmwithVSS/SS0.70andVmax·CH4532ml·g-1d-1.Thecenterofthermophilicgranuleswerepackedwithrod-shapedbacteria,thereticularsubstanceincreasedgraduallyfromtheinteriorofgranulestotheexteriorofgranulesandcoveredentirelythewholesurfaceofgranuleslikeashell.Theshell-shapedstructureofther-mophilicgranulesfunctionedtoresisttemperture-shockanddeferdeceaseofmesophilicbacteriaandmultiplicationofthermophilicbacte-ria.Keyword:mesophilic;thermophilic;anaerobicgranularsludge;sludgeactivities [1]、[2]、[3]、[4],,。,。。1 1.1 试验设备31,250mlHZS-H(125r·min-1)。NaOHCO21000ml。。,150ml。250ml,(V)35ml。3.5V。1.2 进水水质,、COD∶N∶P150∶5∶1,,pH7.0~7.5。1.3 接种污泥12ChianBiogas2003,21(3)UASB。SS41.9g·L-1,VSS32.2g·L-1。1.4 分析项目及方法COD:TL-1ACOD;SSVSS:[5];pH:TPX-90ipH;:[6];:;:。2 2.1 中高温运行情况73,,(1~11)、(12~20)、(21~73)。2。2.1.1 (1~11)35℃。1~42.4kgCOD·m-3d-1,COD75%~80%。5~8,4.8kgCOD·m-3d-1,9~11,9.6kgCOD·m-3d-1,COD80%。2.1.2 (12~20)12,39℃,9.6kgCOD·m-3d-1,COD80%。14,45℃,4.8kgCOD·m-3d-1,15,,COD80%40%~50%。1950℃,COD40%~50%。2.1.3 (21~73)21,55℃。4.8kgCOD·m-3d-1,COD。24,COD,16.7%。COD80%。54,7.2kgCOD·m-3d-1,66,12.0kgCOD·m-3d-1,COD85%~90%,。2.2 高温颗粒污泥的性质2.2.1 ,0.4~1.4mm。,66(12.0kgCOD·m-3d-1,COD85%),0.6~1.4mm,69.1%,[7],。3。2.2.2 1。,VSS/SS。1 VSS/SS11(35℃)66(55℃)VSS(g·L-1)12.914.522.6VSS/SS0.770.790.70Vmax·CH4(ml·g-1d-1)504497532 ,13ChianBiogas2003,21(3)(,),,,。(35℃)(55℃),,,,,。12~50,。,,,,,,,,。,,。2.2.3 ,,,;。,,,“”,“”、。。([8]),,,,,,。“”,,;,,,,。,“”,。3 (1),,,73,、。2 (2)0.6~1.4mm;VSS/SS0.70,Vmax·CH4532ml·g-1d-1;,,,,。“”,,。:[1] ,,,.UASB(下转45页)14ChianBiogas2003,21(3)、,,、90°,。(1):3.2m,1.3m,1.3m×1.2m。(2):,,1.5m;1.0m,1m×0.8m0.6m。3.2 基础处理,,,,,,1.5m,C101.5m,10cm,。3.3 安砌(1),1.5m,,,2.5cm(2,,)。,5cm,,,(20cm)。(2),20cm,1.75m,70cm,。,(2,)。,,,(),M7.5。(3),,,。5,,,。(4),,,,,。,,50cm、35cm。(5),C1510cm,15cm,,。(6),,。4 (1)。、,。(2)。300mm,,C1060mm。,。(3)。1.,,;2.,5mm1∶2,;3.,2~3mm1∶1.5,;4.,2~3;5.,,。(4)。,,7,。(上接14页)[J]. ,1999,17(2):14~17.[2] .[J].,1996,14(5):8~10.[3] JCTVogelaar,EBouwhuis,AKlapwijk,HSpanijers,JBvanLier.Mesophilicandthermophilicactivatedsludgepost-treatmentofpapermillprocesswater[J].WaterRe-search,36(2002):1869~1879.[4] RichardMDinsdale,FredaRHawkes,DennisLHawkes.Comparisonofmesophicandthermophilicupflowanaerobicsludgeblanketreactorstreatinginstantcoffeeproductionwastewate[J].Wat.Res.,1996,31(1):163~169.[5] .[M].:,1998.536~543.[6] ,,.COD[J].,1985,1(4):30~36.[7] ,,.UASB[J].,2001,22(5):50~54.[8] KazuakiSyutsubo,HidekiHarada,AkiyoshiOhashi,HiroshiSuzuki.Aneffectivestart-upofthermophilicUASBreactorbyseedingmesophiliccally-growngranularsludge[J].Wat.Sci.Tech.1997,36(6-7):391~398.45ChianBiogas2003,21(3)