高效苯酚降解菌的分离与降解特性

*(,154007)=从佳木斯黑龙化工厂活性污泥中筛选到7株高效苯酚降解菌,其可以利用苯酚作为唯一的碳源和能源物质通过这7株菌在不同的温度pH值及浓度下生长和苯酚降解情况的研究,确定了此7株菌的最适生长温度25e,最适pH值712,并测得降解最大的酚浓度700mg/L该7株苯酚降解菌的降解能力研究表明:其具有较强的苯酚降解能力,在25e,pH值712装液量108mL接种量12mL摇床振荡速度110r/min及固体培养条件下培养48h,观察抗性菌生长的状态,菌落数量及测定吸光度值从而判定对苯酚的降解情况=高效;苯酚降解菌;降解特性:O625131:A:(:Dz2011-021)*(C6H6OH,PhOH),,,,,,,65e,,,,,LD2~15gMLD140mg/kg25%,0174mmol/L,10,,,(Alcaligeneseu-trophus)[2](A1calcoaceticus)[3](AlgaOchromonasdanica)[4](Rhizo-bia)[5](Pseudonomonassp.)[6](YeastTrichosporoncutaneum)[7]11111111111112(1):510g,115g,NaCl115g,1000mL,1510g,pH710~712,1@105Pa15min10-1,10-2,10-3,10-4,10-5,48h(2)[1]:KNO3410g,NaCl510g,1510g,1000mL,pH710~712,1@105Pa15min1510mL,,3,1121121131420118Agriculture&TechnologyVol131No14Aug12011#24#[8]20mL,25e48h,50mg/L100mg/L,48h5,011501200125g/L0175g/L[9]11212(1):,,5,(2):,1234567(3):¹,25e48hº[10]2211pH7,pH,pH612614616618710712714716,pH,481pH(4)pH6126146166187107127147160197601850017430159801512013860194311256212,,,,12mLpH712,010501100115012001250130013501400145015001550160016501700175g/L,110r/min25e,48h1#25#20118Vol.31No.43311311117,,015g/L0165g/L90%,0170g/L311127pH,7pH712,25e,0170g/L31113,,,312,,,,,,,,,,,,,,,,,,,,[1],,.1,2,4-[J].,2008,21(5):19-22[2],,.[M].:,1999:250-265[3]GibsonDT.Microbialdegradationofaromaticcompounds[J].Sc-ience,1968,(161):1093-1097[4]RodriguezI,LiompartMP,CelaR.Solidphaseextractionofphenols[J].JournalofChromatographyA,2000,885(1/2):291-304[5],.[J].,2000,13(1):40-44[6]FewsonCA.TheidentityofthegramnegativebacteriumNCIB8250[J].JGenMicrobiol,1967,(48):107-110[7]AndrzejW,Trochimczzuk,MichaelStreat.Highlypolarpolymeric-sorbentscharacterizationandsorptivepropertiestowardsphenolanditsderivatives[J].ReactiveandFunctionalPolymers,2001,46(3):259-271[8],,.[J].,1998,4(11):4-26[9],,.[M].:,2002:67-82:,,(1962-),,,Vol.31No.420118#26#