楼洪森,杨云龙,方明亮(,030024)[],,。,t=10~13℃、pH=7.0~7.6、ρ(O2)=2.0~2.5mg/L,,,2.5。[];;;[]X703.1[]A[]1005-829X(2013)02-0042-03ResearchonthecultivationofhighlyefficientnitrifyingbacteriatechniquesatlowtemperatureLouHongsen,YangYunlong,FangMingliang(CollegeofEnvironmentalScience&Engineering,TUT,Taiyuan030024,China)Abstract:Inordertosolvetheproblemoflowammonia-nitrogenremovingrateinwastewatertreatmentplantsinwinter,andbasedontheacclimationofordinaryactivatedsludgeatlowtemperature,thecultivationmethodofhighlyefficientnitrifyingbacteriahasbeenstudied.Experimentsshowthatwhenthetemperature=10-13℃,pH=7.0-7.6,andρ(O2)=2.0-2.5mg/L,thecultivatednitrifyingbacteriacanmaintainhigherammonia-nitrogendegradingrateatlowtemperaturebycontrollingappropriateammonia-nitrogenloadandsludgeload.Thisratereaches2.5timesofthatoftheordinaryactivatedsludgenitrifyingbacteriaammonia-nitrogenatthesametemperature.Keywords:lowtemperature;nitrifyingbacteria;ammonia-nitrogenload;sludgeload[](20070311873-01)、,。,、。、、、,〔1〕。,。11.11.1.1污泥取样A2/O,(10~13℃),。1.1.2硝化细菌培养基:(NH4)2SO41.42g,MgSO4·7H2O0.021g,K2HPO4·3H2O0.22g,NaHCO32.40g,CaCO30.05g,FeSO4·7H2O0.073g,MnSO4·4H2O0.075g,H2O1000mL。1.21.2.1影响因子、、pH、DO。〔2〕,。。〔3〕,,,。,,,,,pH。33220132IndustrialWaterTreatmentVol.33No.2Feb.,201342pH,pH7.0~8.5,pH6.5~7.5〔4〕。pH,NaHCO3,pH。(DO),DO2.0~3.0mg/L。DO0.5mg/L,;DO,,。1.2.2培养条件的确定,,12~14℃,10℃,10~13℃。pHDO,pH7.0~7.6,DO2.0~2.5mg/L。1.2.3水质分析方法〔5〕:NH4+-N;COD;MLSS。1.31.3.1培养装置,35L,26L,1。11.3.2培养方法。8h,,0.5h,6L,。2hpH、DO、,。22.1。t=10~13℃、pH=7.0~7.6、ρ(O2)=2.0~2.5mg/L,COD80mg/L,,,。2。22,,。,,,;,,。0.94g/(g·d),。,86.3mg/(g·d)。2.2,,,,,。t=10~13℃、pH=7.0~7.6、ρ(O2)=2.0~2.5mg/L,0.94g/(g·d),,,,3。3,,。COD0,,,,,,;COD,,,。2013-02,33(2),:43,,,,。1.39g/(g·d),。32.3t=10~13℃、pH=7.0~7.6、ρ(O2)=2.0~2.5mg/L,0.94g/(g·d),1.39g/(g·d),。1.0mg/(g·h),45%,2.5mg/(g·h),81%。2.5,30℃〔6〕。,,,,;,,。。2.4、(25~30℃)(10~13℃)1,pHDO7.0~7.62.0~2.5mg/L,65~75mg/L。1,,5mg/L,90%;,10mg/L,80%。,。,。13(1),,,。0.94g/(g·d)。(2),,。1.39g/(g·d),。(3)t=10~13℃、pH=7.0~7.6、ρ(O2)=2.0~2.5mg/L,0.94g/(g·d),1.39g/(g·d),,2.5,。[1].[D].:,2006.[2],,,.[J].,2000,30(9):36-38.[3].[J].,2003,13(7):103-104.[4],,.[J].,2007,30(6):50-52.[5].[M].4.:,2002:210-281.[6],,,.A/A/O-MBR[J].,2010,32(4):90-95.———————————[](1987—),。E-mail:louhongsen@sina.com。:,。E-mail:yangyunlong1956@sina.com。[]2012-11-19()/h/(mg·L-1)/(mg·L-1)/%/(mg·L-1)/(mg·L-1)/%065.43.594.6565.312.181.47867.14.193.8968.511.383.501663.51.298.1163.210.483.542464.75.291.9670.511.483.833263.62.196.7070.111.184.174064.27.188.9472.114.679.754865.14.293.5569.711.883.072013-02,33(2)44