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1241000F1。20~40℃、pH5.5~8.0、C/NKNO31.0~2.0、COD500~1000mg/L30~35℃、pH6.0~6.5C/N1.8CODF1。30%194.1mg/L、pH7.2±0.5、30℃6hF199.8%。X131.2Adoi10.3969/j.issn.1003-6504.2011.01.0101003-6504(2011)01-0041-04ScreeningofHighlyActiveAerobicDenitrifiersandItsDenitrificationPropertiesCAIChang-fengLIANGLeiDepartmentofBiochemicalEngineeringAnhuiUniversityofTechnologyandScienceWuhu241000ChinaAbstractThreestrainsofaerobicdenitrifyingbacteriawereseparatedandpurifiedfromactivatedsludgeinaerobictankofacokingwastewaterchemicalandbiologicaltreatmentstationahighlyactiveoxygendenitrifyingbacteriaF1wasobtainedbycomparisonexperimentofde-NO3-N.ThebestbiologicalremovalofNO3--Nwastemperature30~35℃pH6.0~6.5andbestC/Nratio1.8.Inthewhitesugarfortheelectiondonorandcarbonsourcenitrateasonlynitrogensourceinoculums30%initialNO3--N194.1mg/LpH(7.2±0.5)temperature30℃andafter6haerationinarowF1onNO3--Nremovalratewasupto99.8%.Keywordscokingwastewateraerobicdenitrifyingbacteriadenitrification。A2/O、A/O。。[1-2]ThiosphaerapantotrophaAlcaligenesfaecalisPseu－domonasnauticalThaureamechernichensisPseu－domonasstutzeriMicrovirgulaaerodenitrificans。Proteobacteriaα、β、γParacoccusThiobacillusAlcaligenesComamonasMicrovirgula[3][4]。。11.11.1.1COD4446mg/LNH4+-N260.7mg/L662.0mg/LCN-9.0mg/L。1.1.2《》@126.com2010-02-242010-03-17080401020021956-,ccf2005@126.com。EnvironmentalScience&Technology34120111Vol.34No.1Jan.2011,.[J].,2011,34(1):41-44.CaiChang-feng,LiangLei.Screeningofhighlyactiveaerobicdenitrifiersanditsdenitrificationproperties[J].EnvironmentalScience&Technology,2011,34(1):41-44.34。1.1.3。KYC-1102C721E。1.1.4BTB[5-6]20g/LKNO31g/LKH2PO41g/LFeCl3·6H2O0.5g/LCaCl2·2H2O0.2g/LMgSO4·7H2O1g/L8.5g/LBTB1%1mL1mol/LNaOHpH7.0~7.3121℃20min。13.2g/LKNO32g/LK2HPO40.5g/LMgSO4·7H2O0.2g/LFeSO4·7H2O0.05g/LCaC120.02g/LpH7.2±0.5121℃20min。1.21.2.1100mL250mL。30℃、160r/min24h。、1010-10。0.1mL330℃。3F1、F2F3。。1.2.2NO3--N。600nmODOD。1.2.330%F1、F2F33300mL300mL30℃、160r/min。2h1ηη/h。1.2.4250mL200mL2~330℃160r/min41600nmOD。1.2.520~40℃pH5.5~8.0C/NKNO31.0~2.0COD500~1000mg/L。1.1.4210mL300mL90mLpH7.2±0.530℃。2。22.130℃1d。1。2.21。F1。30%NO3--N194.1mg/LpH7.2±0.530℃F1、F2、F34hNO3--N90.4%、86.0%82.3%6hNO3--N99.8%、99.5%99.4%。NO3--N。F11Table1TheformsofthreebacteriaF1F2F3421NO3--NF1。2.3F1F12。F18h28h32h。2.42.4.1F1[7]。F120~40℃3。3F115～30℃NO3--N30～35℃NO3--N35℃NO3--N。F1NO3--N30~35℃。2.4.2F1pHpHpH。F1pH4。4pHF1。pH。pH6.0NO3--N95.8%pH6.5NO3--N96.4%。pH6.0~6.5F1。pH6pH6.5。pH。F1pH6.0~6.5。2.4.3C/NF1[8]。。C/NF1C/N5。5C/N1~1.8OD1.8~2.0ODC/N1.8F1C/N1.8。2.4.4CODF1433425[12]DouglasMSubhashCB,DeniseM.Assessingmodelfitbycross-validation[J].JournalofChemicalInformationandComputerSciences2003432579-586.[13].SPSS[M].2004.ZhangWen-tong.AdvancedTutorialofSPSSStatisticalAnaly－sis[M].BeijingHigherEducationPress2004.inChinese。F16。6COD500~700mg/L6hNO3--NNO3--N100%COD700mg/LNO3--NCODNO3--N、。31、pHC/NF130~35℃、pH6.0~6.5C/N1.8。2F1NO3--N194.1mg/L、pH7.2±0.5、30℃6hF1NO3--N99.8%。3F1F1。[][1]LiuJXiaW.Purificationandcharacterizationofabifunctionalenzymewithchitosanaseandcellulaseactivityfromcommercialcellulose[J].BiochemicalEngineeringJournal200630182-87.[2]DingAZFuM,ShengGY.Evidenceofaerobicdenitrifieation[J].ChinScieBull,2000,4532779-2785.[3]BoivinACherrierRSchiovonM.Acomparisonoffivepesticidesadsorptionanddesorptionprocessesinthirteencontrastingfieldssoil[J].Chemosphere200561668-676.[4]LukowT,DiekmannH.AerobicdenitrificationbyanewlyisolatedheterotrophicbacteriumstrainTLI[J].BiotochnologyLetters1997,11191157-1159.[5].[J].200528427-29.MengFan-sheng,WangYe-yao.Studyonelectrokineticremediationofsoilspollutedbychromium[J].EnvironmentalScience&Technology200528427-29.inChinese[6].[J].20017150-55.HuHong-qingHeJi-zhengLiXue-yuan.CompetitiveadsorptionofphosphateandseveralorganicacidsonAlOHxsurfaces[J].PlantNatritionandFertilizenScience20017150-55.inChinese[7].[J].200525377-81.YuAi-rongLiYouYuJi-an.DenitrificationofanewlyisolatedBacillusstrainW2anditsapplicationinaquaculture[J].JournalofMicrobiology200525377-81.inChinese[8],.[J].2007287:1548-1552.WangHong-yuMaFangSuJun-fengetal.IdentificationandcharacterizationofabacterialstrainC3capableofaerobicdenitrification[J].EnvironmentalScience2007287:1548-1552.inChinese!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!44