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WATERPURIFICATIONTECHNOLOGYVol.30,No.2,2011April25th,20111111111111111111111111[][]2008ZX07313-002。[]1982-、。010-64932999E-mail:chenyasong@bceed.net。12221.1001922.51805525~35℃、pH6.5~8.0Pseudomonassp.。10mg／L48h81.5%20mg／L50%。18d80%28d90%0.5mg／L《》GB18918—2002A。TU992.3B1009-0177201102-0019-04ScreeningandApplicationofHighEfficientPhosphate-AccumulatingOrganisms(PAOs)ChenYasong1,JinWenbiao2,YanYun2,ZhaoYongjiao2(1.BCEGEnvironmentDevelopmentCo.,Ltd.,Beijing100192,China;2.HarbinInstituteofTechnologyShenzhenGraduateSchool,Shenzhen518055,China)AbstractAhighefficientPAOswasscreenedfromBujiriversedimentinthecityofShenzhen.TheappropriategrowthconditionsofthestrainincludingpHandtemperaturewerestudied,whichrespectivelyispH6.5~8.0,temperature25~35℃.Individualbacteriummodality,physiologicalandchemicalcharactersprimarilyindicatedthatitbelongstoPseudomonassp.Whenphosphorusconcentra－tionintheenrichmentculturemediumisbelow10mg／L,thestrainhasahighphosphorusremovalcapacity,whichthephosphate-ac－cumulatingrateisabove81.5%at48h,comparedwithonly50%whenphosphorusconcentrationisupto20mg／L.Atlast,practi－calapplicationinpollutedriverwatertreatmentofthestrainshowedthatthephosphorusremovalrateswererespectivelyover80%and90%after18dand28d,afterwhichphosphorusconcentrationineffluentisbelow0.5mg／L.Keywordsbiologicalphosphorusremovalphosphate-accumulatingorganisms(PAOs)screeningpollutedriverwaterwastewatertreatmentplantWWTP201130238-41WaterPurificationTechnology[1]。[2]、、[3]。[4]3.0h。、C∶P。、、。。。11.1201130219-22WaterPurificationTechnology19--Vol.30,No.2,2011。1.25.0gMgSO4·7H2O0.5g、CaCl20.2g、NH42SO42.0g、KH2PO42~20mg、1000mL、pH7.2~7.4KH2PO42、5、8、10、15、20mg。3g、10g、NaCl5g、KH2PO415mg、15~20g、1000mL、pH7.2~7.4。1.310mL150mL30℃、150r／min2~3d8mL2~3d45、3、1、0.5mL。KH2PO42、5、8、10、15、20mg。1mL100000.2mL30℃2~3d。4。1.45、10、15、20mg／L)30℃、150r／min48、60h4000r／min20min[5]=ATP-BTP／ATP×100%。ATP———BTP———。1.55mL100mL10~40℃48h480nm。pHpH4.0~10.030℃48h480nm。1.6《》[6]。1.7－SBF-ASE－HYBFAS1。HRT=0.5hHRT=3h。5%5000L108~109cfu／LSBF-AS。22.15、4HJP07CGMCCNO2164[7]。5、10、15、20mg／L1。HJP0710mg／L。5mg／L90%。20mg／L50%。。1~6mg／LHJP07。60h。48h[9]48h。HJP07、SBF-ASSBF-AS1Fig.1FlowChartofEHYBFASProcessinPollutedRiverWaterTreatment1HJP07Tab.1ThePhosphate-AccumulatingCapacityofHJP07inDifferentPhosphorusConcentration／mg·L-148h／%60h／%593.197.21081.588.71568.373.82048.752.2.20--WATERPURIFICATIONTECHNOLOGYVol.30,No.2,2011April25th,2011、、、、、、2mm0.7~0.8μm2.0~2.8μm。1、V-P、H2S。Pseudomonassp.。2.2HJP07、pHHJP072、3。2HJP0710~40℃25~35℃30℃。23.7℃。pH5.0~10pH4.0pH6.5~8.03。2.3HJP07104cfu／L。2。DO3~4mg／L。45。0.60.50.40.30.20.110152025303540T／℃OD480nm2HJP07Fig.2EffectofDifferentTemperatureontheStrainofHJP070.70.60.50.40.30.20.10.045678910pHOD480nm3pHHJP07Fig.3EffectofDifferentpHonTheStrainofHJP072Tab.2InfluentQualityofPollutedRiverWater／mg·L-1COD135~350TN20~35TP1~6SS80~1800510152025303540／d4Fig.4VariationofPhosphorusConcentrationinInfluentandEffluent6543210TP／mg·L-10510152025303540／d5Fig.5VariationofthePhosphorusRemovalRates1.00.80.60.40.2TP／%21--Vol.30,No.2,20114、518d80%28d90%0.5mg／L《》A20d44%[8]。HJP07HJP072~4h。35、4HJP07、25~35℃、pH6.5~8.0Pseudomonassp.。HJP0710mg／L48、60h81.5%、88.7%。HJP0718d80%。28d90%0.5mg／L《》A。。HJP07。1,,.[J].,2006,24630-33.2Zhi-rongHu,M.C.Wentzel,G.A.Ekama.Anoxicgrowthofphosphate-accumulatingorganisms(PAOs)inbiologicalnutrientremovalactivatedsludgesystems[J].WaterResearch,2002,36(19):4927-4937.3.[J].2003,25(12):191-192.4.[J].2007,23(6):23-27.5.[M].(3).1989:280-286.6.[M].2001.7.[P].200710076960.6.2009.8,.PAO1-1[J].2005,21(10):13-17..。40003。、3、、3、7。4。。、、、。。、、30。。。。!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!22--