第20卷第4期2008年12月江 苏 工 业 学 院 学 报JOURNALOFJIANGSUPOLYTECHNICUNIVERSITYVol.20No.4Dec.2008:1673-9620(2008)04-0053-03* ,(,213164):,。4,,:pH9.0,700mg/L,3g/L,150r/min。,ρ(COD)=27.65mg/L,COD95.77%。46-813RE15;A572D-21,(Acetomonas.sp)(Acinetobacter.sp)。:;;:X703 :AStudyoftheTreatmentofChemicalFiberWastewaterbyHighlyActiveBacteriaTIANJing,LIEr-yang(SchoolofEnvironmentalandSafetyEngineering,JiangsuPolytechnicUniversity,Changzhou213164,China)Abstract:Thecomponentsofeffluentfromchemicalfibrefactoriesarecomplex,sochemicalfibrewastewaterisrefractoryorganicwastewater.Fourhighlyeffectivestrainstotreatchemicalfibrewastewaterwereused.Itobtainedoptimaltechnologicalconditionsbyorthogonaltest:whentheshaker′srotationspeedwas150r/min,theinoculabilitywas3g/L,theCODoftheimportwaterwasabout700mg/LandthepHwas9,CODofthecomeoutwaterwas27.65mg/LandthedegradabilityoftheCODcouldreach95.77%.Inthisfourhighlyactivebacteria,6-81and3RE15werepreservedbylaboratory;A572whichcoulddegrateethylalandD-21whichcoulddegratedioxanewereseperatedfromnature,andtheywereidentifiedasAcetomonas.spandAcinetobacter.sp.Keywords:highlyactivebacteria;chemicalfiberwastewater;technologicalconditions ,、、、,、,,[1]。、、,,1000mg/L,[2];[3],[4],,。,,*:2007-08-31:(2005-2-W-16):(1982-),,,。[5]。。1 1.1 。,、、。6-81[6](Pseudmonasputida),;3RE15[7](Pseudomonasmendocina),,,。。(1L):KH2PO45.71g,K2HPO4·3H2O1.7g,NH4Cl2.14g,10mL,pH7.0-7.2,15g。(1L):MgSO4·7H2O19.5g,Mn-SO4·H2O5g,FeSO4·7H2O5g,CaCl2·2H2O0.3g。(1L):10g,5g,NaCl5g,pH7.0-7.2,15g。1.2 COD:;:;:;、、:;OD:。《》8。1.2.1 ,,1mg/mL,1mg/mL,10mg/mL,30℃72h,1∶10005,,,,4℃。1.2.2 2%0.1g100mL,30℃,48h,。。1.2.3 ,30℃24h。50mL,30℃、120r/min24-48h。1.2.4 。。43,L9(34),4:A()、B、CCOD、DpH。1。1 Table1 FactorsandleveloforthogonalexperimentaldesignA/(r/min)B/(g/L)C/(mg/L)D18011000821102700931503120072 2.1 ,D-21A572,。2,3,4。2 Table2 TheculturecharacteristicofstrainsonLBplantA572,,,D-21,,,3 Table3 Theconfigurationcharacteristicofstrains/μmA5720.6-0.7×1.0-2.8G-D-210.9-1.0×1.5-2.5-G-4 Table4 ThephysiologicalcharacteristicofstrainsA572+-+D-21+-+ ,《》8,A572:(Acetomonas.sp);D-21:(Acinetobact-er.sp)。2.2 5。·54·江 苏 工 业 学 院 学 报 2008年2.3 6。6,COD,CDAB。6,:pH9.0,700mg/L,3g/L,150r/min。5 Table5 Degradationrateofhighlyeffectivestrainsρ/(mg/nL)/%A5721100D-211666-811[6]953RE154[8]1006 Table6 ResultsoforthogonaltestABCDCODCOD/%1A1B1C1D1379.315260.052A1B2C2D235.571694.563A1B3C3D3126.476888.774A2B1C2D3126.438480.665A2B2C3D1272.632875.796A2B3C1D2260.779372.527A3B1C3D2130.389688.428A3B2C1D3241.096474.619A3B3C2D1114.619682.47K1180.4545212.0477293.7303255.5225K2219.9502183.100392.2099142.2468K3162.0352167.2919176.4997164.670557.915044.7558201.5204113.27572.4 ,7。7 Table7 Resultsoftheoptimalprogram/hODρ(COD)/(mg/L)00.180653.8670120.200521.3472240.320268.5728360.56090.8408480.31027.6472 COD:95.77%。,COD,OD,,,,OD,,,,,,OD。3 ①D-21A572。A572(Acetomonas.sp),D-21(Acinetobacter.sp)。②,,COD,:、pH、、;:pH9.0,700mg/L,3g/L,150r/min。③,ρ(COD)27.65mg/L,COD95.77%。:[1],.[J].,2002,24(1):36-39.[2].[D].:,2003.[3]SaraLKelley,EricWAitchison,MilindDeshpande,etal.Biodegradationof1,4-dioxaneinplantedandunplantedsoil:effectofbioaugmentationwithAmycolata.sp.CB1190[J].WatRes,2001,35(16):3791-3800.[4],.[M].:,2001.[5],,.[J].,2005,25(4):295-297.[6],.PTA[J].,1996,8(2):32-34.[7],.[J].,1998,3:20-22.[8],,.[J].,1992,12(3):30-33.·55·田静等.高效菌处理化纤废水