20200412 WUYISCIENCEJOURNAL Vol.20Dec.2004:1001-4276-(2004)01-0086-07:2004-09-01:福建省教育厅科技项目资助(JA03054):郑耀通(19??),博士,副教授,研究方向:从事环境微生物与环境生物技术教学与研究工作。 qd(福建农林大学资源与环境学院,福建福州350002): ,,。,,、、、、NH3-N、。,,,。。,,。48hBOD5、NH3-N96.8%、98.7%,6.54g/L-。。: ;;;: X17,Q939 : A,,。,,--,、,。,N、P[1],,。,,。N、P[2]。,(PSB)PSB[3,4],--,,,。、、[5],PSB[6,7,8],N、PCO2DOI:10.15914/j.cnki.wykx.2004.00.019,、、、、,。。1 1.1 1.1.1 污水来源及特性 ,COD13500-18600mg/L,BOD59600-12800mg/L,T-N890-1310mg/L,T-P160-270mg/L。1.1.2 菌-藻系统组成 、、、。,。1.1.3 菌藻培养基配方与培养方法 (g/L):Ca(NO3)20.04,KH2PO40.01,MgSO40.025,Na2CO30.02,Na2SiO30.025,0.03,,pH8-10;(g/L):CH3COONa3,1,0.6,NaCl1,NH4Cl1.5,MgCl2·2H2O0.2,KH2PO40.5,K2HPO40.60,CaCl20.05,0.1,pH6.7;(g/L):10,3,4,5,NaCl5,-801,0.5,pH7.0;(g/L):200,20;pH。。(g/L),:5,10,3,0.025.,0.45,,,370C16-18h,。1.2 1.2.1 实验方法 ,,。;;。;、。1.2.2 分析测定方法,主要测定项目 1997[9]。:;:Folin-。2 2.1 、,,,,()。,,、、,。2.1.1 厌氧酸化污泥的驯化 ,5,·87·20:、。2.1.2 环境条件对酸化效率的影响 (1) 20℃、1 35℃、50℃,,(1),。,,。,,(20-35℃)。 pH2,,pH,pH,pH3.5-9,,pH5.5-6.5,pH,。2 ,1。1,,,。,,60。 1 (h)(%)1223.82439.23647.14853.66067.87275.6 pH 、、,pH,pH。,pH3。0-36pH,、、CO2、H2。(36h)pH。,pH。。·88·20 3 pH ,,4。4,24h,log10 9(cfu/L)log10 6.5,48h1.98log10。72h1.6log10。,,。、,,。,,,(4)。NH3-N,pH,NH3-NNH3,。2.2 2.2.1 菌藻初始数量比对其处理效能的影响 —:,4 。,,。-,。-,,,,-。2.2.2 环境工艺条件对菌-藻生态系统处理废水的效果影响 ·89·20:2,:。2.2.1,,。,DOCO2,,。,,,,(TCA)ATP。,,,。2 (48h) -(g/l)COD(%)BOD(%) 7.9093.798.1 6.5884.187.2 、 (),,。6.0%,NH3-N13.5%。,。-,,。,,CO2NH3-N。,NH3-N:5CO2+3H2O+NH3→C5H9O2.5N+5.25O2。NH3-N。,,。,,。,,[10,11]。,,,,,,,,,、,。,-,、,、。 -,N,,-N。3000lx,-N99.2%,1000lx,N81.3%,,CO2,,。,4,、2500~3000lx,4,,48h·90·20 ,BOD59500mg/LBOD596.8%,NH3-N98.7%,6.54g/L,。4 (48h)(mg/L)BOD5(%)NH3-N(%)244.3771.578.1486.5496.898.73 :、pH,,,。,,。,--。、、-,-,-、。--、。,-、,。,,,。DOCO2,,。-,,48h,BOD59500mg/LBOD596.8%,NH3-N98.7%,6.54g/L,。 [1].[J].,2000,26(9):287.[2],.N、P、[J].,1995,9(4):11.[3].[J].,1985,5(2):29.[4].[J].,1990,12(5):32.[5],.[J].,1998,27(3):249.[6],.[J],1999.[7],,.[J].,2002,24(3):393.[8]GoldmanJC.Outdooralgalmassculture,I.Applications[J],WaterResearch,1979,13:1.[9].()[M],,:1997.[10]HobbleJE.Competintionbetweenplanktonicbacteriaandalgaefororganicsolutes.PrimaryProductivityinAquaticEnvironments[J].UniversityofCaliforniaPress,1969.178-185.[11]CorbinEMcGriff,RossEMcKinney.Theremovalofnutrientsandorganicsbyactivatedalgae[J].Wat.Res,1972.6:1155-1164.·91·20:TREATMENTOFHIGHCONCENTRATIONORGANICWASTEWATEROFPIGGERYBYHIGHEFFICIENCYBACTERIA-ALGASYSTEMZHENGYao-tong(CollegeofResourcesandEnvironment,FAFU,Fuzhou350002)Abstract: Thepathogenicbacteriawereinactivatedandcomplicatedorganicpollutantsweredegradedtosimplesubstancebyanaerobicacidizationinadvanceforthepiggerywastewatertreatment.Theeffectsofecologicalfactorsonmetabolicrateoforganiccarbon、NH3-Nandformingproteinofbacteria-algabythebacteria-algaeecologicalsystemsarestudiedundersimulatedconditionsinthispaper.Theresultsshowedthatthemetabolicfunctionsofthebacteria-algawereaffectedbytheinitialratioofthefivesortmicroorganisms.Whentheratioofthefivegermswasmostsuitably,theabilityofthebacteria-algaesystemtoremoveammoniaandorganiccarbonwerehighest,andthoseoforganiccarbon、NH3-Nareremovedindifferentrateinlightanddarkconditions.Thelightwasmorebenefittothebacteria-algaremovingammonia,butthedarkconditionwassuitabletoreduceorganiccarbon.Usingbatchexperiment,thebacteria-algaesystemcanremovemorethan96.8%and98.7%BOD5、NH3-Nofthepiggerywastewater.Inthesametime,wecanget6.54g/Lbacteria-algaecellproteinafterwastewatertreatment.Keywords: Piggerywastewater;Bacteria-algaecosystem;Biologicaltreatment,Wasteresourcereuse·92·20