2016:2016-01-15:(201510449053);(BZXYG1401);:(1993-),,,,,()18321619205()2284098307@qq.com;,(1976-),,,,,,()15865425158()liuping0527@126.com。55820164HubeiAgriculturalSciencesVol.55No.7Apr.,201655220161HubeiAgriculturalSciencesVol.55No.1Jan.,20165520201610HubeiAgriculturalSciencesVol.55No.20Oct.,2016,,、,,[1-3]。,、[4,5]。,、,。,、,,。,,[6-10]。、、、N、P,,,,,,,(,256600):RZ14,,,a、、。,,V,。,,a0.05mg/L,CODCr50.01%,BOD573.38%,70.51%,88.59%。,RZ14,,。:;;;:X524:A:0439-8114(2016)20-5214-04DOI:10.14088/j.cnki.issn0439-8114.2016.20.013ControlEffectofEfficientAlgicidalBacteriaStrainonEutrophicWaterBodiesDINGYing-qing,FUYu-xin,XUBo,XUEShu-ping,ZHANGJiong,LIUPing(DepartmentofResourcesandEnvironment,BinzhouCollege,Binzhou256600,Shandong,China)Abstract:ForstudyofthealgicidalbacteriumRZ14stains’controleffectundernaturalconditionsofwaterblooms,Yingripoolwherebloomshadhappenedseriouslywerecarriedonin-situbioremediationbasedontheinvestigationofeutrophicationonQuanchengparklandscapewaterbody.Andchlorophyllacontent,organicmattercontent,ammonianitrogencontentandto-talphosphoruscontentweremeasuredbeforeandafterbioremediation.Theresultsshowedthatthebloomphenomenonwasse-riousinYingripool.Waterqualitywerenotuptothegenerallandscapewaterstandard(classV),belongingtohypereutro-pher.Afterbiologicalagenttreatment,bloomsdisappeared,chlorophyllacontentwaslowerthan0.05mg/L,CODCrremovalratewas50.01%,BOD5removalratereached73.38%,NH4+-Nremovalratereached70.51%,andtotalphosphorusremovalratereached88.59%.In-situexperimentsshowedthatRZ14algalinhibiteragentcouldquicklyeliminatewaterblooms.Andithadanotableeffectoneutrophicwaterbody.AlgicidalbacteriumRZ14mightbedevelopedalgicidetocontrolcyanobacteriablooms.Keywords:blooms;eutrophication;algicidalbacterium;bioremediationt20。,RZ14,(Microcystisaeruginosa)。,,。,,NP,,。,,。,,,RZ14、、,。11.1,28℃,150r/min。48h,1∶10,,,905,。26℃,800lx,12h/12h。,。,,RZ14。72h。1.2RZ14RZ14LB,16h,,FR-988,。72h,0.22μm,,5mL,50mL。,3。OD687=0.354,1.0618±0.0367mg/L,26℃,800lx,12h/12h。663、645、630、750nm,a,。a(mg/L)=11.64×(OD663-OD750)-2.16×(OD645-OD750)+0.1×(OD630-OD750)1.3610RZ14,6d,。72,,5d,3。10mg/L。,5,。1.4CODMn;CODCr;BOD5;TN-;;TP;a(Chla);(SD);DO。SPSS10.0。7700m2,1m,,,。,ETS,。,5(CODMn、Chla、TP、TN、SD),。22.1RZ14RZ141。RZ14,0.533~0.947μm×1.593~2.024μm,,。2.2RZ143d,RZ14,。5d,;7d,,,1.0096mg/L,1.5695mg/L;23d,RZ14,0.2495mg/L(2)。8d,,1RZ14(100×):5215201620min,3。CK,RZ14,,3B。,。2.3,,。(1),TP(<0.5mg/L),、、,20cm,《》(GB3838-83)V,。,,,、、,,。2.4RZ142.4.1CODCr,,,36d(4)。5d,,,,36d。54d,50.01%。2.4.2BOD5BOD5,54d73.38%(5)。,,,,,,。36d,54d,,36d。2.4.3,、(6、7)。,,,36d,,,,,54d,70.51%88.59%。2.4.4a,,1CODMnmg/L56.40BOD5mg/L20.30SDm0.10TNmg/L30.20Chlamg/L1.15×105TPmg/L0.34DOmg/L2.2020153972168192260500//d109876543210-1//mg/LCKRZ14A.CK;B.RZ143(40×)AB(P<0.05)。4CODCr053654//d6040200CODCr//mg/Labcc5BOD5053654//d20151050BOD5//mg/Labab6053654//d543210NH4-N//mg/Labab521620,a,10d,a;22d,a0.054mg/L,,a,,47d,0.045mg/L(8)。,,。3,RZ14,,,a,CODCr50.01%,BOD573.38%,70.51%,88.59%。,RZ14,。,,、[11],,(IAA)[12],,[13,14]。[15]S4,,。1∶2000,4d86.41%。。3d,,,7d,。,10d,,。。[16],96h51.4%,53.0%。、,,,,,。:[1]KEAWTAWEET,FUKAMIK,SONGSANGJINDAP,etal.Iso-lationandcharacterizationofNoctiluca-killingbacteriafromashrimpaquaculturepondinThailand[J].FishSci,2011,77(4):657-664.[2]JEONGJH,JINHJ,SOHNCH,etal.AlgicidalactivityoftheseaweedCorallinapiluliferaagainstredtidemicroalgae[J].JApplPhycol,2000,12(1):37-43.[3]ZINGONEA,ENEVOLDSENHO.Thediversityofharmfulal-galblooms:Achallengeforscienceandmanagement[J].OceanCoastManag,2000,43(8-9):725-748.[4]SUNXX,CHOIJK,KIMEK.Apreliminarystudyonthemechanismofharmfulalgalbloommitigationbyuseofsophorolipidtreatment[J].JExpMarBiolEcol,2004,304:35-49.[5]STEIDINGERKA.Are-evaluationoftoxicdinoflagellatebi-ologyandecology[J].ProgPhycolRes,1983,2:147-188.[6]FURUSAWAG,YOSHIKAWAT,YASUDAA,etal.AlgicidalactivityandglidingmotilityofSaprospirasp.SS98-5[J].CanJMicrobiol,2003,49(2):92-100.[7]MAYALIX,AZAMF.Algicidalbacteriaintheseaandtheirimpactonalgalblooms[J].JEukaryotMicrobiol,2004,51(2):139-144.[8]CHOIHJ,KIMBH,KIMJD,etal.Streptomycesneya-gawaensisasacontrolforthehazardousbiomassofMicrocystisaeruginosa(Cyanobacteria)ineutrophicfreshwaters[J].BiolCon-trol,2005,33(3):335-343.[9]SOMDEET,SUMALAIN,SOMDEEA.AnovelactinomyceteStreptomycesaurantiogriseuswithalgicidalactivityagainstthetoxiccyanobacteriumMicrocystisaeruginosa[J].JApplPhycol,2013,25(5):1587-1594.[10]SAKATAT,YOSHIKAWAT,NISHITARUMIZUS.Algicidalac-tivityandidentificationofanalgicidalsubstanceproducedbymarinePseudomonassp.C55a-2[J].FishSci,2011,77:397-402.[11]SVERCELM,SALADINB,MOORSELVANSJ,etal.Antag-onisticinteractionsbetweenfilamentousheterotrophsandthecyanobacteriumNostocmuscorum[J].JBMCResearchNotes,2011,4:357-364.[12]MAZURH,KONOPA,SYNAKR.Indole-3-aceticacidintheculturemediumoftwoaxenicgreenmicroalgae[J].JApplPhycol,2001,13(1):35-42.[13]RASHIDANKK,BIRDDF.Roleofpredatorybacteriaintheterminationofacyanobacterialbloom[J].MicrobialEcol-ogy2001,41:97-105.[14]SALLALAKJ.LysisofcyanobacteriawithFlexibactersppisolatedfromdomesticsewage[J].Microbios,1994,77(310):57-67.[15].[D].:,2011.[16].A2、[D].:,20