高效硝化细菌的富集培养与分离

2002-03-151973-ActaAgriculturaeZhejiangensis146348～3502002510640Researchonenrichmentandisolationtechniquesforhigh-efficientnitrobacteriaZHANGLing-huaKUANGZhe-shiZHANGBao-lingBio-techInstituteGuangdongAcademyofAgriculturalSciencesGuangzhou510640ChinaKeywordsnitrobacterianitritebacteriasilicagelplateenrichmentcultivationammonianitrogenQ939.11+1A1004-1524200206-0348-03111.1A2/O1.21.2.1NaCl0.3gMg-SO47H2O0.14gFeSO47H2O0.03g0.1mol/LKH2PO410mlNaNO20.5gNaHCO31.6g1L0.1MPa30min1.2.2NaNO20.5gNH42SO40.66g0.1MPa30min1.31.3.1ANaCl2.7gMgSO47H2O1.4gFeSO47H2O0.3gNaNO22.5gNaHCO316g100mlBKH2PO41.4g100mlAB910.4mlMnBCuZnCo0.1MPa30min1.3.2ANaNO2110g/LNH42SO40.1MPa30min1.42100g605ml21VV10ml10ml1mol/LNaOHpH8.2CaCl22～3h28℃1.52～3d4～52%1.61.6.1pH11.6.21Fig.1TestequipmentfornitrifyingbacteriaenrichmentNO2-7～8d61.6.3N20.5h330℃1～22～31.6.441.72522.12.1.1NaHCO3pH28～30℃pH8.0～8.2Vs30%～40%ρDO2mgL-1250mg/L300mg/L0.157mg/L0.108mg/L72.3%93.1%4531420022Fig.2Effectsofammonianitrogenconcentrationofsub-strateonnitriteconcentrationanditsremovalrate300mg/L2.1.2NaNO2NaNO2NaNO254%86%NaNO22.230℃12～32.32µmCO2Nitrosomonas2µmCO2Nitrobacter1.M.1992.2V.B.D..M.1978.3.M.1980.4.M.1995.5R.E..M.1984.6.M.1989.553