12EnvironmentalScience&Technology3212200912Vol.32No.12Dec.2009*3170005525pH518%30%322CODX703Adoi:10.3969/j.issn.1003-6504.2009.12.0151003-6504(2009)12-0063-04IsolationandCharacterizationofHighSalinityAniline-degradationBacterialStrainsLUOXin-fangLIJun-min*LUOQian-fenWUJian-jiangYUBin-binZHAOXiang-xiangWANGDan-danSchoolofLifeScienceTaizhouCollegeLinhai317000ChinaAbstract5highsalinityanilinedegradationbacterialstrainswhicharecapableofdegradationofanilineandwastewaterwereisolated.Thestrainsgrewfastwithshortdelayperiodandstableperiodaswellasoptimaltemperatureof25andpHclosetoneutral.Thetolerancetosaltwasupto18%andtowastewaterupto30%.Keywordshighsalinityanilinecontainingwastewaterdegradationbacteriaisolationcharacterization[1-3]2%~3%25%1%[4][5-7][8]-11.11NaOHpH7.02008-08-10200810162007C230522007R40G21700421986-*0576-85137067lijm@tzc.edu.cn1Table1ThepropertiesofthewastewaterCOD(mg/L)NH3(mg/L)pHP(mg/L)Cl-(mg/L)19400040.6710.6866.911.21.2.12MSANH4NO31.0gNH42SO40.5gMgSO40.632gKH2PO40.5gNaCl0.5g1000mLpH7.0~7.212130min1.2.22MSA17g500mL10%500mL1.2.3LB10g5gNaCl10g321000mLpH7.0~7.212130min1.35%7.5%10%12.5%15%1MSA10%130150r/min3048h1.41.4.11%5mLLB5%6hOD6001.4.2pH1%pHpH=5.05.56.07.07.58.08.59.09.5LB5%30150r/min48hOD6001.4.31%LB5%25303742150r/min48hOD6001.4.41%LB6%8%14%16%18%NaCl30150r/min48hOD6001.4.51%LB5%10%15%20%25%30%30150r/min48hOD6001.4.61%LB5%30150r/min24h8000r/min5minN-1-1.4.7COD1%LB5%30150r/min24h8000r/min5minCODCODCOD22.125552.255%115342436h222330h42h2.3pHpH52234pH6.0~7.024pH6.0~8.02223pH6.0~9.02.453342530374232425303742222325303742125252.51COD54415%OD1.025%30%2.655512%52.75665335.71%232224426.78%COD3COD22CODCOD2.8COD577COD970022COD9.67%24423338.62%355153225pH2515%30%3532322=24445COD22244233[][1].[J].200831468-71FangYan-fenHuangYing-pingSuJingetal.Biodegradationofbenzoicacidandanilineinwastewater[J].EnvironmentalScience&Technology200831468-71.inChinese[2].[J].,2007536748-752.HuangYing-pingFangYan-fenDuXiao-chunetal.Selectionofhighefficientstrainindegradationofphenolandaniline[J].JournalofWuhaiUniversityNaturalScienceEdition,2007536748-752.inChinese[3],.[J].,20072651738-1743.LiYanLiShe-zengRuXiu-runetal.Isolationandcharacterizationofaniline-degradationbacterialstrains[J].JournalofAgro-EnvironmentScience,20072651738-1743.inChinese[4].[J].,2001271154-56LiuZheng.Biologicaltreatmentofwastewaterwithhighsaltconcentration[J].Water&WastewaterEngineering,2001271154-56.inChinese[5].[J].200225613-14.XiongYi-dong.Experimentoftreatinganiline-containingwastewaterusingultrasonictechnique[J].EnvironmentalScience&Technology200225613-14.inChinese[6].[J].2006291219-21.KangJuanXuZe-linTaoYang-yuetal.Degradationofanilineandnicrobenzenebyionexchangemembraneelectrolysis[J].EnvironmentalScience&Technology2006291219-21.inChinese[7].-[J].200427264-65.GengChun-xiangZhangXiu-xia.Degradationofwastewatercontaininganilineandnitrobenzeneusingadsorpition-bicatalysis-hydrogenperoxide[J].EnvironmentalScience&Technology200427264-65.inChinese[8].[J].,2006262107-110.ZhangTing-tingZhangAi-liZhouJi-ti.Treatmentofhighsalinityaniline-containingwastewaterbyactivatedcarbonadsorptionseparation-biologicalregenerationprocess[J].EnvironmentalProtectionofChemicalIndustry,2006262107-110.inChinese59[7].[K].20018117-120.DongXiu-zhuCaiMiao-mo.IdentificationofCommonBacterialSystemManual[K].BeijingSciencePress20018117-120.inChinese[8]RENE.[K]..1984.BuchananREGibbonsNE.BergeysManualofDeterminativeBacteriology[M].8thed.BeijingSciencePress1984.inChinese[9].[J].200626(2)225-229.WangXiao-juanZhangRong-she.Studyonintensityofmicroorganismnitrificationanddenitrificationincontructedwetlands[J].ActaScientiaeCircumstantiae200626(2)225-229.inChinese[10].[J].200628117-19.YuZhao-xiangFengYao-yu.AstatisticalanalysisonremovalofNH3-Nandorthophosphateinwastewaterusingconstructedwetlands[J].EnvironmentalPollutionandControl200628117-19.inChinese!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!66