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,,Cl-,,,,,,,,,.,.,(up-flowanaerobicsludgeblanket,UASB)EGSB,UASB,EGSBUASB,UASB.,EGSB,,EGSB11.1EGSB,1.4m,0.12m,15.52L,15.18L.1.19m,0.16m,,0.1m,0.03m,0.08m,0.07m,EGSB,.,,,,,,,1.2,EGSB.NH4ClKH2PO4NaHCO3NaCl,NH4ClKH2PO4CNP,C:N:P=250:5:1,125:5:1.NaCl,,07500mgL-1.,1.1.3COD,5B-3(C)COD();;;.OMEGAE.Z.N.ATMMag-BindSoilDNAKitDNA,DNA.PCR341F:CCCTACACGACGCTCTTCCGATCTG(barcode)CCTACGGGNGGCWGCAG,805R:GACTGGAGTTCCTTGGCACCCGAGAATTCCAGACTACHVGGGTATCTAATCC.PCR340FCCCTAYGGGGYGCASCAG,1000RGGCCATGCACYWCYTCTC349FCCCTACACGACGCTCTTCCGATCTN(barcode)GYGCASCAGKCGMGAAW,806RGACTGGAGTTCCTTGGCACCCGAGAATTCCAGGACTACVSGGGTATCTAAT,IlluminaPCR.PCR,PCR,DNA,Qubit2.0DNADNA,1:1.()IlluminaMiSeq,,,OTURDP.Prinseq(0.20.4)FLASH(1.2.3),Mothur(1.30.1),RDPclassifier.1.4EGSBCl-,04Cl-,Cl-2000350050007500mgL-1.COD.Cl-05000mgL-1.UASB,EGSB50%.(hydraulicretentiontime,HRT)24h,R6:1,(352),pH6.8~7.2,COD3267mgL-1,COD3.267kg(m3d)-1.22.1CODEGSBCODCODpH(Cl-),COD1.1CODabcde5Cl-02000350050007500mgL-1,114dCl-10000mgL-1,,3d7500mgL-1(),Cl-7500mgL-1EGSBCOD,COD,.,COD76.2%,COD699.2mgL-1.Cl-2000mgL-1,COD,,,79.2%,COD678.25mgL-1.Cl-3500mgL-1,COD,,87.7%,COD401.9mgL-1.2500mgL-13000mgL-1Cl-COD,3500mgL-1Cl-.51d,Cl-5000mgL-1,COD,80%,COD,COD90.5%,COD307.1mgL-1;COD,COD,91d,NP.Cl-7500mgL-1,COD98.1%,,COD61.08mgL-1;COD7500mgL-1Cl-,114dCl-10000mgL-1,EGSB,,2dCl-7500mgL-1.,COD,.5000mgL-1Cl-,EGSB2COD,Cl-7500mgL-1,COD.2.2,.Cl-7500mgL-1,,.2,ab,,,0.91m3(m3d)-1.42d,Cl-3500mgL-1,,,1.19m3(m3d)-1;COD,,.Cl-5000mgL-1,,NP,,1.39m3(m3d)-1;COD.e,7500mgL-1Cl-,,,1.63m3(m3d)-1.COD,,;,2,COD.2.3,,,EGSB,.32,,,.Cl-7500mgL-110000mgL-1,0.9~0.6mm0.6mm,.,,,,,0.9~0.6mm,.,.Cl-0~7500mgL-1,2mm50%,Cl-3500mgL-1,2mm,.4,,0.9mm60mh-1,2mm.,,,,,.2.4,Cl-05000mgL-1,34562.,.Cl-0,MethanoregulaMethanothrix,50.01%32.59%,Cl-5000mgL-1,Methanobacterium(57.5%),Methanospirillum(21.9%)Methanothrix(13.91%).Methanoregula(-Euryarchaeota)(-Methanomicrobiales),H2CO2.MethanothrixMethanosaeta,,Methanosaeta.Methanobacterium(-Methanobacteriales),H2,Methanospirillum,.Methanoregula,Methanothrix,Methanobacterium,Methanospirillum.,Cl-0-Chloroflexi(23.86%),-Proteobacteria(18.99%),-Bacteroidetes(11.27%),Cl-5000mgL-1,-Bacteroidetes(29%),-Proteobacteria(23.17%)-Firmicutes(18.06%).,Longilinea(7.49%)Paludibacter(7.69%).Longilinea(-Chloroflexi)(-Anaerolineales),.Paludibacter(-Bacteroidetes)(-Porphyromonadaceae),.Cl-0,-Euryarchaeota,16SrDNA,,-EuryarchaeotaCl-5000mgL-1,.(1)COD3.267kg(m3d)-1,EGSBCl-7500mgL-1,Cl-,.Cl-7500mgL-1,COD98.1%,1.3m3(m3d)-1.,.(2)COD3.267kg(m3d)-1,0~7500mgL-1Cl-,,Cl-10000mgL-1,,.,60mh-1.(3),.Cl-05000mgL-1,Methanoregula(50.01%)Methanothrix(32.59%)Methanobacterium(57.5%),Methanospirillum(21.9%)Methanothrix(13.91%);,Longilinea(7.49%),Paludibacter(7.69%).