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302Vol.30No.22009JournalofQingdaoTechnologicalUniversity,3,(,266071):50%,A/O4,16sRNA,:nyj1nyj3Bacillus,nyj2Arthrobacter,nyj4Alcaligenes.30,pH715,430%50%,70%,,..:;16sRNA;;:X172:A:16734602(2009)02005906IdentificationandStudyonGrowthCharacteristicsofHalotolerantDomesticatedinHigh2SalinityWastewaterLIHai2yun,YUDe2shuang3,ZHANGPei2yu(SchoolofEnvironmentalScienceandEngineering,QingdaoUniversity,Qingdao266071,China)Abstract:Fourstrainsofbacteriumidentifiedassuperiorityhalotolerantwereisolatedfromsalt2resistantactivated2sludgewhichwascultivatedbyA/Oprocessinaquaticproductpro2cessingwastewatersimulatedwithseawaterratioof50%.Accordingtomorphologicalobser2vation,physiologicalandbiochemicalcharacteristicsand16sRNAsequencehomologyanaly2sis,itwasshowedthatbothnyj1andnyj3shouldbeBacillus,nyj2Arthrobacterandnyj4Alcaligenes.Theexperimentalresultsshowed,atthetemperatureof30andpH715,whentheseawaterratioswere30%and50%,thereisalittledifferencebetweenspecificgrowthratesandgenerationtimesoffourbacteria,butwhentheseawaterratiowas70%,fourbacteriawiththelowestaveragespecificgrowthrateandlongestgenerationtimeweresloweringrowth.Thisconclusionisofimportantandtheoreticalsignificanceforguidingtreatmentprojectsofhigh2salinitywastewater.Keywords:halotolerant;16sRNA;phylogenetictree;growthcharacteristic:20080922:(50678085);(J06I03);(SDYY07091):(19822),,.,.E2mail:lihy1020@126.com.3(Correspondingauthor):,.E2mail:hgdyds@263.net.0,,30.,,,[1].,[226].50%,A/O,4,,16sRNA4,,NaCl,,.11.150%A/O,TNCOD,NO-22N,,A/O,.,1mL50%,30.(50%),30.1.21.2.116sRNAUNIQ210DNAnyj1,nyj2,nyj3,nyj4DNA.16sRNAPCR27F:AGAGTTTGATCCTGGCT2CAG201492R:GGTTACCTTGTTACGACTT19..1.2.216sRNANCBIBlast(),ClustalX1.8GenBank16sRNA(MultipleAlignments),Mega3.0,(NeighborJoiningMethod),(Boostrap),1000[7].1.35mL200mL,,30,1h600nm(721),LB.,(OD),.,(1);,(2)[8].u=lnX2-lnX1t1-t2(1):u,h-1;X1t1OD;X2t2OD;t1,t2,h.G=0.693u(2):G,h;u,h-1.22.1301d,nyj1,,,1mm,,,;2d,062,:nyj2,,,1mm,,,,,,,;3d,nyj3nyj4,nyj3,,1mm,,,,;nyj4,,,1mm,,,,.1.14nyj1nyj2nyj3nyj4++++-------++--+--+---+-+-+-+++++++-+---V2P--------++++-+-+--+--+-+NaCl/%0501001505-+---++--+-+,[9][10],:nyj1(Bacillus);nyj2(Arthrobacter)(Brevibacterium);nyj3(Rubrobacter),SDN[11],DN[12];nyj4(Pseudomonas)(Al2caligenes).2.2411212416sRNA16sRNA(124),:nyj1nyj3Bacil2lus,nyj1BacilluscereusstrainD62Bacil2lussp.cp2h63Bacillussp.cp2h45Bacillussp.B232Ydz2hnBacillussp.B75Ydz2zzBacillussp.Z7Bacillussp.Cobs2Tis11Bacilluscereusstrain42112Bacillussp.cp2h36,99%99%99%99%100%99%99%99%99%,nyj3Bacillussp.VAN14,99%;nyj2Arthrobacter,Arthrobacterproto2phormiae,98%;nyj4Alcaligenes,Alcaligenessp.LJ2F,99%.16sRNA.16302.3430,pH7.54,5-8430%50%70%,:430%50%70%,nyj1nyj2nyj31h,nyj43h,,,414h,,;24h,(OD),nyj1nyj2nyj350%30%,nyj4;,,450%,30%70%,.,4,2.262,:24/%/h-1/minnyj1300.80451500.80351700.78352nyj2300.63265500.58370700.49883nyj3300.58870500.55475700.52379nyj4300.42199500.403103700.3801092:430%,,50%,70%,.nyj130%70%50min,,nyj1;nyj26580min,,nyj2;nyj375min,,nyj4;nyj4100min,,nyj4.,nyj1,3,nyj2,3.3(1),nyj1nyj3(Bacillus),nyj2(Arthrobacter),nyj4(Alcaligenes),nyj2,nyj3,nyj4.(2)450%,30%70%,,,30%50%,,70%,,;,.(References):[1],.[J].,1998,11(4):1992203.YANGJian,GUOChang2jiang.EffectsofHighSodiumChlorideandSodiumSulfateonActivatedSludgeTreatment[J].PollutionControlTechnology,1998,11(4):1992203.[2]HamodaMF,Al2AtlarMS.EffectsofHighSodiumChlorideConcentrationonActivatedSludgeTreatment[J].WatSciTech,1995,31(9):61272.[3]KargiF,DincerAR.EffectofSaltConcentrationonBiologicalTreatmentofSalineWastewaterbyFed2BatchOperation[J].EnzymeandMicrobialTechnology,1996,19:5292537.[4].[J].,2004,24():2092211.LIUZheng.BiologicalTreatmentTechnologyofHigh2SaltConcentrationWastewater[J].EnvironmentalProtectionofChemicalIndus2try,2004,24(Suppl.):2092211.[5],,,.[J].,2002,22(6):5462550.HEJian,LIShun2peng,CUIZhong2li,etal.IndustrialHypersalineWastewaterBiochemicalTreatmentofSalt2TolerantSludgeAccli2mationandItsMechanisms[J].ChinaEnvironmentalScience,2002,22(6):5462550.[6]PanswadT,ChadarutA.ImpactofHighChlorideWastewateronanAnaerobic/Anoxic/AerobicProcesswithandwithoutInoculationofChlorideAcclimatedSeeds[J].WatRes,1999,33(5):116521172.[7],,,.I[J].,2005,26(4):32237.3630CHENCui2zhen,FANGHai,ZHANGXiao2jun,etal.BiologicalPropertiesandPhylogeneticAnalysisofAcinetobacterJuniiMorpho2varI[J].MarineFisheriesResearch,2005,26(4):32237.[8]PhilippG.[K].,.:,1989.PhilippG.ManualofMethodtoOrdinaryBateriology[K].Translator:MicrobiologicalStaffRoomofXiamenUniversity.Xiamen:Xia2menUniversityPress,1989.[9]RE,NE.[K].8.,.:,1984.BuchananRE,GibbonsNE.BergeysManualofDeterminativeBacteriology[K].8thed.Translator:TheTranslateandEditGroupofBergeysManualofDeterminativeBacteriologyofMicrobiologicalInstituteofChineseAcademyofScience.Beijing:SciencePress,1984.[10],.[K].:,2001.LIXiu2zhu,CAIMiao2ying.TheManualofSystematicMethodsofDeterminativeBacterial[K].Beijing:SciencePress,2001.[11],,,.2[J].,2005,11(2):2262228.ZHANGGuang2ya,FANGBai2shan,MINHang,etal.IdentificationandPhylogeneticAnalysisofanIsolatedAerobicSimultaneousNitrifier2Denitrifier[J].Chine