2017:2016-12-01:(BE2016653);(WS201521):(1991-),,,,,()13915046002()463961464@qq.com;,(1968-),,,,,()zwy@cczu.edu.cn。551320167HubeiAgriculturalSciencesVol.55No.10May.,201655220161HubeiAgriculturalSciencesVol.55No.1Jan.,2016561320177HubeiAgriculturalSciencesVol.56No.13Jul.,2017,、,(),(COD)、(TP)、(TN)、、,(SBR、BAF),TN、TP。,A/O,Kornaros[1]COD,A/O,。Nah[2]-A/O1,1,2,1,1,1,1,1(1.,213164;2.,213164):1B8,A/O,(TN)(TP),B8A/O,B8TN。,14dB8A/O,TP73.03%,TP57.31%,TN70.72%,TN61.17%,B8A/O。A/OTNModifiedStover-Kincannon。:;A/O;;:X703:A:0439-8114(2017)13-2446-05DOI:10.14088/j.cnki.issn0439-8114.2017.13.012ResearchonTreatmentofRuralDomesticWastewaterwithTechnicalProcessofMultipleStagesA/OwithoutRefluxandEnhancementbyFunctionalBacteriaHUANGBin1,ZHOUXin-cheng1,2,CHENDong1,GEQiu-fan1,CHENZe-hui1,DONGXiao-na1,ZHANGWen-yi1(1.SchoolofEnvironmental&SafetyEngineering,ChangzhouUniversity,Changzhou213164,Jiangsu,China;2.ChangzhouShuizhongtianEcologicalGardenEngineeringLimitedCompany,Changzhou213164,Jiangsu,China)Abstract:Inthisstudy,ahigh-effectivedenitrificationbacteriawasisolatedfromthesewagesludgeandnamedasB8,whichwasusedtoaddedinthereactorofmultiplestageA/Oprocesswithoutrefluxforimprovingtheremoveoftotalnitro-genandphosphors.TheeffectandimprovementmechanismoftheremoveoftotalnitrogenandphosphorsbymultiplestagesA/Oprocesswithoutrefluxwasexamined,anddegradationkineticmodeloftotalnitrogenwasinvestigated.Theresultsshowedthat,whenB8wascontinuouslyaddedintothereactorofmultiplestageA/Oprocesswithoutrefluxfor14days,theaverageremoveratesoftotalnitrogenandphosphorswere70.72%and73.03%,respectively,whichwerehigherthan61.17%and57.31%correspondingtothatwithouttheadditionofB8.ThisresultindicatedthattheadditionofB8couldenhancere-moverateoftotalnitrogenandphosphorswithmultiplestageA/Oprocesswithoutreflux.TheremovekineticmodeloftotalnitrogenfittedwiththemodelofModifiedStover-Kincannon.Keywords:denitrificationbacteria;multiplestageA/Oprocess;domesticwastewater;denitrificationanddephosphorization13(C/N)(MLSS)。Murat[3],。,、、A/O[4],B8,A/O,、、(NH4+-N),A/OB8,A/O。11.11.1.1AOA/O1,A、B,A(CK),B。/,,(DO),,[5,6],。2.4、3.7、2.4、3.7、2.4L,,,,-,“-”[7],,,[7]。1.1.2、1B8,16SrDNA,(Pseudomonasputidasp.),[8]。B8pH6.5PAM,30℃、120r/min20~24h,Poly-PB8(6.25×107~8.55×107CFU/mL)[9]。1.2CODCr251.31~573.33mg/L,TP1.83~3.54mg/L,TN12.54~18.69mg/L,NH4+-N6.84~11.06mg/L,NO3--N3.69~5.94mg/L。1.3AO、,。1。、,。30d,100mL,2,14d,6h。1.4《()》[10]。22.1COD,44dA/OCODcr2。ACODcr345.74mg/L,73.91mg/L,79.25%;B1.;2.;3.;4.;5.;6.;7.;8.;9.;10.;11.;12.1A/O2B812345B8//109CFU6.22~8.556.25~8.556.22~8.556.22~8.556.22~8.55//d22226189101112L2.43.72.43.72.4m3/h1.26~1.541.20~1.471.17~1.471.11~1.361.08~1.32m3/h1.41.31.31.21.2h1.702.771.852.992.00:-A/O24472017CODcr393.97mg/L,72.78mg/L,81.05%。,B8CODcr。A/OCODcr75%~85%,80.04%,CODcr50~100mg/L,《》(GB18918-2002)B。2.2TP325~30℃,0.312m3/(m2·h)ABTP。3,BB8A/OTP,ATP1.117mg/L,57.31%;BTP0.754mg/L,73.03%,ATP27.43%。,B8、、[11];B8,[12];B8A/O。2.3TN425~30℃,0.312m3/(m2·h)ABTN。4,ATN15.83mg/L,,6.12mg/L,61.17%;B,3.94~5.15mg/L,70.72%,A15.61%。,TN,TN,TN。A/OAO,B8A/O。2.4NH4+-N525~30℃,0.312m3/(m2·h)ABNH4+-N。5,ANH4+-N,51.32%,95.44%;BNH4+-N,0.377mg/L,95.38%,。2.5NO3--N625~30℃,0.312m3/(m2·h)ABNO3--N2CODcr051015202530//d8007006005004003002001000CODcr//mg/L100908070605030//%(A)(CK)2468101214//d8007006005004003002001000CODcr//mg/L100908070605030//%(B)3TP051015202530//d4.03.53.02.52.01.51.00.50.0TP//mg/L10090807060504030//%(A)(CK)//%(B)2468101214//d4.03.53.02.52.01.51.00.50.0TP//mg/L100908070605040302448134TN。NO3-N2、N2O[13]。,30d,NO3--N4.61mg/L,1.86mg/L,59.37%;1.55mg/L,71.69%,20.75%。NO3--N,,A,,,,NO3--N。2.6ModifiedStover-KincannonModifiedStover-Kincannon[14,15],[16],(1)(2)。dsdt=QV(Si-Se)(1)dsdt=Umax(QSi/V)KB+(QSi/V)(2)(1)(2):dsdt!-1=VQ(Si-Se)=KBUmaxVQSi+1Umax(3)051015202530//d2220181614121086420TN//mg/L//%(A)(CK)2468101214//d//%(B)100908070605040302220181614121086420TN//mg/L100908070605040305NH4+-N051015202530//d121086420NH4+-N//mg/L//%(A)(CK)2468101214//d//%(B)100806040NH4+-N//mg/L100908070605040301210864206NO3--N051015202530//d654321NO3--N//mg/L//%(A)(CK)2468101214//d//%(B)10090807060504030654321NO3--N//mg/L10090807060504030:-A/O24492017,KB,kg/(m3·d);Q,L/d;V,L;SiSe,kg/m3;ds/dt,kg/m;Umax,kg/(m3·d)。VQ(Si-Se)y、VQSix[17],4,7,AOModifiedStover-Kincannon。7,Umax=0.184kg/(m3·d),KB=0.333kg/(m3·d),0.9928。0.139kg/(m3·d),Umax75.54%。Umax、KB(3),:Se=Si-Si1.81-5.42(QSi/V)(4):E=11.81-5.42(QSi/V)(5)2.7A/OA/O,,,,,[4]。,,[18]。A/O,,,DO[19],,,300%,100%,80%[19]。A/O,,。,AO(HRT)5h,,[20],,A/O。BTN72.33%,,TN,。3、,A/O,。,14dB8A/O,BTP、TN、NH4+-N、NO3--N73.03%、70.72%、95.38%、71.69%,A57.31%、61.17%、71.34%、59.37%。B8A/O,ModifiedStover-KincannonSe=Si-Si1.81-5.42(QSi/V)。:[1]KORNAROSM,MARAZIOTIC,LYBERATOSG.Apilotscalestudyofasequencingbatchreactortreatingmunicipalwastewa-teroperatedviatheUP-PNDprocess[J].WaterScienceandTechnology,2008,58(2):435-438.[2]NAHYM,AHNKH,YEOMIT.Nitrogenremovalinhouse-holdwastewatertreatmentusinganintermittentlyaeratedmem-branebioreactor[J].EnvironmentalTechnology,2000,21(1):107-114.[3]MURATHOCAOGLUS,ATASOYE,BABANA,etal.Nitrogenremovalperformanceofintermit