共固定化微生物对养殖水体脱氮的研究杜刚

()30(4):550～553,2007JournalofShanxiUniversity(Nat.Sci.Ed.)　　:0253-2395(2007)04-550-04共固定化微生物对养殖水体脱氮的研究杜　刚1,王京伟2*(1.,710302;2.,030006)　:将光合细菌、枯草芽胞杆菌、放线菌、硝化细菌和反硝化细菌共同固定在同一个载体内,构建成简单的人工微生态系统研究其对养殖水体的脱氮作用.结果表明,微生物共固定化对养殖水体脱氮效果优良,对氨氮、硝基氮、亚硝基氮的降解率可达85.0%、79.3%、87.3%.:固定化微生物;氨氮;硝酸盐氮;亚硝酸盐氮;生物脱氮:X52　　　:A,,,.,,.,.,[1～3].,、,,,,[4,5].,,.1　1.1　:(Rhodopseudomonaspalustris)、(Bacillussubtilis);:(Streptomycesmicroflavus).;:(Nitrobactrwinogradskyi);:(Alcaligenesfaecalis).1.2　30℃5d,.、、、37℃24h～36h,,37℃36h～48h.1.3　[6～9]80℃10%()(PVA)3%()(CA),,pH7.4,,,.,,、、.:2006-12-20;:2007-09-10　　:(051039-4)　　:　(1975-),,,,.:E-mail:[e-mail]:10.13451/j.cnki.shanxi.univ(nat.sci.).2007.04.0301.4　[10]1,、、、4..,,,.1　Fig.1　Reactionsystem1.5　,,1d.200656,3,3.1;2(109);3,2.1.6　,,.,,,0.2mol/L,20min.,,.1.7　[11];N-(1-)-;.2　2.1　2,,,5mm4mm3mm,.,.,,,4mm.2.2　.3(,0.12mg/L,0.20mg/L,0.38mg/L),4mm.(3).2　Fig.2　Effectofimmobilizedmembraneofdifferentsizesongrowthofbacteria3　Fig.3　Effectsofinitialbacteriadensityongrowthofbacteria　　3,,,.4,2,.,.551　　　　　　　　　　　　　　　　:,.,,,,,()0.20mg/L.4　Fig.4　Effectofrotationalspeedofbladeongrowthofbacteria2.3　,,,.,,,.,.,.2.4　4,,,0.90r/min,,5,,,,,,,.30r/min60r/min,.,,,30r/min.2.5　3,.、、5、6、7.5　Fig.5　Degradationofammonianitrogenbyimmobilizedmembrane6　Fig.6　Degradationofnitritenitrogenbyimmobilizedmembrane7　Fig.7　Degradationofnitratenitrogenbyimmobilizedmembrane　　5、6、7,3、、,85.0%、87.3%、79.3%;1(),;2、、55.6%、48.3%、49.7%,3.2,,2,,.3,35,2.　　　　　　　　　　　3　,,,,,,、、,,552()　　　　　　　　　　　　　　　30(4)　2007　,4mm,0.20mg/L,、、85.0%、87.3%、79.3%,.,,.:[1]　,,　,.[J].,2005,12(3):198-200.[2]　UKEnvironmentalAgency.EnvironmentalISSUESSeries-aquaticEutrophicationinEnglandandWales[R].UKEnvir-onmentalAgencyConsultativeReport,December1998.[3]　.[J].,2002,18(1):42-45.[4]　　,.[J].,2005,25(4):27-29.[5]　,,,.[J].,2005,24(1):68-70.[6]　,,.[J].2006,12(2):251-254.[7]　　.[J].,2005,21(1):72-74.[8]　　,.[J].,1997,18(1):18-23.[9]　,,.Cd[J].,2005,14(1):17-21.[10]　BALAJINSASTRY,AURELIOA.DELOSREYESJ,KELLYARUSCH.NitrificationPerformanceofaBubble-washedBeadFilterforCombinedSolidsRemovalandBiologicalFiltrationinaRecirculatingAquacultureSystem[J].AquaculturalEngineering,1999,19:105-117.[11]　,,,.[M].:,1989.StudyontheDenitrificationofAquicultureWaterBodybyImmobilizedMicrobesDUGang1,WANGJing-wei2(1.DepartmentofHeatandChemicalEngineering,ShanxiGuofangInstituteofTechnology,Xi'an710302,China;2.InstituteofModernChemisty,ShanxiUniversity,Taiyuan030006,China)Abstract:Theeffectsofasimplemicroecosystemofimmobilizedmicrobesincludingphotosyntheticbacteria,Bacillussubtilis,actinomyces,nitrobacteriaanddenitrifyingbacteriaonthedenitrificationofaquiculturewaterbodywerestudied.Theresultsindicatedaftertheaquicuhurewaterwastreatedwithimmobilizedmicrobes,theconcentrationsofammonianitrogen,nitratenitrogenandnitritenitrogeninaquaticecosystemobviouslydecreasedby85.0%,79.3%and87.3%.Keywords:immobilizedmicrobe;ammonianitrogen;nitratenitrogen;nitritenitrogen;biodenitrification553　　　　　　　　　　　　　　　　: