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J251)尹华余莉萍彭辉叶锦韶张娜(,,510632)AzotobacterJ25.,,AzotobacterJ25,,,98%.,.,,,CODCr,SS,667%,983%,937%.,,.,,[14].,,,.AzotobacterJ25,.111:10gl-1,20gl-1,KH2PO41gl-1,MgSO47H2O05gl-1,pH70.:()(V/V),10gl-1,pH70..,CODCr528963mgl-1,BOD538431mgl-1,4931mgl-1,SS22425mgl-1,pH10.:80,,.250ml50ml,,34,150rmin-130h,.12150ml95ml4gl-1005ml5ml200298.1)(980897)(939711).226200311ENVIRONMENTALCHEMISTRYVol.22,No.6November200310gl-1CaCl2,,(1min,2min),10min,721550nm.,,:=(A-B)/A100%,A:550nm,B:550nm.13AzotobacterJ25,.CODCr,SS,SS,[5].221J25,,,.1,[6],AzotobacterJ25.1Table1Strainidentification-+VP++++-++H2S-++++-+22(0100%),(10gl-1),,34,150rmin-130h,,,2.2Table2Effectofwastewaterconcentrationonflocculatingactivity(V/V)/%01020304050607010()/ml-108928447765614793930772/%090487086475474860006945836:J252,,,10%30%,,.70%,,.,,.,,.,,,0%20%,1.:25%,,.,.125%,,.,125%,.,,AzotobacterJ25,98%.1Fig1Effectofwasterwaterconcentrationonflocculatingactivity23AzotobacterJ25125%AzotobacterJ25,pH,2.2J25pHFig2GrowthcurveofstrainJ25,flocculatingactivityandpHwithtime:,,;,,,,58422;pH,.AzotobacterJ25,20h;,32h.,20h,,,,..,.,95%,15%,,.243.3,AzotobacterJ25,100ml005ml,968%.:,.,.25pHpH,pH,.4,pH,,.,;,,,,.3Fig3Effectofthevolumeofculturingliquidonflocculatingactivity4pHFig4EffectofwastewaterpHonflocculatingactivity2620min,,5.5,30,;3070,60;70,.,.,(5856:J25),,;,,,,.,,,,,,,.5Fig5Effectoftemperatureonflocculatingactivity27AzotobacterJ25,.,1mll-1(3).:,,,,.:,,.3J25Table3ThetreatmenteffectofwastewaterCODCr(mgl-1)SS(mgl-1)()CODCr(mgl-1)SS(mgl-1)()CODcr(%)SS(%)(%)30005258253000213929310912731281484227555238225703646431531612157342066798393752896322425637522403182318325766337132006623601501327439453398147003(1)AzotobacterJ25,,125%.(2):AzotobacterJ25,58622.05mll-1,,,.,.60,.(3)AzotobacterJ25,,1mll-1,CODCr,SS667%,983%937%,.[1]ShihIL,VanYT,YehLCetal.,ProductionofaBiopolymerFlocculantfromBacilluslicheniformisandItsFlocculationProperties.BioresourceTechnology,2001,78(3)267_272[2]SalehizadehH,VossouhiM,AlemzadehI,SomeInvestigationsonBioflocculantProductionBacteria.BiochemicalEngineeringJournal.2000,5(1)3944[3]SalehizadehH,ShojaosadatiSA,ExtracellularBiopolymericFlocculantsRecentTrendsandBiotechnologicalImportance.BiotechnologyAdvances,2001,19(5)371385[4]RyuichiroK,YasuhiroN,MicrobialFlocculationofWasteLiquidsandOilEmulsionbyaBioflocculantfromAlcaligenesLatus.Agric.Boil.Chem.,1991,55(4)11271129[5],,,.:,1996[6]RE,NE,..:,1984STUDYONFLOCCULANTPRODUCEDFROMAZOTOBACTERJ25BYUSINGGLUTAMICACIDWASTEWATERYINHuaYULipingPENGHuiYEJinshaoZHANGNa(DepartmentofEnvironmentalEngineering,JinanUniversity,Guangzhou,510632)ABSTRACTFlocculationcharacteristicsofbioflocculantproducedbyAzotobacterJ25inglutamicacidwastewaterwerestudied.Afterpretreatmentandadditionofglucose,glutamicacidwastewaterwasusedtocultivateAzotobacterJ25.Theexperimentalresultsshowedthattheextracellularflocculantproducedbythismeanshadover98%offlocculationactivity.ThesuitablepHvalueforthebioflocculanttreatingKaolinclaysuspensionwasweakalkaline.Thewastewaterpurificationexperimentsdemonstratedthatthebioflocculanthadgoodpurifyingeffectinthetreatmentofvariouswastewaters.Especiallyinthetreatmentofpetrochemicalwastewater,thesatisfactoryeffectwasachievedwiththeremovalratioofCODCr,SSandchromawere667%,983%and937%,respectively.Keywords:microorganism,flocculant,glutamicacidwastewater.5876:J25