固定化Ralstoniamet省略ransCH34降解苯酚的研究吴志国

RalstoniametalliduransCH34*吴志国1　王艳敏1　邢志华2　武晓炜2　宋水山3**(　　300130)1(　　052160)2　(　　050051)3:RalstoniametalliduransCH34。,,。,R.metalliduransCH34;;(,),R.metalliduransCH34。:,,,:Q789　　:A　　:0253-2654(2005)04-0031-06　　*:3　　3　2**　Tel:[phone],E-mail:[e-mail]:2004-09-17,:2004-11-04StudyonDegradingPhenolbyImmobilizedRalstoniametalliduransCH34*WUZhi-Guo1　WANGYan-Min1　XINGZhi-Hua2　WUXiao-Wei2　SONGShui-Shan3**(DepartmentofBiotechnologyEngineering,HebeiUniversityofTechnology,Tianjin300130)1(NorthChinaPharmaceutialHuashengCo.,Ltd.,Shijiazhuang052160)2(LaboratoryofEnvironmentalEngineering,HebeiInstituteofBiology,Shijiazhuang050051)3Abstract:ThesubjectofthisstudyistoimprovetheabilityofRalstoniametalliduransCH34,aphenol-degrad-ingstrainwithanti-heavymetalfeaturebyimmobilization.Theoptimalconditionsforimmobilizingbacterialcellswereobtainedwithcrossactexperiment.Thephenol-degradingefficiencyofimmobilizedRalstoniametalliduransCH34wasinvestigated.Resultsshowthatphenol-degradingefficiencyofimmobilizedcellsisobviouslysuperiortothatofthefreecells;Theabilityofitsresistancetoheavymetalhavebeengreatlyimproved;Whenadditionalcarbonsourceswereaddedinthesimulatedwastewater,theimmobilizedR.metalliduransCH34wereaffectedlessthanthefreecells.Keywords:Anti-heavymetal,Immobilizedcells,Crossact,Additionalcarbonsources。,,。,,。RalstoniametalliduransCH34,,[1]。,R.metalliduransCH34。,,,。31　200532(4)　　　　　　　　　　　　DOI:牨牥牨牫牫牬牬牤jmicrobiolchina牪牥牥牭牥牬牥牥牱1　1.1　1.1.1　:RalstoniametalliduransCH34AlexanderSteinbüchel,。1.1.2　:、4-、(8%)AR,、、、、、、CP。1.1.3　:M9。M9:Na2HPO412H2O6g,KH2PO43g,NaCl0.5g,NH4Cl1g,MgSO27H2O0.492g,CaCl20.0111g,300mg,1L()。1.2　1.2.1　:4-[2]。1.2.2　:,。:(5,000r/min15min)M9(0.3g/10mL),,,,、,18h[3],,。1.2.3　:20mL100mL,30℃,250r/min。2　2.1　Ca2+pH,,。,Ca2+pH,,1。:CaCl2pH6,Ca2+,。,,,;Ca2+,,,。4-,,Ca2+,。M9pH6Ca2+。2.2　,、、。,[4]L9(34)(),。18h,2。32　　　　　　　　　　　　200532(4)1　Ca2+pHCa2+pH≤67～11≤0.001mol/L+-≥0.01mol/L+++:+,++,-、2　ABC12%3%1∶2523%4%2∶2534%5%4∶25:ACaCl2,B,C(/)　　2,9,20mL,4h,3。3　　　ABCi11111104.4622122287.4773133330.5984212345.21352231136.45662312111.96673132209.52983213155.02093321169.635k1j253.768390.434402.679441.783k2j324.866410.184333.556440.203k3j565.415343.430407.814262.06K1j74.047119.866123.816136.851K2j97.747126.186100.907136.456K3j177.930104.067125.39676.812Rj88.2676.5048.87444.423A1B2C3Yi:(mg/L)A1B2C3　　:(1)4h,90%。(2),,A1B2C3,9,9(A1B3C3),4。4　t(h)02468303.654300.377256.928162.5250A1B2C3A1B3C31307.559197.67981.947002299.998157.3900001305.325196.88980.763002301.695126.581000:mg/L33　200532(4)　　　　　　　　　　　　,,A1B3C3,A1B3C3,:4.8g/L(/),2%,5%。2.3　,。,,。,20d,,;,30d,,。,,Ca2+。30d6h,32～3h。2.4　19:2h1　1,2,3,45,6,7,89,3,,。3(),,,,,。Monod,C,,:dlnC/dt=-k[5]。,,5。,。53|k|,。5　R21lnC=-0.2611t+5.68530.99932lnC=-0.3014t+5.70530.99353lnC=-0.5644t+5.85470.93184lnC=-0.4688t+5.79960.958134　　　　　　　　　　　　200532(4)55lnC=-0.1872t+5.64990.99536lnC=-0.2360t+5.66650.99977lnC=-0.0851t+5.66220.99688lnC=-0.1621t+5.65190.95659lnC=-0.1369t+5.64680.9552　500mg/L,500mg/L,500mg/L,300mg/L2.5　300mg/L500mg/L,,2。R.metalliduransCH34,,,。1,000mg/L,R.metalliduransCH3460h,R.metalliduransCH34。2.6　R.metalliduransCH34,,Hg2+,10μmol/L;Co2+、Ni2+,2mmol/L[6]。:。Co2+、Ni2+32h、48h,Hg2+80h;Co2+、Ni2+11h、13h,Hg2+31h。R.metalliduransCH34。2.7　R.metalliduransCH34R.metalliduransCH343,R.metalliduransCH34,4。3　□OD600,■5h4　,+,[phone](4)　　　　　　　　　　　　　　43,R.metalliduransCH34R.metalliduransCH34,。3　(1)R.metalliduransCH34,,:4.8g/L;2%;5%。。(2)R.metalliduransCH34,、、、。[1],,,.,2003,20(4):245～251,262.[2]《》..:,1997.408～410.[3],　,.,1993,14(2):11～14.[4]..:,1995.214～239.[5],　,.,2001,1(4):439～441.[6]DielsL,SadoukA,MargeayM.AGeneticApproachToxicolEnvironChem,1989,23:78～89.··《》,。,。,197530,,,5～15、,;(Fe3O4),500／～1000／;,3.8%,0.013%,100;(),。、、,,。,,“”,“”,。“”(),“”()。,,,。,,,,,,,,。,、“”,。　36　　　　　　　　　　　　200532(4)