固定化氨氧化细菌短程硝化特性研究赖鼎东

:,,1981,,。#。＊“973”(No.2008CB418003);(No.2006BAJ08B01-02);(No.JS2004ZB02);(No.WTWER0707)。固定化氨氧化细菌短程硝化特性研究＊赖鼎东1　李正魁1,2#　张晓姣1　杨竹攸1,2　石鲁娜1(1.,,　210093;2.,　210093)　　　,,、pH、。,、pH30℃8.5;/NH+4-N()=6.75,87.5%;,,4.03mg/L。　　　　　Characterizationofshort-cutnitrificationusingimmobilizedammonia-oxidizingbacteria　LaiDingdong1,LiZhengkui1,2,ZhangXiaojiao1,YangZhuyou1,2,ShiLuna1.(1.StateKeyLaboratoryofPollutantControlandRe-sourcesReuse,SchoolofEnvironment,NanjingUniversity,NanjingJiangsu210093;2.EngineeringCenterofWa-terTreatmentandEnvironmentRemediation,MinistryofEducation,NanjingJiangsu210093)Abstract:　Ammonia-oxidizingbacteria(Pseudomonassp.)wereimmobilizedbyadsorptionontheporouscopol-ymercarriers.Theeffectsoftemperature,pH,alkalinityandDOconcentrationonshort-cutnitrificationmediatedbytheattachedbacteriaweredetermined.Whenthetreatmentwasperformedat30℃,pHof8.5,andthealkalinity/NH+4-Nof6.75,87.5%ofammoniawasoxidizedtoNO-2-N.TheDOlevelaffectedtheammoniaoxidationratebuthadnoeffectontheshort-cutnitrificationefficiency;thebestDOforthesystemwas4.03mg/L.Keywords:　immobilized;ammonia-oxidizingbacteria;short-cutnitrification　　、、,。,,NO-2-NNO-2-N[1,2]。,、、pH、(FA),,,,DelftSHAR-ON[3]135-142,[4-6]。,[7],。　　,,、pH、,,NO-2-N,。1　1.1　化学试剂　　β-(2-Hydrosyethylmethyl-acrylate,HEMA)、(2-Hydroxyethyl-acrylate,HEA)Fluka,。。1.2　实验仪器　　pHS-3CpH、T52N—、JBPJ608、THZ-82A、60Co-γ。1.3　氨氧化细菌及其培养基　　,,、1NH+4-N。16SrRNA,(Pseudomonassp.)。:(NH4)2SO42.0g、K2HPO4·13·　DOI:10.15985/j.cnki.1001-3865.2008.11.0080.5g、MgSO40.5g、FeSO4·7H2O0.4g、NaCl0.5g、Na2CO31.0g,1000mL,pH7.2。1.4　载体的制备　　β-、,,-78℃,1×104Gy60Co-γ[8]。1.5　菌种的固定化与富集　　5mm×5mm×5mm,,,。500mL,,。1.6　实验装置　　1。,1.7L(8cm、50cm)((30±1)℃,),10%()。,(300mL/min,),,NH+4-N、NO-2-NNO-3-N。1　Fig.1　Experimentalapparatus1—;2—;3—;4—;5—;6—;7—1.7　实验废水　　,(NH4)2SO4,NH+4-N10gNaHCO3NaHCO3(,CaCO3,),、,pH8.0(pH)。1.8　测定项目与方法NH+4-N;NO-2-NN-(1-)-;NO-3-N;[9];pHpHS-3CpH;JBPJ608。1.9　计算公式　　(η1,mg/(L·d))(η2,%):η1=(cNH+4-N1-cNH+4-N2)/t(1)η2=cNO-2-N2/cNO-X-N2×100%(2):cNH+4-N1NH+4-N,mg/L;cNH+4-N2NH+4-N,mg/L;cNO-2-N2NO-2-N,mg/L;cNO-X-N2NO-X-N,mg/L;t,d。2　2.1　温度对固定化氨氧化细菌短程硝化的影响,。2。2,30℃,。,20℃,30℃43.1%,ArrheniusNH+4-N62.1kJ/mol,WILLKE[10]NH+4-N63.6kJ/mol。2　Fig.2　EffectoftemperatureonNH+4-Noxidationrateandshort-cutnitrificationefficiency　　20～30℃,25℃,,。HELLIGA[3]135-142BALMELLE[11]1243-1258,30～35℃NO-2。,。2·14·　　30　11　200811。2,15～35℃,85%,30℃,94.7%。NO-2-N,。2.2　pH对固定化氨氧化细菌短程硝化的影响pH,:pH;(3),pHFA,。cFA=1714×cNH+4-N×10n[exp(6334273+T)+10n](3):cFAFA,mg/L;cNH+4-NNH+4-N,mg/L;npH;T,℃。　　3pH。3,pH,,pH8.5。pH7.0～9.0,,。,NH3NH+4,pH,NH3,pHNH3。3　pHFig.3　EffectofpHonNH+4-Noxidationrateandshort-cutnitrificationefficiency　　3pH。3,pH7.0,90%,pH6.0,85%。,pH(7.0～9.0)。2.3　碱度对固定化氨氧化细菌短程硝化的影响pH(1molNH+4-N2molHCO-3,1gNH+4-N7.14g),。4HCO-3。4,HCO-3,HCO-3,HCO-3,。SA-KAIRI[12]。4HCO-3,HCO-3≥4mmol/L,94%。4　HCO-3Fig.4　EffectofHCO-3dosageonNH+4-Noxidationrateandshort-cutnitrificationefficiency　　5NH+4-N、NO-2-N,,R20.9949、0.9636。NH+4-N,1gNH+4-N6.75g,7.14g,5.5%。NO-2-N,1gNO-2-N7.71g。1.00g0.15gNH+4-N0.13gNO-2-N,1.00g,NH+4-N87.5%NO-2-N,。5,。5　NH+4-N、NO-2-NFig.5　CorrelationsofNH+4-NconsumptionandNO-2-Nproductionwithalkalinityconsumption·15·　2.4　溶解氧对固定化氨氧化细菌短程硝化的影响,NH+4-NNO-2-NNO-3-N,。,。6。6,4.03mg/L,;4.03mg/L,,,。,4.03mg/L。,,;,,,,,,。6,1.56～5.60mg/L(90%),。BALMELLE[11]1243-1258,NO-2-N,。6　Fig.6　EffectofDOonNH+4-Noxidationrateandshort-cutnitrificationefficiency3　　(1),、pH30℃8.5,pH(7.0～9.0)NO-2-N;,。(2),。NH+4-N、NO-2-N,R20.9949、0.9636,/NH+4-N()6.75,87.5%。(3),,,4.03mg/L。[1]　,,.[M].:,2004.[2]　.[M].:,2006.[3]　HELLIGAC,SCHELLENAAJC,MULDERJW,etal.TheSHARONprocessaninnovativemethodfornitrogenremovalfromammoniumrichwastewater[J].Wat.Sci.Tech.,1998,37(9).[4]　,,,.[J].,2006,28(4):254-256.[5]　,,.[J].,2003,25(6):332-335.[6]　,,.[J].,2004,24(5):618-622.[7]　TANAKAK.Applicationofnitrificationbycellsimmobilizedinpolyethyleneglycol[J].Prog.Biotech.,1996,11:622-632.[8]　,.SBR[J].,2001,24(8):674-678.[9]　《》.[M].4.:,2002.[10]　WILLKETH,VORLOPKD.NitrificationinPVALbeads:influenceofpHandtemperatureonnitriteoxidation[J].Prog.Biotech.,1996,11:718-724.[11]　BALMELLEB.Studyoffactorscontrollingnitritebuild-upinbio-logicalprocessesforwaternitrification[J].Wat.Sci.Tech.,1992,26(5/6).[12]　SAKAIRIMAC,YASUDAK,MATSUMURAM.Nitrogenremovalinseawaterusingnitrifyinganddenitrifyingbacteriaimmobilizedinporouscellulosecarrier[J].Wat.Sci.Tech.,1996,34(7/8):267-274.责任编辑:贺锋萍　(:2008-01-31)(上接第12页)[3]　,,,.—[J].,2002,18(4):37-39.[4]　,.[J].,2003,19(5):59-60.[5]　,.[J].,2001,23(3):107-110.[6]　,.[J].,2003,25(6):345-347.[7]　BRYEKR,NORMANJM,BUNDYLG.Nitrogenandcarbonleachinginagroecosystemsandtheirroleindenitrificationpo-tential[J].J.Environ.Qual.,2001,30:58-70.[8]　YBERGPMFN.Infiltrationofwastewaterinanewlystartedpilotsand-filtersystem:Ⅱdevelopmentanddistributionofthebacterialpopulation[J].J.Environ.Qual.,1989,18:457-462.[9]　.[J].,1997,17(5):556-560.[10]　,,.[J].,2000,20(1):168-172.[11]　,,.()[M].4.:,1999.责任编辑:黄　苇　(:2008-07-23)·16·　　30　11　200811