固定化氨氧化细菌短程硝化稳定性研究李正魁

1,2,1,1,2,1,1(1.,,　210093;2.,　210093):,,、HRT、(FA).,100、150200mgL,10mgL;25.8、51.193.3mgL,3、6、12h,,,,(HRT);(FA)9mgL,;,,,,.:;;;:X703.1　:A　:0250-3301(2008)10-2835-06:2007-10-10;:2008-01-24:(973)(2008CB418003);(2006BAJ08B01-02);(JS2004ZB02);(WTWER0707):(1959～),,,,,E-mail:[e-mail]-cutNitrificationUsingImmobilizedAmmonia-oxidizingBacteriaLIZheng-kui1,2,LAIDing-dong1,YANGZhu-you1,2,ZHANGXiao-jiao1,LIFang-jie1(1.StateKeyLaboratoryofPollutantControlandResourcesReuse,SchoolofEnvironment,NanjingUniversity,Nanjing210093,China;2.EngineeringCenterofWaterTreatmentandEnvironmentRemediation,MinistryofEducation,NanjingUniversity,Nanjing210093,China)Abstract:Theammonia-oxidizingbacteriawereimmobilizedbycopolymerwithcellproliferationtechnology.TheeffectsofNH+4-Nload,HRT,freeammonia(FA)andorganicmatteronshort-cutnitrificationprocesswerestudied.TheresultsshowedthatwheninfluentNH+4-Nloadwere100mgL,150mgLand200mgLrespectively,effluentNH+4-Nconcentrationwaslessthan10mgL.Whenthesystemrunfor3h,6hand12h,correspondingtoinfluentNH+4-Nconcentrationof25.8mgL,51.1mgLand93.3mgLrespectively,NH+4-Nconcentrationwaslowineffluentwithhighshort-cutnitrificationefficiency.TheHRTcouldbeadjustedtooptimizethesystemoperationwithvariationofinfluentNH+4-Nconcentration.Theresultsalsoindicatedthattheammonia-oxidizingbacteriawererestrainedwhilefreeammoniaconcentrationwasover9mgL.Theactivityoftheammonia-oxidizingbacteriacouldbeenhancedunderexistenceoflow-molecular-weightorganiccompounds,buttheshort-cutnitrificationefficiencywaslittleaffected.Inaddition,theshort-cutnitrificationanddenitrificationcouldberealizedwithexistenceoforganiccompoundsduringtheexperiment.Keywords:immobilized;ammonia-oxidizingbacteria;short-cutnitrification;stability　　-2,,NO-2-N.,、DO、pH,,NO-2-N[1～14]..SHARON,(solidresidencetime,SRT)(hydraulicretentiontime,HRT),2:,,;,,[15].,,,.,,、HRT、(FA),NO-2-NNO-2-N,-.1　1.1　2910200810　　　　　　ENVIRONMENTALSCIENCEVol.29,No.10Oct.,2008DOI:10.13227/j.hjkx.2008.10.023β-(2-Hydrosyethylmethylacrylate,HEMA,Fluka,)、(2-Hydroxyethylacrylate,HEA,Fluka,)..1.2　PHS-3CpH()、T52N()、THZ-82A()、60Co-γ()、5000ATOC().1.3　.:(NH4)2SO42.0g、K2HPO40.5g、MgSO40.5g、FeSO4.7H2O0.4g、NaCl0.5g、Na2CO31.0g,1000mL,pH7.2.1.4　β-(HEMA)、(HEA),,,-78℃(-),1×104Gy60Co-γ,5mm×5mm×5mm,1[16,17].1.5　,28℃,3.5×108mL(MPN),,28℃24h3,,500mL,,.1.6　1.1.7L(8cm,50cm)(30℃±1℃),,,,10%,,NH+4-N、NO-2-NNO-3-N.1.7　、1.;2.;3.;4.;5.;6.;7.;8.;9.1　Fig.1　Schematicdiagramofexperimentalreactor　,,,P、Fe、Mg、Ca、Co,NaHCO3,,pH7.5.,,N-(1-)-[18].TOCTOC(TOC-5000A),pHPHS-3CpH.:=(NH+4-N-NH+4-N)t(1)=NO-2-NNO-2-N+NO-3-N×100%(2)TN=NH+4-N+NO-2-N+NO-3-N(3)=TN-TNTN×100%(4),t.2　2.1　2,3.3、.,3(100、150、200mgL),,10mgL,,,2d,.3,,,,2836　　　　　　2995%,.2,3,,,3,3,90%,,1,,,、.2　Fig.2　Variationofinorganicnitrogenconcentrationofinfluentandeffluentatdifferentammonianitrogenload　3　Fig.3　Ammonianitrogenremovalefficiencyandshort-cutnitrificationefficiencyatdifferentammonianitrogenload　2.2　HRT3,,.43.,25.8、51.193.3mgL,、4　HRTFig.4　VariationofinorganicnitrogenwithHRTatdifferentammonianitrogenconcentrationofinfluent,3、612h.25.8、51.193.3mgL,7.43、7.577.77mg(L·h),.,HRTSRT,HRT,SRT,.,,　　　　　　283710:,HRT.,,SHARONHRTSRT.2.3　(FA)pHNH3(FA)NH+4-N.NH+4-NFA(5)[5]:NH3+H2ONH+4+OH-(5)　　FApHNH+4-N,Anthonisen[5]:FA=1714×NH+4-N×10pHKbKw+10pH(6)KbKw=exp6334(273+T)(7),KbNH+4-N;Kw.(FA):(30±1)℃,86.7～716.7mgL,pH,pH7.5.(5)(6)(FA)2.71～15.8mgL.5,(FA),FA=9mgL,.,NH3NH+4,NH3,[15].5,(FA)9mgL.5,(FA),2.71～15.8mgL,85%～95%,.,,(FA),.2.4　:100mgL,12h,,5　Fig.5　Ammonianitrogenoxidationrateandshort-cutnitrificationefficiencyatdifferentFAconcentration　[23].6,(TOC).6,TOC25、30、60100mgL8.51、9.18、9.039.04mg(L·h),8.37mg(L·h)1.70%、9.45%、7.23%7.48%,.6　Fig.6　Ammonianitrogenoxidationrateandshort-cutnitrificationefficiencyatdifferentorganicmatterconcentration　7.,.:、.,pH.pH8.5,[19～21].pH8.0,.2838　　　　　　29,(1.8%)[22,23].7,,65%,1.8%.,,.,;(),.Abeliovich[24,25]:(Nitrosomonaseutropha),;,;,;,Nitrosomonaseutropha、.,,.7　Fig.7　RelationbetweenTNlossandorganicmatterdecrement3　　　(1)、.,HRT,.(FA)9mgL,,(FA),(FA),.(2),.:[1]　,,,.[J].,2006,27(12):2472-2476.[2]　,,.[J].,2005,26(1):63-67.[3]　,,.[J].,1999,25(7):37-39.[4]　HelligaC,SchellenAAJC,MulderJW,etal.TheSHARONProcessaninnovativemethodfornitrogenremovalfromammoniumrichwastewater[J].WatSciTech,1998,37(9):135-142.[5]　.[M].:,2006.39-64.[6]　Aktas,eenF.Nitrificationinhibitioninlandfillleachatetreatmentandimpactofactivatedcarbonaddition[J].BiotechnolLett,2001,23(19):1607-1611.[7]　Mosquera-CorralA,GonzálezF,CamposJL,etal.PartialnitrificationinaSHARONreactorinthepresenceofsaltsandorganiccarboncompounds[J].ProcessBiochemistry,2005,40(9):3109-3118.[8]　RheeSK,Leell,LeeST.Nitriteaccumulationinasequencingbatchreactorduringaerobicphaseofbiologicalnitrogenremoval[J].BiotechnolLett,1997,19(2):195-198.[9]　JeisonD,RuizG,AcevedoF,etal.Simulationoftheeffectofintrinsicreactionkineticsandparticlesizeonthebehaviourofimmobilisedenzymesunderinternaldiffusionalrestrictionsandsteadystateoperation[J].ProcessBiochemistry,2003,39(3):393-399.[10]　AllemanJE.Elevatednitriteoccurrenceinbiologicalwa