搜索
·371·200111186JEnvironHealth,November2001,Vol.18,No.6【】:1001_5914(2001)06_0371_03谭佑铭,罗启芳。(UASB),60,。(PVA),,,。(),。,。pH5～35℃6.0～9.0,,6h90%。(C/N)3.5,(HRT)4h,95.9%。,,pH。;;;:R123:AStudyonRemovalofNitrateFromDrinkingWaterbyImmobilizedDenitrifyingBacteria.TANYou_ming,LUOQi_fang.Inst.ofEnviron.Medicine,TongjiMedicalSchool,HuazhongUniv.ofSci.Tech,Wuhan430030AbstractObjectiveTostudythecharacterandaffactingfactorsofdenitrificationofimmobilizeddenitrifyingbacteria.MethodsDenitrifyingbacteriawereculturedinapilotscaleupstreamanaerobicsludgeblanket(UASB)reactorwithbrewerywastedegradingsludgeasseed.Thecountofcultureddenifrifyingbacteriaincreased60times,anddenitrificationefficiencywereimprovedalso.Immobilizedbacteriatechnologywasemployedwithpolyvinylalco-holasentrapmentagentwhichwasmixedwithactivatedcarbonandsodiumalginateandmadeasimmobilizedsmallballbodiesfortreatingwatersampleswithdifferentconcentrationsofnitrateundervarioustreatmentconditions.Activatedcarbonandsodiumalginatewereaddedasadsorbentandcross_linkingreagentrespectively,whichcouldimprovethemechanicalstrengthofimmobilizedbacteria.ResultsNitratecouldberemovedeffectivelybyimmobi-lizedbacteriaatthepresentofmethanolasaddedorganiccarbon.Thedenitrificationefficiencyandvelocityhadsomethingtodowithaddedorganiccarbon.Toacertainextent,thedenitrificationspedupastheorganiccarbonincreased.TheimmobilizedcellsperformedhighactivitywhenwatertemperatureandpHvaluechangedfrom5℃～35℃and6.0～9.0respectively,andthedenitrificationeffciencycouldreach90%within6h.WhenC/N=3.5,hy-draulicretentiontime(HRT)=4h,thedenitrificationefficiencyis95.9%.ConclusionTheimmobilizeddenitrifyingbacteriacouldbeemployedtoremovenitratefromdrinkingwaterwithhighperformanceatthepresentofadequateorganiccarbon.ItalsohadastrongresistancetowatertemperatureandpHvaluechanging.KeywordsNitrate;Immobillizedbacteria;Denitrification;Polyvinylalcohol(PVA),,。,。,。:①,,;②;③。(Polyvinylalcohol,PVA),,,[1]。。11.1仪器与试剂1.1.1仪器LD5_2A();722();KS()。1.1.2试剂(PVA),1750±50();,(),160;,()。。1.2反硝化菌的培养,2d,,(Upflowanaerobicsludgeblanket,UASB),1/3。。。,COD∶N∶P=200∶5∶1。Mo2+、Mg2+、Ca2+、Cu2+5×:(39870664):(430030):(1969_),,,,。DOI:10.16241/j.cnki.1001-5914.2001.06.031·372·200111186JEnvironHealth,November2001,Vol.18,No.60(h)2(h)4(h)6(h)8(h)00.51.01.52.02.53.03.50.00.00.00.00.00.00.00.010.345.546.258.160.470.171.576.323.255.268.880.078.491.289.495.925.358.290.193.191.492.693.896.725.961.698.796.896.696.696.697.5(%)CN1CN0(h)2(h)4(h)6(h)8(h)6.07.08.09.00.00.00.00.066.3267.0259.1954.7393.8794.1691.7086.6497.4397.9396.8593.28(%)pH2pH86.4485.0581.5873.6610-4mol/L。,(DO)2mg/L,pH6.8～7.0。DO、pH、NO3-_N、NO2-_N、NH4+_N、COD[2],MPN[3]。,。,NO3-_N5mg/L,90%,NO3-_N,7NO3-_N100mg/L,99%,,2mm,。1.3固定化反硝化菌小球的制备[4]。PVA8%、10%、12%。PVA,20ml,ф=3mm,3～5mm。1.4反硝化动力学研究1.4.1无碳源时的反硝化作用66g(30g)500ml,NO3-_N50mg/L(KH2PO4、K2HPO4、Ca2+、Mg2+、Mo2+)250ml,20℃,0、2、4、6、8h,NO3-_N。1.4.2以甲醇为碳源的反硝化作用250ml,,CN()0.5、1.0、1.5、2.0、2.5、3.03.5。,1.4.1。1.5水样pH值对反硝化的影响250ml,CN=3.5。,pH6.0、7.0、8.0、9.0。,1.4.1。1.6温度对反硝化的影响CN=3.5250ml,5、15、25、35℃,1.4.1。22.1反硝化细菌计数、4.0×104/g2.5×106/g,60。,。2.2污泥的反硝化性能NO3-_N99.2mg/L,UASBNO3-_N、NO2-_NNH4+_N0.68mg/L、38.6μg/L4.0mg/L。,N2。2.3固定化小球物理性状评价,,3Hz。PVA8%,,;PVA12%,,,,。10%PVA,,200h。,PVA10%。2.4小球驯化NO3-_N5mg/L,,,。,PVA。,,。2NO3-_N50mg/L,95%,,。。2.5固定化细菌的反硝化动力学,CN,1。1,。,,。4h0.016mgNO3-_N/(h·g),4h,8h25.9%。,,CN,,4h2h。,,。CN3.5,(HRT)4h,1.36kg/(m-3·d)。2.6不同因素对反硝化作用的影响2.6.1pH值对反硝化作用的影响pH,2。2,pH。pH6～7,pH;pH8～9,pH,。·373·200111186JEnvironHealth,November2001,Vol.18,No.6[1][2][3][4]0(h)2(h)4(h)6(h)8(h)51525350.00.00.00.065.4365.3379.6084.4692.1996.4499.3899.3895.2597.4499.5299.83(%)(℃)383.4695.1695.1097.14:1001_5914(2001)06_0373_01:(625000)李一成,张世超,朱正强,魏洪贵,黄莉,付洪仁:X522:C【】,。,、、、()4015t,2498t,4t(),6049t,604t,18.36t,108t,10%。、、、、、、,,。1998,62t,1095t,,,,。,、、、、,1400。,,、、,。26,16,61.53%,100%;240,900,801,20,,。5,,。,1,0.6mg/L,1。、、、、、、、DDT、12,316,1,,0.07mg/L,、DDT,。15,,9,。,α(0.130±0.003),,,。,GB3838_1988,Ⅲ,,。(:2001_06_21)(:)2.6.2温度对反硝化作用的影响,3。3,,,。25～35℃,,2h80%。5℃,。3,。,。。CN=3.5,HRT4h,95.9%。pH。4,.[J].,1997,18(2):18_20..[M].,:,1989,246_356.,.[M].:1985.229_238.,.[J].,1994,15(3):11_14.(:2001_06_20)(:)