固定化反硝化细菌脱氮的研究田晋红

第26卷第3期　　　西　南　农　业　大　学　学　报(自然科学版)Vol.26,No.32004年6月JournalofSouthwestAgriculturalUniversity(NaturalScience)Jun.2004:1000-2642(2004)03-0318-04:2003-12-17:(000501):(1958-),,,,,。田晋红,熊　平,董莉仙,张传斌(,　402460):以反硝化细菌为例,研究了PVA包埋和未包埋的反硝化细菌脱氮特性。采用PVA纱布块包埋反硝化细菌,对其在不同氮源、不同温度和不同铵盐浓度下的脱氮特性进行研究,并与未包埋反硝化细菌进行比较。结果表明,固定化反硝化细菌对废水脱氮的最适温度未变,为30℃,固定化反硝化细菌对NO2--N150mg/L脱氮速率在10℃和30℃分别是19.23和60.00mg/(g·h),而未固定化的只有6.89和25.6mg/(g·h)。固定化硝化细菌对NO3--N150mg/L脱氮速率在10和30℃分别是10.87和42.86mg/(g·h),而未固定化的只有3.05和15.86mg/(g·h)。固定化和未固定反硝化细菌在NH4+浓度为200mg/L时的脱氮速率分别是25.90和13.90mg/(g·h)(NO2--N150mg/L,30℃),29.10和14.90mg/(g·h)(NO3--N150mg/L,30℃)。说明固定化反硝化细菌对高浓度的铵离子和低温耐受性增加。但固定化反硝化细菌对NO2-的脱氮速率小于对NO3-的脱氮速率。　　:反硝化细菌;固定;脱氮:Q93-33　　　　　　:ADENITRIFICATIONCHARACTERISTICSOFIMMOBILIZEDDENITRIFYINGBACTERIATANGJin-hong,XIONGPing,DONGLi-xian,ZHANGChuan-bin(SouthwestAgriculturalUniversity(RongchangCampus),Rongchang,Chongqing402460,China)Abstract:Thedenitrificationcharacteristicsofdenitrifyingbacteriaimmobilizedbypolyvinylalcohol(PVA)werestudiedwithdifferentNsourcesandatdifferenttemperaturesandNH4+concentrationsandcomparedwiththoseoffree(non-immobilized)bacteria.Theoptimumtemperaturefordenitrifyingliquidwastewas30℃forbothimmobilizedandfreedenitrifyingbacteria.ThedenitrificationrateofNO2—NwaslowerthanthatofNO3—Nintheimmobilizeddenitrifyingprocess.ImmobilizeddenitrifyingbacteriaweremoretoleranttolowtemperatureandhighNH4+thanfreedenitrifyingbacteria.Keywords:denitrifyingbacteria;immobilization;denitrification　　。,2。,[1]。[2～4],。,PVA。1　1.1　(PVA),,。。1.2　25min,4500r/min,,。1g,,。1.3　(PVA)1080℃,,,30℃。PVA,,,,,11.5℃24h。,,10L,[2]。1.4　1.4.1　NO2--NNO3--N30℃,pH7.5,,。1.4.2　NO2--NNO3--N,,。1.4.3　NO2--NNO3--N,,。NO3--NNO2--N,NO2--N-[5～6]。2　2.1　10L30g,,1g2400,10L72000。NO3-NO2-N2。/[μg(g·h)-1]　=NO2--NNO3--N/μg/g·/h2.2　NO3--NNO2--N30℃,pH7.5,1,NO3--NNO2-N,,,NO3--N32mg/(g·h),NO2--N29mg/(g·h),6.5mg/(g·h)13mg/(g·h)。2.3　50mg/LNO3--NNO2--N2。pH=7.5,10℃NO3--NNO2-N3.5mg/(g·h)5.5mg/(g·h),11.111.9mg/(g·h)。40℃11.812.7,33.425.7mg/(g·h)。2.4　150mg/LNO2--NNO3--N,,3。,30℃,,。2.5　[NH4+-N]=100mg/L,NO3-,NO2-,4。30℃,pH7.5,NH4+-NNO3--NNO2--N,NO3--NNO2--N,200mg/LNO3--NNO2--N1417mg/(g·h)。1　[NO2--N][NO3--N]./[mg·(g·h)-1]Table1　ComparedwithdenitrificationrateofimmobilizeddenitrifyingbacteriaandfreebacteriaindifferentNO3--NorNO2--Nconcentration　bacteria　nitrogen/Lvolume/NO2-N/NO3-N　NO3--NorNO2--Nconcentration/(mg·L-1)50100150200/htimedenitrifica-tionrate/htimedenitrifica-tionrate/htimedenitrifica-tionrate/htimedenitrifica-tionrateimmobilizeddenitrifyingbacteriaNO2--N101.2013.891.5522.002.2622.702.4327.80freebacteriaNO2--N50.6512.801.5711.031.8713.502.0815.90immobilizeddenitrifyingbacteriaNO3--N101.0715.201.4323.801.6513.502.0033.30freebacteriaNO3--N51.954.162.935.763.237.803.479.52319第26卷第3期　　　　　　　　　田晋红等　固定化反硝化细菌脱氮的研究2　NO3-NO2-./[mg·(g·h)-1]Table2　Comparedwithdenitrificationrateofimmobilizeddenitrifyingbacteriaandfreebacteriain50mg/LNO3--NorNO2--Nunderdifferenttemperature　bacteria　nitrogen/Lvolume/NO2-N/NO3-N50(mg·L-1)　NO3--NorNO2--Nconcentration50(mg·L-1)10℃20℃30℃40℃/htimedenitrifica-tionrate/htimedenitrifica-tionrate/htimedenitrifica-tionrate/htimedenitrifica-tionrateimmobilizeddenitrifyingbacteriaNO2--N101.4011.931.2013.920.4338.830.5033.40freebacteriaNO2--N51.505.530.6512.700.5315.600.6512.70immobilizeddenitrifyingbacteriaNO3--N101.5011.101.0715.900.6027.800.6725.70freebacteriaNO3--N52.383.491.954.201.425.900.7211.863　150(mg·L-1)NO2--NNO3--N./[mg·(g·h)-1]Table3　Comparedwithdenitrificationrateofimmobilizeddenitrifyingbacteriaandfreebacteriain150mg/LNO3--NorNO2--Nunderdiffer-enttemperature　bacteria　nitrogen/Lvolume/NO2-N/NO3-N150(mg·L-1)　NO3--NorNO2--Nconcentration150(mg·L-1)10℃20℃30℃40℃/htimedenitrifica-tionrate/htimedenitrifica-tionrate/htimedenitrifica-tionrate/htimedenitrifica-tionrateimmobilizeddenitrifyingbacteriaNO2--N102.6019.231.8726.790.8360.000.9552.63freebacteriaNO2--N53.636.892.2711.030.9725.861.0523.80immobilizeddenitrifyingbacteriaNO3--N104.6010.871.6530.301.1742.861.3337.50freebacteriaNO3--N58.203.053.237.731.6015.632.759.092.6　[NH4+-N]=200mg/L,NO3--NNO2--N,5。NH4+-NNO3--NNO2--N,8.58.9mg/(g·h),NO3--NNO2--N,200mg/LNO3--NHNO2--N150mg/L。3　3.1　1,,。,,NO2--NNO3--N,NO2--NNO3--N,。,50～100mg/L,。3.2　23,30℃,,,[2～3,7]。,,[2]。,,,,[7～8],。3.3　45,NH4+,NO3-NO2-,,NH4+。NH4+,,NH4+[9]。NH4+200mg/LNO2--N200mg/L,320西　南　农　业　大　学　学　报　(自然科学版)　　　　　　　　2004年6月,NO3--N3327mg/(g·h)。30℃,,。NH4+,,,。,NO2-NO3-,PVA,NO3-NO2-。4　NH4+100(mg·L-1)./[mg·(g·h)-1]Table4　ComparedwithdenitrificationrateofimmobilizeddenitrifyingbacteriaandfreebacteriaindifferentconcentrationNO3--NorNO2--Nwith100mg/LNH4+-N　bacteria　nitrogen/Lvolume/NO2-N/NO3-N　NO3--NorNO2--Nconcentration(mg·L-1)50100150200/htimedenitrifica-tionrate/htimedenitrifica-tionrate/htimedenitrifica-tionrate/htimedenitrifica-tionrateimmobilizeddenitrifyingbacteriaNO2--N100.7522.201.5022.201.9325.903.2320.70freebacteriaNO2--N50.5315.601.1015.201.8013.902.4213.8immobilizeddenitrifyingbacteriaNO3--N100.5828.601.1828.271.7229.102.2829.20freebacteriaNO3--N50.6313.201.3512.301.6814.901.8817.705　NH4+200(mg·L-1)./[