搜索
,,.[J].,2005,25(2):172-175CHENYuancai,LANHuixia,CHENZhonghao.Degradationofchlorophenolbyco2immobilizedanaerobicgranularsludgeandaerobicmicrobesindifferentoxygenconcentration[J].ActaScientiaeCircumstantiae,2005,25(2):172-1751,2,1,11.,510641;21,510650:4,2887molL-1(PCP).,PCP,(MCP)(DCP),,;,,,;,TOC..:;;:025322468(2005)0220172204:X703:ADegradationofchlorophenolbyco2immobilizedanaerobicgranularsludgeandaero2bicmicrobesindifferentoxygenconcentrationCHENYuancai1,2,LANHuixia1,CHENZhonghao11.StateKeyLaboratoryofPulp&PaperEngineering,SouthChinaUniversityofTechnology,Guangzhou510641;2.LaboratoryofCelluloseandLignocellulosicsChemistry,AcademyofSciences,Guangzhou510650Abstract:Thedegradationofpentachlorophenol(PCP)wasstudiedinbatchexperimentsusingco2immobilizedanaerobicgranularsludgeandfouraerobicbacterialspeciesatlowandhighinitialPCPconcentration(28and87molL-1)underoxygen2limitedanaerobicandaerobiccondition.TheresultssuggestedthatalthoughPCP(pentachlorophenol)couldbedegradedatlowinitialPCPconcentrationwith0IHOP,whileathighinitialPCPconcentration,theresidualPCPconcentrationwerecorrelatedhighlywiththeIHOP,thehigherPCPreductionandlowerintermediateswereachievedbythesystemreceiving30and50IHOP.Duringtheanaero2biccondition,theintermediatesMCPandDCPmightexertsubstratetoxicityonoxygenaseexpressingcultures,whichwouldinhibitoxidationofitsowndegradation.Howeverinoxygen2limitedcondition,thehighdechlorinationefficiencymightbeduetotheco2metabolismMCPandDCPbyaerobicdegradationbacteria;aerobicorfacultativebacteriahadbeenfoundtooxidizelesschlorinatedordechlorinatedcompoundsproducedinsynergisticassociationwithmethanogensandstrictlyanaer2obesduringfermentationduetothecombinationofreductiveandoxidativedegradationmechanisms.Theoxygen2limitedsystemalsoexhibitedthelowerresidualTOCconcentration,whereastheanaerobicsystemexhibitedhigherresidualTOCconcentration.Theseresultssuggestedimmobilizationofanaerobicandaerobicmicrobialcommunitiesunderoxygen2limitedconditionsmightbeemployedasanoptimalstrategytomineralizemorehighlychlorinatedaromaticcompoundwithlowfinalTOCconcentrations.Keywords:oxygen2limitedcondition;PCP;immobilizedcell:2004203201;:2004209201:(31430);(200335);(K-147):(1967),,,,atep@scut.edu.cn,,,.,,,25220052ACTASCIENTIAECIRCUMSTANTIAEVol.25,No.2Feb.,20052,[13].PVA,,COU2PLED,UASB,2,4,62,[4].,,.1111TOCModel100BenchtopTOCAnalyzer;AUTOSYSTEMXL,PER2KIN2ELMER;:50mL10mL,pH=210min,,10mL3,,Na2SO4,2mL;:DB25(15m0132mm0125mm),:801min,10min-1280,ECD,350,:,:280,1L.1121.2.1[5],10mgL-1,3,30mgL-1,60mgL-1,100mgL-1.1.2.2(42CP(),2,42DCP(2,42),2,4,62TCP(2,4,62),PCP()),NH4NO3,011gL-1;Ca(NO3)2,0102gL-1;FeSO47H2O,0101gL-1;(NH4)2SO4,011gL-1;MgSO47H2O,011gL-1;K2HPO4,0105gL-1;KH2PO4,0103gL-1,2%.,,pH7,.1.2.3,VitekAmst,:VitekAmst232,GNI,GPI,BAC,,24h.,7,4En2terobactercloacae(entclo)Corynebacte2riumxerosis(corxer)PseudomonasKlebsiellapneumoniae(klepne).1.2.4150mL8%PVA2%,150mL(110gL-1),,10min,,3mm,4%CaCl25h,CaCl2,,-15,,3,,pH7,.1.2.5250mL,75mL,(2gL-1)(015gL-1)(015gL-1),150mL,NaOHpH710,5min,.,,,(IHOP),,34.50.0145m,TOC,PCP.2.:Fe(NH4)26H2O(2mgL-1),NiCl26H2O(4mgL-1),CoCl26H2O(4mgL-1),(NH4)2HPO4(60mgL-1);Na2S9H2O(60mgL-1)Na2HCO3(4000mgL-1)12(2887molL-1)3712:4(10,30,50,100IHOP),(0IHOP)(0IHOP,)6PCP.2.,,0145m,PCP.12,PCP(1(a)),34dPCP,.PCP(1(b)),PCP,49d30IHOPPCP,(0IHOP)PCP.3PCP87molL-11PCPIHOPFig.1PCPversustimeatinitial28molL-1(a)and87molL-1(b)withvariousIHOP2PCPIHOP(PCP28molL-1(a)87molL-1(b))Fig.2PCPintermediatesmetabolitesdistributionatinitial28molL-1(a)and87molL-1(b)withvariousIHOPTOC.PCP87molL-1,50IHOP100IHOPTOC2400mgL-11520mgL-11532mgL-1,(0IHOP)TOC1799mgL-1.312(2887molL-1)PCP,.PCP(1(a)),PCP,34dPCP,PCP(1(b)),PCP3IHOPTOC(PCP:87molL-1,1)Fig.3TOCversustimeinvariousIHOPatinitial87molL-1PCP,thesymbolsweresameasFig1.47125PCP(2(a)),PCP,34d,PCP,.PCPMCPDCP,(IHOP),(0IHOP),,50IHOPMCPDCP,.PCP(2(b)),(0IHOP),PCP,MCPDCP,TCP,;(30,50IHOP),PCP,PCP,MCPDCP,..PCP.12,,,,;,,,.350IHOP100IHOP,TOC,(0IHOP)TOC.,MCPDCP,MCPDCP,,.TOCPCP,PCP.4PVA,,2887molL-1(PCP).PCP(28molL-1),(87molL-1),,;,,,;,TOC.:[1]HGardin,A.Pauss.K2carrageenanPgelatingelbeadsfortheco2im2mobilizationofaerobicandanaerobicmicrobialcommunitiesdegrading2,4,62trichlorophenolunderair2limitedcondition[J].ApplMicrobiolBiotechnol,2001,56:517523[2]TartakovskyB,MiquezCB,PettiL,etal.Tetrachloroethylenedechlorinationusingaconsortiumofco2immobilizedmethanogenicandmethanotrophicbacteria[J].EnzMicro2biolTechnol,1998,22:255260[3]ZitomerDH,ShroutJD.Feasibilityandbenefitsofmethanogenesisunderoxygen2limitedconditions[J].WasteManagement,1998,18:107116[4]ChenYuancai,ZhanHuaiyu,ChenZhonghao.CoupledanaerobicPaerobicbiodegradationofHalogenatedphenoliccompounds[J].Jour2nalofEnvironmentalScience,2003,15(4):469474[5]YuancaiChen,ZhonghaoChen,HuaiyuZhan.Studyonthetreat2mentofthesulfitepulpCEHbleachingeffluentswiththecoagulation2anaerobicacidificationpackagereactor[J].WaterResearch,2003,37:210621125712: