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1998,18(4):356～359 ChinaEnvironmentalScience(Methanosarcina)———＊ ＊＊ (,100037) UASB。、COD、(HRT)、(、)、(SEM)(TEM),。,50d,5.3L/L·d,8.1L/L·d,COD3000～5500mg/L800～3000mg/L,HRT10.4h,、264.1、519.8104.6mg/L,60.6%,,0.5～3.0mm,119.6m/h,(Methanosae-ta),(Methanosarcina)。 (UASB) (Methanosaeta)StudyonimmobilizedMethanosarcina—formationofanaerobicgranularsludge.YangXiushan,TianShen,ZhengYing,ZhangWei,LiuMing,WangHongjie(DepartmentofBiology,CapitalNormalUniversity,Beijing100037).ChinaEn-vironmentalScience.1998,18(4):356～359Abstract—TheUASBreactoroflabscalewasusedtotreatsoybeancakewastewaterinordertoincubateanaerobicgranu-larsludge.Gasproductionrate,influentandeffluentCOD,HRT,VFAconcentration(acetate,propionateandbutyrate)andmathanecontentweredetermented,andthepredominantmethanogenswereobservedbySEMandTEMtodemon-stratetheprocessandcharacteristicsofanaerobicgranularsludge.Theresultsindicatedthatanaerobicgranularsludgewasformedduringtheperformanceof50days;averagegasproductionratewas5.3L/L·d;thehighestgasproductionratereachedupto8.1L/L.d,influentandeffluentCODwas3000～5500mg/Land800～3000mg/Lrespectively;averageHRTwas10.4h;averageconcentrationofeffluentacetate,propionateandbutyratewere264.1mg/L,519.8mg/L,and104.6mg/Lrespectively;averagemethanecontentwas60.6%,granularsludgecontainingmainlyMethanosaetaandlessMethanosarcinawasdarkwithdiameterof0.5～3.0mmandprecipitationrateof119.6m/h.Keywords:UpflowAnaerobicSludgeBlanket(UASB) anaerobicgranularsludge Methanosaeta (Methanosarcina)(Methanosaeta)〔1〕。UASB,。,,。70(UpflowAnaerobicSludgeBlanket,UASB)〔2〕,UASB。UASB〔3〕。,UASB,。,:1997-09-09＊＊＊95。UASB,,UASB。。UASB,,。1 1.1 UASB600mm,,1.2L。1.2 。22.5gTSS/L,14.5gVSS/L。1.3 ,COD3000～5500mg/L,8～10℃。pH5.0。,35℃。1.4 COD、〔4〕COD、。2 2.1 UASB63d,1。1 Fig.1 Gasproductionrateduringtheperformance 1,10d2.5L/L·d,,37d,7.8L/L·d,8.1L/L·d,10d5.3L/L·d。38dCOD,2。2 CODFig.2 CODConcentrationsofinfluentandeffluent1.COD 2.COD 2COD,COD3000～5500mg/L,COD800～3000mg/L。COD51%～72%。HRT3。3 HRTFig.3 Hydraulicretentiontimeduringtheoperation UASB9d,HRT,160h。10d,HRT,HRT6.8h。10dHRT10.4h。39d、43d、50d、57d62d64.6%、53.1%、65.7%、68.9%50.1%,60.6%。。38d、15,3574 :(Methanosarcina)— 4。714.6mg/L,264.1mg/L;89.2mg/L,519.8mg/L;75.1mg/L,104.6mg/L。1。4 Fig.4 Acetateconcentrationofinfluentandeffluent1. 2. 50d。、COD、COD、HRT、,COD、HRT。,COD,UASB,,。2.2 ,(5A),,(5B5C)。(5D)。A B C D5 Fig.5 SEMandTEMmicriographofanaerobicgranularsludgeA. B.C. D. 0.5～3.0mm,。、、、,,。,,。1996〔3〕。〔3〕,18～100m/h,18～50m/h。3:1358 18,20m/h;2,20～50m/h;3,50m/h。23。79～180m/h,119.6m/h,。3 3.1 UASB,50d,0.5～3.0mm,,119.6m/h。3.2 5.3L/L·d,8.1L/L·d,COD3000～5500mg/L800～3000mg/L,HRT10.4h,、264.1、519.8、104.6mg/L,60.6%。,COD。3.3 (SEM)(TEM),(Methanosaeta),。1 LattingaG,vanVelson,AFM,etal.Useoftheupflowsludgeblanket(USB)reactorconceptforbiologicalwastewatertreat-ment.Biotechnol.Bioeng.,1988,22:699～7342 LattingaG.Anaerobicdigestionandwastewatertreatmentsys-tems.AntonicvanLeeuwenhock.1995,67:3～283 SchmidtJE,AhringBK.Granularsludgeformationinupflowanaerobicsludgeblanket(UASB)reactors.BiotechnologyandBioengineering,1996,49:229～2464 YangXiushan,ZhouMengjin.Theanaerobicdigestionofsoy-beancheesewastewater.ChinaEnvironmentalScience,1991,2(2):46～51 ,19461。。。:“”();“”();“”();“”()─。40。。(EPA)JamesPorter,1996～1997,Keys3。,,。,。(FIU)LaurieRichardson,EPA。Keys9,4。。BigPineKeyBri-anLaPoint,,。FIURonJones,,,KeyWest,25000、670、11000。,。 《WaterEnvironmental&Technology》,April,28(1998)3594 :(Methanosarcina)—