固定化啤酒废酵母对Cr的吸附张帅

3　3环境工程学报Vol.3,No.320093ChineseJournalofEnvironmentalEngineeringMar.2009Cr(Ⅵ)张　帅1　程　昊2(1.,526061;2.,545006)　　2%1%,Cr(Ⅵ)。,Cr(Ⅵ)、pH、、Cr(Ⅵ)。Cr(Ⅵ):pH2,Cr(Ⅵ)100mg/L,2g/L,90min。Cr(Ⅵ)96.8%。Pd2+Cr(Ⅵ)。1mol/LCr(Ⅵ)3h,93.6%。　　　　(Ⅵ)　X172　　　A　　　1673-9108(2009)03-0489-04BiosorptionofCr(Ⅵ)byimmobilizeddiscardedbrewersyeastZhangShuai1　ChengHao2(1.SchoolofChemistryandChemicalEngineering,ZhaoqingUniversity,Zhaoqing526061;2.DepartmentofBiologicalandChemicalEngineering,GuangxiUniversityofTechnology,Liuzhou545006)Abstract　Discardedbrewersyeastwasentrappedinamixtureof2%sodiumalginateand1%gelatin,propertiesonbiosorptionofCr(Ⅵ)byimmobilizeddiscardedbrewersyeastwerestudied.Itisshownthatfactorssuchasadsorptiontime,initialpH,immobilizedbiamassconcentration,Cr(Ⅵ)initialconcentration,co-ions,etc.affectadsorptionprocess.Theoptimaladsorptionconditionsforimmobilizeddiscardedbrewersyeastwereasfollows:pH2,Cr(Ⅵ)initialconcentration100mg/L,immobilizedbiamassconcentration2g/L,adsorptiontime90min.Undertheseconditions,thebiosorptionefficiencyofCr(Ⅵ)was96.8%.Co-ions(e.g.Pd2+etc.)inhibitedtheCr(Ⅵ)adsorptionbyimmobilizeddiscardedbrewersyeast.93.6%ofCr(Ⅵ)couldbere-coveredfromimmobilizeddiscardedbrewersyeastadsorbedCr(Ⅵ),desorbedwith1mol/LHClfor3hours.Keywords　discardedbrewersyeast;immobilization;biosorption;chromium(Ⅵ):2008-05-25;:2008-07-04:(1978～),,,。E-mail:[e-mail]　　[1,2]。、,。,,、[3,4]。,[5,6],,[7]。,,,。(Ⅵ),[8]。(Ⅵ)。,(Ⅵ)、pH、Cr(Ⅵ),,(Ⅵ)。1　1.1　　1.1.1　菌体制备。2～3,3500r/min10min,,80℃,3,。1.1.2　固定化菌体制备[9]-:2g1g,,1.2g100mL,4%CaCl2,24h,。:,。(PVA)-:2%PVA1%,。--PVA:、PVA1%,。2～3mm。1.2　1.2.1　主要试剂K2Cr2O7,,Cr(Ⅵ)1000mg/L,。1.2.2　主要仪器BS-1E,WFX-IF2B2,TDL-40B,pHS-29A。1.3　Cr(Ⅵ)50mL150mL,(),pH,,,(25℃,150r/min);3500r/min10min,1mLCr(Ⅵ),3,qCr(Ⅵ)η[10]:q(mg/g)=V(C0-Ct)m(1):η(%)=(C0-Ct)C0×100%(2):V(L);C0Cr(Ⅵ)(mg/L);CttCr(Ⅵ)(mg/L);m(g)。1.4　Cr(Ⅵ),,10mL,3h,,,Cr(Ⅵ),:(%)=Cr(Ⅵ)/Cr(Ⅵ)×100%[11]。1.5　Cr(Ⅵ)[12]。2　2.1　90min,pH2,Cr(Ⅵ)100mg/L,2g/L,。(1),2%1%,2%1%。1　Cr(Ⅵ)Table1　AdsorptionofCr(Ⅵ)byvariousimmobilizedagentandimmobilizedbiomass(%)2%+1%38.122%+1%+93.242%36.082%+86.261%+2%PVA32.471%+2%PVA+83.651%+1%+1%PVA32.111%+1%+1%PVA+81.982.2　2.2.1　吸附时间的影响Cr(Ⅵ)100mg/L,pH2,2g/L,Cr(Ⅵ),1。1　Cr(Ⅵ)Fig.1　EffectofadsorptiontimeonCr(Ⅵ)adsorption1,5min,Cr(Ⅵ),5min75.2%,4903　:Cr(Ⅵ),90min,96.8%,Cr(Ⅵ),90min。,Cr(Ⅵ),。2.2.2　起始pH的影响Cr(Ⅵ)100mg/L,2g/L,90min,pHCr(Ⅵ),2。2　pHCr(Ⅵ)Fig.2　EffectofinitialpHonCr(Ⅵ)adsorptionCr(Ⅵ)H2CrO4、HCrO-4、CrO2-4Cr2O2-7,pHCr(Ⅵ),Cr(Ⅵ)7.69×10-3mol/L,pH1,H2CrO4,1pH6.5,HCrO-4,H2CrO4CrO2-4,pH6.5,Cr(Ⅵ)CrO2-4[13]。Cr(Ⅵ)100mg/L(2×10-3mol/L),2,pH2～3,Cr(Ⅵ),Cr(Ⅵ)HCrO-4。pH,,Cr(Ⅵ)。pH,,。Cr(Ⅵ)pH2～3,pH。2.2.3　固定化菌体浓度的影响Cr(Ⅵ)100mg/L,pH2,90min,Cr(Ⅵ),3。3,,,,[14];,2g/L,96.8%,,3　Cr(Ⅵ)Fig.3　EffectofimmobilizedbiamassconcentrationonCr(Ⅵ)adsorptionCr(Ⅵ),。2.2.4　Cr(Ⅵ)起始浓度的影响pH2,2g/L,90min,Cr(Ⅵ)Cr(Ⅵ),4。4　Cr(Ⅵ)Cr(Ⅵ)Fig.4　EffectofCr(Ⅵ)initialconcentrationonCr(Ⅵ)adsorption4,Cr(Ⅵ),Cr(Ⅵ),。Cr(Ⅵ),Cr(Ⅵ)100mg/L,Cr(Ⅵ),Cr(Ⅵ),96.8%,,48.4mg/g;Cr(Ⅵ),,,;Cr(Ⅵ),,,96mg/g;,Cr(Ⅵ)。49132.2.5　共存离子的影响pH2100mg/LCr(Ⅵ),Pd2+、Pb2+、Cu2+、Au3+Al3+(100mg/L),,2g/L。(2),Cr(Ⅵ),Cr(Ⅵ)。2　Cr(Ⅵ)Table2　EffectofCo-ionsonCr(Ⅵ)adsorptionbyimmobilizedbiomassCr(Ⅵ)(%)(%)96.80Pd2+82.414.9Pb2+74.523.0Cu2+73.324.3Au3+62.735.2Al3+57.640.52.3　Cr(Ⅵ),(3),、,,1mol/L,93.6%,H+。Cr(Ⅵ),,,,-。3　Table3　DesorptionofCr(Ⅵ)adsorbedbyimmobilizedbiomass(%)(%)(0.5mol/L)82.5NaOH(1mol/L)8.6(1mol/L)93.612.4(0.5mol/L)59.1NaCl(0.5mol/L)3.9(9%)20.5NH4Cl(0.5mol/L)15.33　　2%1%,Cr(Ⅵ):pH2,Cr(Ⅵ)100mg/L2g/L,90min,Cr(Ⅵ)96.8%。,。Cr(Ⅵ),1mol/L,。Cr(Ⅵ)Cr(Ⅵ)。[1]KratochvilD.,VoleskyB.Advancesinthebiosorptionofheavymetals.TrendsinBiotechnol.,1998,16(7):291～300[2]AkkalogluI.,ButterT.J.Screeningofvarioustypesbio-massforremovalandrecoveryofheavymetals(Zn,Cu,Ni)bybiosorption,sedimentationanddesorption.WaterScienceandTechnology,1998,38(6):269～277[3]BuxF.,AtkinsonB.,KasanH.C.Zincbiosorptionbywasteactivatedanddigestedsludges.WaterScienceandTechnology,1999,39(10):127～130[4]PuranikP.R.,PaknikarK.M.Biosorptionofleadandzincfromsolutionsusingstreptoverticilliumcinnamoneumwastebiomass.JournalofBiotechnology,1997,55(2):113～124[5]WangJ.L.Biosorptionofcopper(Ⅱ)bychemicallymodifiedbiomassofSaccharomycescerevisiae.ProcessBi-ochemistry,2002,37:847～850[6],,..,2002,15(2):26～29[7],,..,2001,21(2):30～32[8]..:,1989.139～162[9],,,.Pt4+.(),2003,42(6):800～804[10],.Cr(VI).,2007,29(3):79～83[11],,,.R08Pd2+.,2000,42(6):700～705[12]..,2005,15(7):832,866[13]CabatinganL.K.,AgapayR.C.,RakelsJ.L.,etal.Potentialofbiosorptionfortherecoveryofchromateinin-dustrialwastewaters.Ind.Eng.Chem.Res.,2001,40:2302～2309[14],,.Pb2+.(),2007,28(3):98～102492