固定化啤酒酵母菌吸附Pb2的研究王立娜

Pb2+Pb2+20g/L2%PVA+1%SA2:1CaCl23%(W/V)4hpH4.0Pb2+0.2413mmolL170%90%Langmuir0[e-mail]130022ResearchonAdsorptionofImmobilizedBeerYeastonPb2+WANGLinaZHAORuixueZHENGXiaoqiuLIUShumei(SchoolofChemistryandEnvironmentalEngineeringChangchunUniversityofScienceandTechnologyChangchun130022)AbstractImmobilizedbeeryeastisagoodbiologicaladsorbentonPb2+.TheoptimalimmobilizedconditionofBeerYeastwasdeterminedbyorthogonaltest.Atthesametimewediscussedtheinfluenceofdifferentfactorsonbiologicalabsorption.Theresultsshowedthattheoptimalimmobilizedconditionsofbeeryeastwereasfollow:theamountofbeeryeastwas20g/LtheratioofthePVAandSAembeddingagentswas21theconcentrationofCaCl2was3%(W/V)theimmobilizedtimewas4h.pHwas4.0thePb2+initialconcentrationwas0.2413mmolL1theabsorptionefficiencyofuntreatedyeastimmobilizedwasupto70%afterthreetimestheelutionefficiencywasabove90%respectively.Theprocessofbio-logicalabsorptionisaccordwiththeLangmuirmodelandcorrelationcoefficientwas0.9976.Moreovertheexperimentalre-sultswerefittoLagergrensecondaryadsorptionefficiencyequation.Keywordsimmobilizationbiologicalabsorptionleadionbeeryeast-1100mgL123-78-Pb2+Pb2+11.11.1.132120093JournalofChangchunUniversityofScienceandTechnologyNaturalScienceEditionVol.32No.1Mar.20092818h4000r/min601.1.2PHS-25pHBS-1ETGL-16G10ml721E1.1.30.7239mmolL10.1mgmL13%CaCl2(SA)(MJ)(PVA)1.21.2.13%CaCl23PVA-SA2%PVA1%SAPVA-SA-MJPVASAMJ1%SA2%SASA-MJ2%SA1%MJMJ4%MJ2mm381.2.2Pb2+lmolL1NaOHHC1pHpH180r/min100mL721E510nmPb2+Pb2+(%)=(CiCf)/Ci×100%(mg/g)=(CiCf)×V/WCiCf(molL1)V(L)W(g)Pb2+Pb2+=Pb2+/Pb2+×100%22.1Pb2+l1Tab.1Betweancomparisonsseveralkindsoffixedcar-riersPVA-SAMJ-SASAPVA-SA-MJMJ(%)[8]92.6588.8390.5685.2950.52lPVA-SAPb2+92.65%PVA-SASAMJ2.2Pb2+CaC12232Tab.2TableoffactorleveldesignA(g/L)BPVA(g)/SA(g)CCaCl2(%)(W/V)D(h)123102062:11.5:1.51:234524633.72Pb2+1161A2B1C1D220g/L(PVA(g)/SA(g))2:1CaC123%4h2.3Pb2+2.3.14Tab.4Effectofdifferentpretreatmentmyceliumonbi-osorption(A)(%)PVA-SAMJ-SASAPVA-SA-MJMJ0.2770.3010.2740.3050.3060.2890.2490.2490.2480.3530.3340.3280.7630.7580.71189.9287.7492.6587.3887.2988.8392.4692.4690.5686.8484.7585.2945.8046.2550.520.1molL1HCI0.1molL1NaOH60min601.2pH4.0Pb2+0.2413mmolL120g/L28180r/min3h44Pb2+PVA-SAPb2+92.65%9H+2.3.2pHpH0.2413mmolL1Pb2+pH3.03.54.04.55.05.520g/L180r/min283h11pHFig.1InfluenceofPHvalueonbiosorption1pH3.54.589%pH4.091.92%pHH+Pb2+pH-COOH-OHpHpH3.54.5pHpH2.3.3Pb2+Pb2+0.09650.24130.72391.20652.4136.0325mmolL1pH4.020g/L180r/min283h3Tab.3AnalysisoforrthogonaltestresultABCD123456789K1K2K3k1k2k3R268.22275.12263.9689.4191.7187.993.72274.13267.86265.3291.3889.2988.442.94275.49265.41266.4191.8388.4788.803.36263.59272.31271.4087.8690.7790.472.91A1B1C1D1A2B1C2D3A3B1C3D2A1B3C3D3A3B2C1D3A1B2C2D2A2B3C1D2A3B3C2D1A2B2C3D191.3892.9289.8388.0290.4788.8393.6483.6688.56A2B1C1D2200916222Pb2+Fig.2EffectofPb2+concentrationonbiosorption2Pb2+Pb2+Pb2+Pb2+2.3.4Pb2+28Pb2+LangmuirFreudlich55LangmuirFreudlichTab.5ValueoflangmuirandFreudlichmodelparameter(R2)LangmuirFreudlichKL=0.025qmax=32.15KF=0.903n=1.2450.99760.9931y=1.2225x+0.0311y=0.8031x0.0441LangmuirFreudlichPb2+Langmuir2.3.56Pb2+11636Tab.6Dynamicsmodeloffixedsaccharomycetesadsorptiononleadion2913014.606×1044.606×1041.418(3.580)1.510(3.530)0.67560.82830.20720.33352.2302.23911T/KK1/(min)qe/(mgg1)R2K2/(gmg1min1)qe/(mgg1)R262.4Pb2+pHPb2+Pb2+Pb2+HCl60min77Tab.7Influenceofelutionagentconcentrationonelu-tingefficiencyHCl(molL1)Pb2+(%)1230.10.51.037.5297.3298.4571.0molL1HClH+H3O+PbCl421.0molL1HCl1.0molL1HClPb2+515306090min50.23%71.51%85.67%98.41%98.73%2.5Pb2+86.10mgL1pH2.4Pb2+7.08mgL191.78%88370%90%48Pb2+Tab.8Effectoffixedmyceliumreutilizationonbiosor-ptionefficiencyofPb2+%%91.7883.6470.9044.8298.4596.8393.4788.26320g/L(PVA/SA)2:1CaCl23%(W/V)4hpH4.0Pb2+0.2413mmolL120g/L283h91.78%1.0molL1HCl98.45%70%90%Langmuir0.9976Lagergren1J)20021634649.2J.20041411519.3WiliamsCJAderholdDEdyveanRGJ.Comparisonbe-tweenbiosorbentsfortheremovalionsfromaqueoussol-utionJ.WaterResearch19983112162244.J.19991611925.5.J.19981947275.6.M.20027.7.M.20025.8.J.20052862224.9DrakeLRLinSRaysonGD.ChemicalmodificationandmetalbindingstudiesofDaturainnoxinJ.Environmen-talScienceandTechnology1996301110114.2009164871FanucciL.Asinewavedigitalsynthesizerbasedonaqua-draticapproximationJ.ProcIEEEInternationalFCSa-ndPDAexhibition2001806810.2.FPGADDSJ.20071726366.3ChrisDFharrisDirectdigitalsynthesisSomeOptionforFPGAImplementationJ.ProcofSPIEReconfigurabletechnologyFPGAsforComputingandApplication.Bo-stonMassachusetts19993844210.4.VLSIM.2003.