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———1李正魁, 2濮培民, 2胡维平, 2胡春华, 1张卫东, 1朱佳廷(1,210014;2,210008):2001-07-20:[CI1＊-CT93-0094(DG12HSMU)];(BS99021):(1959-),,,,,。 :(INCB)(PEEN),INCB,PEENINCB,PEEN-INCBINCB。,INCB,INCBPEEN,(MPN)MPN(P0.005);PEEN-INCB,INCBINCBMPN(P0.01)。-。:;;;:X172 :A :1000-4440(2001)04-0248-05TechniquesofImmobilizedBacteriaandItsApplicationinPhysico-ecologi-calEngineering—AquaticEcosystemRemediationwithImmobilizedNitro-genCyclingBacteria1LIZhengqui, 2PUPeimin, 2HUWeiping, 2HUChunhua, 1ZHANGWeidong, 1ZHUJiating(1InstituteofApplicationofAtomicEnergyinAgriculture,JiangsuAcademyofAgriculturalSciences,Nanjing210014,China;2NanjingInstituteofGeographyandLimmnology,AcademiaSinica,Nanjing210008,China) Abstract:Thereleaseexperimentsofimmobilizednitrogencyclingbacteria(INCB)wereconductedinthephysico-ecologicalengineering(PEEN)experimentplotinHongfenghuLakeofGuizhouprovince.Theamountoffourtypesofnitrogencyclingbacteriaincludingammonifyingbacteria,nitrobacteria,nitrosobateriaanddenitrifyingbacteriawereinvestigated.Itcouldbefoundfromtheresultsthat:①INCBreleasedremarkablyinPEENexperimentplotusingtechniquesofINCB,andthemostprobablenumber(MPN)ofnitrogencyclingbacteriainthewaterfromallthePEENexperimentplotweresignificantlyhigher(P0.005)thanthatinthewaterfrominletsite;②MPNofnitrogencyclingbacteriaaroundrootzoneofaquaticplantsinplotsusingINCBtechniqueweremore(P0.01)thanthatintheplotswithoutusingINCBtechniquesignificantly;③thedesignofPEENexperimentplotaffectedthereleaseofnitrogencyclingbacterianoticeably.Keywords:immobilizednitrogencyclingbacteria(INCB);nitrogencycling;aquaticecosystem;physico-ecologicalengi-neering(PEEN) ,,,。、、,,248(JiangsuJ.ofAgr.Sci.),2001,17(4):248～252[1,2]。[3,4],、、,。,,,,。,-[5～7]。[8,9](ImmobilizedNitrogenCyclingBacteria,IN-CB)INCB,。。1 1.1 -INCB,26°24′37″～26°34′17″,106°27′00″～106°34′17″,,4km,31.5km,,。,。“”、“”、“”3。,,、,。200024。,,5600m2,,。(PEEN)425m22100m2,。(19991220002),5600m2PEEN128.3m2,30kgINCB、;1100m2INCB,IN-CB。,、、。1.2 (、、、)(PhysicoEcologicalEngineering,PEEN),[10],,、、[11,12],pH,1.03×105Pa20min,(28±1)℃30d、15d,、、,。1.3 (2-Hy-droxyethylacrylate,HEA)β-(2-Hydroxyethylmethylacrylate,HEMA),,、60Co-γ,[13]。1.4 ,28℃24h3,,4℃550g20min、、,28℃24h、48h,、、,,。1.5 PEEN-INCB PEEN-INCB,(MostProbableNumber,MPN):、、,pH,150mm、14mm,30mm、5mm,,1.03×105Pa20min。3～410,1ml,5,(28±1)℃,、15d,30d。249:———--+20%、Nessler()、Griess()、、-,、。MPN,n(MPN)(L-1):n(MPN)=MPN×[10(ml)÷(ml)]2 2.1 (INCB)PEEN-INCB,PEEN,,、“”,,,。PEEN,,INCB,PEEN,INCB,,,。2.2 -INCB PEEN-INCB,INCBPEEN。,F,MPNMPN(P0.01)。。,、、10cm、30cm,MPNMPN(P0.005)。PEENMPN,MPNMPN(P0.005)。,INCBPEEN,INCB10cm,MPN(P0.001)(1)。,。a:;b:INCB10cm;c:INCB30cm;d;e:;f:;g:;h:IN-CB;i:INCB;j:;k:;l:;m:INCB15m;n:。a:imobilizednitrogencyclingbacterium(INCB);b:10cmfromINCB;c:30cmfromINCB;d:inlet;e:outlet;f:leftupperopenarea;g:rightupperopenarea;h:rootareawithoutINCB;i:rootareawithINCB;j:leftloweropenarea;k:rightloweropenarea;l:upperdirectflowingarea;m:15mfromINCB;n:HongfenghuLake. 1 Fig.1 ReleaseofINCBindifferentexperimentplotsinPEEN2.3 -INCB ,-INCB,INCB、、46,INCB,1～2,、MPN(P0.01)(2)。250 2001174 2 Fig.2 EffectofbacteriareleasedbyINCBonbacteriaaroundtheaquaticmacrophytes2.4 -INCBINCB ,PEENPEEN,,。,,(3)。,INCB,PEENMPNMPN。,、、、MPN,、A:;B:。A:upperopenarea;B:loweropenarea.3 PEENFig.3 DistributionofINCBreleaseinPEENexperimentplotMPN(P0.05)。INCB,INCB,-。3 ,,-,-。,PEEN-INCB,,-,-。--,,、()-。PEEN,、、,INCB-,INCB,-,--251:———,PEEN-,。,,-,NH3。,。PEEN--。,PEEN,,-,。。:[1] ,,,.[J].,1993,17(2):150～155[2] .[M].:,1994.26～33[3] ,.(Potamogetoncrispus)[J]..1991,11(4):411～416[4] ,,,.(Potamogetoncrispus)[J].,1994,14(2):168～173[5] RheinheimerG.AquaticMicrobiology(ThridEdition)[M].NewYork:JohnWileyandSons,1985[6] MatulewichVA,FinsteinMS.Distributionofautotrophicnitrifyingbac-teriainapollutedriver(thePassaic)[J].AppliedandEnvironmentalMicrobiology,1978,35(1):67～71[7] NorthupRR,ZengshouYU,RandyA,etal.Polyphenolcontrolofnitro-genreleasefrompinelitter[J].Nature,1995,377(21):227～229[8] ,.[J].,2000,12(4):321～326[9] ,.[J].,2000,16(2):115～117[10]《》.[M].:,1993[11]MatulewichVA,StromPF,FinsteinMS.Lengthofincubationforenu-meratingnitrifyingbacteriapresentsinvariousenvironments[J].ApplMicrobiol.1975,29(2):265～268[12],,.[M].:,1996[13],.[J].,2000,16(1):37～40252 2001174