固定化硝化菌在养鱼废水处理中的应用王光玉

4110200910JOURNALOFHARBININSTI[e-mail]2.15009060L.46d8mg/L·d.PVA、15d.1h0.6mg/L100%.0.5mg/L10～90min、PVA、PVA15min、18min22min70g/m3·d、58g/m3·d48g/m3·d..P76A0367－6234200910－0078－04Removalofammonium－NfrommarinerecirculatingaquaculturebyimmobilizednitrifiersWANGGuang-yu12MAFang2LIWei-guo12BIJian-pei12MAMei-rong11.OceanCollegeHa[e-mail]nWaterResourceandEnvironmentSchoolofMunicipalandEnvironmentalEngineeringHarbinInstituteofTechnologyHarbin150090ChinaAbstractMarinenitrobacterswereenrichmentedacclimatedandusedtodenitrificateofmarinerecirculatingaquaculturebyimmobilizationinanaquariumof60L.After46dtheammoniaoxidationrateandthenitriteoxidationratewerebothover8mg/L·dbyadjustingtheammoniumconcentrationinappropriatecondi-tion.Anadsorptionexperimentwascarriedoutfor15dwithtwokindsofPVAbeadsandtheactivatedcarbonbeadsfilledinthreeplasticcolumnsrespectively.Andexperimentstoevaluatetheefficiencynitrificationofthreefixedpackedbedcolumnwereconducted.ResultsshowthatwithahydraulicretentionHRTof1handtheinfluentammoniaconcentrationlessthan0.6mg/Lallthethreereactorscanachievetheefficiencyof100%.WhenHRTissetintherangeof10－90minthehighestammonia－Nremovalratesof70g/m3·d58g/m3·dand48g/m3·darefiguredoutfortheactivatedcarbonthePVAbeads2mmandthePVAbeads4mmreactorsrespectivelywiththeoptimalHRTof15min18minand22min.KeywordsnitrifiersimmobilizationrecirculatingaquaculturesystemactivatedcarbonPVAfixedpackedbedreactor2008－03－29.50778052HC2008013.1972—1963—.1.2.3－5..11.1460L.40L.0.5g/L0.5mg/L1、2、4、6mg/L8mg/L.pH1mol/LNa2CO37.5～8.51.02025±2℃DO5mg/LCO2.1.2PVA2000～3000、Al2SO43、H3BO4、CaCO33－4.PVA1.1%123401.71.71.70000.3001.71.78.38.38.38.3PVA30～35℃PVA、.4mm、2mm、.0～4℃48h3.160～250℃.4.1.3.3.6cm30cm3PVA、.2.2/cm3310310310/cm3130130130/mm24h=2d=1/31050388181250/cm23900195051021mg/LDOCO2pH7.5～8.525℃.35m3/m2·d0.6kg/m3·d.15d3d3、、.1.进水缸循环水泵活性炭柱PVA小球柱PVA大球柱11.4《》HANNAHI98128pHHANNAHI9142DO.1.53·97·10.1h1dpH、、、.1.6.0.5mg/L10～150min.22.1pH..46d8mg/L·d2..、..氨氮添加的氨氮亚硝态氮硝态氮总氮添加的总氨氮109876543210159131721252933374145t/d180160140120100806040200水体中氨氮质量浓度和添加的氨氮质量浓度/（mg·L-1）亚硝酸盐、硝酸盐、总氮和总添加氨氮/（mg·L-1）22.21h1d.pH7.53pH7.1～7.3.NH4+－NNO2－－N3.3.330.6mg/L0.5mg/L.0.60.50.40.30.20.10进水活性炭柱出水PVA大球柱出水PVA小球柱出水1234567t/d籽(氨氮)/(mg·L-1)32.30.5mg/L10～150min。4、5、PVA、PVA4、5.20min100%.15min70g/m3·d90%PVA18min58g/m3·d95%PVA22min48g/m3·d100%。氨氮去除速率/(g·m-3·d-1)8070605040302010氨氮去除速率氨氮去除效率050100150120100806040200氨氮去除率/%停留时间/min43·08·41605040302010020406080100氨氮去除速率氨氮去除率120100806040200氨氮去除率/%停留时间/min氨氮去除速率/（g·m-3·d-1）(a)PVA小球柱5040302010氨氮去除速率氨氮去除率120100806040200氨氮去除率/%020406080100停留时间/min氨氮去除速率/（g·m-3·d-1）(b)PVA大球柱5PVA、.6.3－57－8.5PVAρNH4+－N=45mg/LNH4+－N82.5%.Shan7.TP8.Kim3、RHT0.3hNH4+－N82g/m3.9Balderston10、NO3－－N.Chen11TOC/NO3－－N1.、.1GUTIERREZ－WINGMTMALONERF.BiologicalfiltersinaquacultureTrendsandresearchdirectionsforfreshwaterandmarineapplicationsJ.AquacultEng20063163－171.2CAOLWANGWYANGYetal.Environmentalim-pactofaquacultureandcountermeasurestoaquaculturepollutioninChinaJ.EnvSciPollutRes2007147452－462.3KIMSKKONGILEEBHetal.Removalofammo-nium－NfromarecirculationaquaculturalsystemusinganimmobilizednitrifierJ.AquacultEng200021139－150.4SEOAJKJUNGIHKIMMRetal.NitrificationperformanceofnitrifiersimmobilizedinPVApolyvinylalcoholforamarinerecirculatingaquariumsystemJ.AquacultEng200124181－194.5.J.1999273351－354.6METAXAEDEVILLERGPAGANDPetal.HighratealgalpondtreatmentforwaterreuseinamarinefishrecirculationsystemWaterpurificationandfishhealthJ.Aquaculture200625292－101.7SHANHOBBARDTP.Ammoiaremovalfromprawnaq-uaculturewaterusingimmobilizednitrifyingbacteriaJ.ApplMicrobiolBiotechnol200157791－798.8.J.2004284419－424.9JAAPVANRYOSSITHAROLDJetal.Denitrifica-tioninrecirculatingsystemsTheoryandapplicationsJ.AquacultEng200634364－376.10BALDERSTONWLSIEBURTHJ.NitrateremovalinclosedsystemaquaculturebycolumnardenitrificationJ.ApplandEnvironmentalMicrobilogy1976326808－818.11CHENSLINGJBLANCHETONJP.NitrificationkineticsofbiofilmasaffectedbywaterqualityfactorsJ.AquaculturalEngineering200634179－197.·18·10