固定化藻菌去除淡水养殖废水氨氮效果及模型拟建邹万生

邹万生1,2,张景来2,刘良国1,邓武军3　(1.,415000;2.,100872;3.,410000)　[目的]研究固定化藻菌去除淡水养殖废水氨氮效果及模型拟建。[方法]利用固定化藻菌(ABI)处理淡水养殖废水中的氨氮,采用多因子正交试验得到温度(T)、pH、光照强度(I)、溶解氧(DO)和填充率五因子与氨氮去除率(AR)之间的关系数据,拟建五因子与AR关系模型。[结果]五因子对AR均具有显著影响,最佳去氨氮组合为:温度30℃、pH值7.0、光照强度6000lx、溶解氧5.0mg/L、填充率10%。根据试验数据拟建的方程模型决定系数R2=0.8648、相伴概率P0.05,对AR的模型预测值与实际测值进行样本T-检验,结果表明总体均值差异显著值sig.为0.978(P0.05),模型预测值与实际测值数据组无显著性差异,模型具有较高拟合度。[结论]为实现养殖废水中氨氮去除或浓度降低提供了参考依据。　固定化藻菌;养殖废水;氨氮去除率;拟建模型　X703.1　　　A　　　0517-6611(2010)23-12650-02EffectResearchofImmobilizedAlgae-bacteriaRemovalAmmoniaNitrogenofAquacultureWastewaterandProposedModelZOUWan-shengetal　(LifeSciencesofArtsandsciencesofHunanUniversity,Changde,Hunan415000)Abstract　[Objective]Theaimwastoresearchtheeffectofimmobilizedalgae-bacteriaremovalammonianitrogenofaquaculturewastewaterandmodelconstruction.[Method]Immobilizedalgaebacteria(ABI)wasappliedtoremoveammoniaoffreshwateraquaculturewastewater.Fivetactoroftemperature(T),pH,lightintensity(I),dissolvedoxygen(DO)andfillingratewereusedforMulti-factororthogonaltestandrelateddatabetweenammoniaremovalandfivefactorswasreceived,thenrelationsmodelbetweenthefivefactorandnitrogenremovalwases-tablished.[Result]Theresultsshowedthatfive-factorshadsignificanteffectonAR,andthebestcombinationsforremovingARwastempera-ture30℃,pH7.0,lightintensity6000lx,dissolvedoxygen5.0mg/Landthefillrate10%.Accordingtotheexperimentaldata,equationmodelwasproposedandcoefficientofdeterminationR2=0.8648,P0.05.SamplesT-testwasdonebetweenthemodelpredictionsandtheactualmeasuredvalues,andtheresultsshowedthatthesignificantdifferenceofoverallmeanvaluesig.(2-tailed)was0.978(P0.05),whichindicatedthattherewasnosignificantdifferencebetweenmodelpredictionsandtheactualmeasuredvalue,andmodelhadahighdegreeoffitting.[Conclusion]Thestudyprovidedareferenceforrealizingremovalammonianitrogenofaquaculturewastewaterandconcentrationde-cline.Keywords　ImmobilizedAlgae-bacteria;Aquaculturewastewater;Ammoniaremoverate;Proposedmodel　国家自然科学基金资助项目(NO.30972260)。　邹万生(1975-),男,湖南新化人,硕士,从事水污染控制技术研究。　2010-05-24　　(Algae-bacteriaImmobilized,ABI),、,[1-4]。、、、,、,[5-7]。,,,。Olguin[8],,,。,ABI,(Ammonia-removalRate,AR),、pH、、(AR)。1　1.1　1.1.1　。(Chlorellapyre-noinoidosa),;Kuhl[9];,。(),25℃、5000lx、12∶12。1.1.2　。,0.45μm,:CODcr310.8mg/L,(NTU)17.3mg/L,30.1mg/L,pH7.2。　　COD;;;pHpH。Kuhl,,。1.2　1.2.1　。[10-14](Mix-immobilization,MI):2500r/min,,;(2%SA+8%PVA),2%;,8,(2%CaCl2+4.5%H3BO3),3mm,CaCl24h。1.2.2　。(A)、pH(B)、(C)、(D)(E),,　马树梅　　况玲玲,JournalofAnhuiAgri.Sci.2010,38(23):12650-12652。L25(55)。1　Table1　TheorthogonaltestoffivefactorsLevelsFactors(A)∥℃TemperaturepH(B)pHvalue(C)∥lxLightintensity(D)mg/LDO(E)∥%Fillingrate206.020002.05256.540003.08307.060004.010357.580005.013408.0100006.015　　500ml,300ml,1mol/LHClNaOHpH;,12∶12;DO;;120r/min;24h,(AR)。2　2.1　　(AR)2,,AR97.0%,67.1%,。2,3。3,、pH、、(P0.05),,。2　Table2　Ammoniaremovalrateunderdifferentinitialconditions(A)∥℃TemperaturepH(B)pHvalue(C)∥lxLightintensity(D)mg/LDO(E)∥%Fillingrate∥%AR406.520005.0884.2207.540004.01086.7308.060002.01088.3207.0100005.01390.1357.560005.0894.5257.580006.0586.5256.060004.01391.8307.080004.01597.0407.5100002.01570.3258.020005.01588.7356.580002.01387.6208.080003.0888.5257.040002.0889.4406.080005.01086.3256.5100003.01094.2206.020002.0567.1306.540005.0590.5407.060003.0584.6307.520003.01390.8358.0100004.0582.7356.040003.01587.9306.0100006.0889.4206.560006.01590.7357.020006.01089.4408.040006.01378.62.2　　,,。,1AR,AR。,,AR。3　Table3　VarianceanalysisonfivefactorsandscalestatisticsFactors(A)TemperaturepH(B)pHvalue(C)Lightintensity(D)DO(E)Fillingrate0.0050.0200.0230.0070.012Significantvalue30℃7.0006000lx5.0mg/L10%Optimallevelvalue2.2.1　。ARpH、(T)、(I)、(D)(L)AR=f(pH,D,I,T,L)。2.2.2　。,。,(CF)[15]。pH、(T)、(I)、(D)(L),。AR,。AR,CF,CF=TαpHβIθDδLγ,α、β、θ、γ、δ,,SPSS。2.2.3　。2,,:α=0.201、β=-0.135、θ=0.147、γ=0.200、δ=0.190。CF=TαpHβIθDδLγ=T0.201pH-0.135I0.147D0.200L0.190。2,,,AR,AR。1。1　Fig.1　Regressionmodelbetweenammoniaremovalrateandsynthesisfactor1265138卷23期　　　　　　　　　　　　　　　　邹万生等　固定化藻菌去除淡水养殖废水氨氮效果及模型拟建　　1ARAR=36.54LnT1/5pH-0.138I0.144D1/5L0.197-29.87,R2=0.8648,P0.05,n=25。2.2.4　。9,,9AR。(2、5)。2、5,AR。9,10.12%,0.20%,94.08%。AR,t。,sig.0.254(P0.05),,sig.(2-tailed)0.978(P0.05),2　Fig.2　Linechartofnitrogenremovalratemodelpredictionval-ueandtheactualmeasuredvalue。t。5　Table5　ComparisonofammoniaremovalratemodelpredictionvalueandtheactualmeasuredvalueInfluencingfactors∥℃TemperaturepHpHvalue∥lxLightintensity∥mg/LDO∥%Fillingrate∥%Modelpredictionvalue∥%Actualmeasuredvalue∥%Relativeerror∥%Meanrelativeerror227.430004.110.085.8786.040.20267.560005.85.588.3186.072.61266.390003.29.088.8694.015.47387.470002.214.091.5889.062.83346.550002.312.587.7387.100.734.08316.040005.58.592.3589.013.76376.420004.09.082.6384.071.71266.840002.08.879.2089.5010.12357.080005.210.093.4086.038.503　(ABI),AR(T)、pH、(I)、(D)(L),AR=36.54LnT1/5pH-0.138I0.144D1/5L0.197-29.87。ABI,(T)、pH、(I)、(D)(L),,30℃、pH7.0、6000lx、5.0mg/L、10%。,。,AR,,。[1]TRAVIESOL.Experimentsonimmobilizationofmicroalgaefornutri-entremovalinwastewatertreatment[J].BioresourceTechno,1996,55(3):181-186.[2]TAMNFY,WONGYS.Effectofimm