ResearchreviewpaperBiologicalnitrateremovalfromwaterandwastewaterbysolid-phasedenitrificationprocessJianlongWanga,b,c,⁎,LibingChua,caCollaborativeInnovationCenterforAdvancedNuclearEnergyTechnology,INET,TsinghuaUniversity,Beijing100084,PRChinabStateKeyJointLaboratoryofEnvironmentSimulationandPollutionControl,TsinghuaUniversity,Beijing100084,PRChinacBeijingKeyLaboratoryofRadioactiveWasteTreatment,TsinghuaUniversity,Beijing100084,PRChinaabstractarticleinfoArticlehistory:Received28May2016Receivedinrevisedform1July2016Accepted6July2016Availableonline7July2016Nitratepollutioninreceivingwatershasbecomeaseriousissueworldwide.Solid-phasedenitrificationprocessisanemergingtechnology,whichhasreceivedincreasingattentioninrecentyears.Itusesbiodegradablepolymersasboththecarbonsourceandbiofilmcarrierfordenitrifyingmicroorganisms.Avastarrayofnaturalandsynthet-icbiopolymers,includingwoodchips,sawdust,straw,cotton,maizecobs,seaweed,bark,polyhydroxyalkanoate(PHA),polycaprolactone(PCL),polybutylenesuccinate(PBS)andpolylacticacid(PLA),havebeenwidelyusedfordenitrificationduetotheirgoodperformance,lowcostandlargeavailablequantities.Thispaperpresentsanoverviewontheapplicationofsolid-phasedenitrificationinnitrateremovalfromdrinkingwater,groundwa-ter,aquaculturewastewater,thesecondaryeffluentandwastewaterwithlowC/Nratio.Thetypesofsolidcarbonsource,theinfluencingfactors,themicrobialcommunityofbiofilmattachedonthebiodegradablecarriers,thepotentialadverseeffect,andthecostofdenitrificationprocessareintroducedandevaluated.Woodchipsandpolycaprolactonearethepopularandcompetitivenaturalplant-likeandsyntheticbiodegradablepolymersusedfordenitrification,respectively.Mostofthedenitrifiersreportedinsolid-phasedenitrificationaffiliatedtothefamilyComamonadaceaeintheclassBetaproteobacteria.ThemembersofgeneraDiaphorobacter,AcidovoraxandSimplicispiraweremostlyreported.Infuturestudy,moreattentionshouldbepaidtothesimultaneousre-movalofnitrateandtoxicorganiccontaminantssuchaspesticideandPPCPsbysolid-phasedenitrification,totheelucidationofthemetabolicandregulatoryrelationshipbetweendecompositionofsolidcarbonsourceanddenitrification,andtothepost-treatmentofthemunicipalsecondaryeffluent.Solid-phasedenitrificationprocessisapromisingtechnologyfortheremovalofnitratefromwaterandwastewater.©2016ElsevierInc.Allrightsreserved.Keywords:BiologicaldenitrificationNitrogenremovalDenitrifyingbacteriaWatertreatmentWastewatertreatmentContents1.Introduction..............................................................11042.Solid-phasedenitrification:principleandcharacterization.........................................11043.Solidcarbonsourcecommonlyusedfordenitrification...........................................11043.1.Typesofsolidcarbonsourcesandtheirdenitrificationrate......................................11043.2.Effectofphysicalandhydraulicproperties.............................................11064.Factorsinfluencingdenitrificationperformance..............................................11064.1.Temperature..........................................................11064.2.Dissolvedoxygenconcentration..................................................11074.3.HRT(superficialhydraulicvelocity)andothers...........................................11075.Applicationofsolid-phasedenitrification.................................................11075.1.Nitrateremovalfromdrinkingwaterandgroundwater.......................................11075.2.Denitrificationforrecirculatingaquaculturesystem.........................................11085.3.TreatmentofwastewaterwithlowC/Nratioandthesecondaryeffluent................................11086.Microbialcommunityofbiofilmattachedonbiodegradablecarriers.....................................11087.Thepotentialadverseeffectofsolid-phasedenitrificationandcostevaluation................................11097.1.LeachingofDOCandammoniumandnitriteaccumulation......................................1110BiotechnologyAdvances34(2016)1103–1112⁎Correspondingauthorat:TsinghuaUniversity,Beijing100084,PRChina.E-mailaddress:wangjl@tsinghua.edu.cn(J.Wang).©2016ElsevierInc.Allrightsreserved.ContentslistsavailableatScienceDirectBiotechnologyAdvancesjournalhomepage:(Ghafarietal.,2008).Nitrateisidentifiedasoneofthehazardouscontaminantsi