光合产氢膜曝气生物膜反应器耦合系统产氢与底物降解特性田鑫

111Vol．11No．120171ChineseJournalofEnvironmentalEngineeringJan．20172013EG1211842015－09－142015－11－031979—。[e-mail]*-1*11121.3100302.310030CQK01-PBＲ-MABＲ。PBＲPBＲ-MABＲ0.49mmol·L·h－1PBＲ-MABＲ、PBＲ-2MABＲsPBＲ-3MABＲs0.61、0.760.85mmol·L·h－1。MABＲ。pH。X172A1673-9108201701-0613-07DOI10.12030/j．cjee．201509122Improvinghydrogenproductionandsubstratedegradationinaphotobioreac-torcouplingwithamembrane-aeratedbiofilmreactorTIANXin1*SUNShaopeng1ZHOUChongbo1YUTieming121.HuadianElectricPowerＲesearchInstituteHangzhou310030China2.NationalEnergyDistributedEnergyTechnologyＲ＆DCenterHangzhou310030ChinaAbstractToenhancehydrogenproductionfromglucosemembrane-aeratedbiofilmreactorsMABＲswerecoupledwithaphotobioreactorPBＲinthisstudy．Duringatypicalfed-batchcyclethePBＲinoculatedwithＲhodopseudomonaspalustrisCQK01exhibitedahydrogenproductionrateof0.49mmol·L·h－1．Uponcou-plingwithonetwoorthreeMABＲsthehydrogenproductionratewasincreasedto0.610.76and0.85mmol·L·h－1respectively．Thisincreasewasmainlyduetotheconsumptionoflargeamountsoftheaccu-mulatedinhibitorybyproductandH+fromthePBＲeffluentbythebiofilmsattachedtothemembraneoftheMABＲs．Keywordsphotobioreactormembrane-aeratedbiofilmbioreactorhydrogenproductionratesubstratedegrada-tion、、、。41-4。5-6。、、、7。8、Ｒhodoseudomonaspalustris、、、pH。11、。photobioreactorPBＲ9-10。PBＲ11-13。、pH14-15。PBＲ。MABＲs16。、、、MABＲ。PBＲMABＲMABＲMABＲPBＲPBＲpHPBＲPBＲ。MABＲPBＲ-MABＲPBＲ-MABＲ、pHPBＲ-MABＲ、。11.11.2.PBＲ3.4.5.6.MABＲ1PBＲ-MABＲFig．1SchematicofPBＲ-MABＲsystem、、CQK-0112-13CQK-01。K2HPO4·3H2O1.0060g·L－1KH2PO40.5540g·L－1MgSO4·7H2O0.2000g·L－1FeSO4·7H2O0.0417g·L－1NH46Mo7O24·4H2O0.0010g·L－1ZnSO4·7H2O0.0010g·L－1NaCl0.2000g·L－1CaCl20.0100g·L－1CONH221.6670g·L－10.5000g·L－11.0000g·L－11mL·L－1。。10g·L－1。PBＲ12.1W·m－272h30℃。。pH0.1mol·L－1NaOHHCl7.0。1.2PBＲ-MABＲPBＲ16276.5mL。MABＲ50mm×50mm×20mm50mL。Teflon。。MABＲPBＲPBＲ1～3MABＲ4161-PBＲ-MABＲsPBＲ-MABＲ、PBＲ-2MABＲsPBＲ-3MABＲs。MABＲ。。。PBＲPBＲ25d。MABＲPBＲMABＲ。PBＲMABＲpHpH。MABＲPBＲ。1.0mL·min－110。1.335-17。、、、DionexIC-50004mmAS11-HC30℃。1.5mL·min－1。1mmol·L－140mmol·L－1。1mL50mL1mL。。pHS20K-pH。SC-3000。=ΔQH2ΔCglucose1=ΔQH2ΔT·A2=C0－C1C03ΔQH2mmolΔCglucosemmolΔThAm2C0mg·L－1C1mg·L－1。22.1MABＲVFAPBＲ18。MABＲVFA1PBＲ。1CQK-01PBＲ、。。1MABＲPBＲ、＞＞。PBＲ-MABＲ、PBＲ。CQK-01MABＲ。1MABＲ、。PBＲPBＲPBＲ。2.2PBＲPBＲ-MABＲVFAMABＲPBＲ-MABＲVFA2。2a～2dPBＲPBＲ-MABＲ5161114Table1VFAconcentrationsinthespentmediaofthePBＲandPBＲ-MABＲsystemsmg·L－1VFAPBＲPBＲ-MABＲPBＲ-2MABＲsPBＲ-3MABＲs76.4±3.494.4±9.882.3±9.385.0±9.7487.5±18.2328.8±7.2182.1±3.9159.0±22.2175.9±2.3195.0±2.1198.8±9.5186.9±6.51470.3±28.61109.8±23.1984.1±15.4314.1±22.92308.2±52.52090.8±66.01498.5±98.3735.0±12.49h≤0.1g·L－1MABＲ。aPBＲbPBＲ-MABＲcPBＲ-2MABＲsdPBＲ-3MABＲs2PBＲ-MABＲVFAFig．2VariationofglucoseandVFAconcentrationinPBＲ-MABＲsystemsduringhydrogenproduction3a～3dVFAPBＲ-MABＲ。、。PBＲ、0～9h。、1～3。9～12h4～5。19。MABＲ2b～2d、。PBＲ、PBＲ-MABＲ、PBＲ-2MABＲsPBＲ-3MABＲs1647±120mg·L－110.5h、1575.2±69mg·L－19h、1283±59mg·L－19h764±27mg·L－17.5h。MABＲVFA。6161-C6H12O6+2H2O→2CH3COOH+2CO2+4H24C6H12O6+3H2O→CH3CHOHCOOH+3CO2+6H254C6H12O6+6H2O→3CH3CH2COOH+12CO2+3H26CHOHCOOH+3H2O→3CO2+6H27CH3COOH+2H2O→2CO2+4H282.3MABＲspH3PBＲ、PBＲ-MABＲ、PBＲ-2MABＲsPBＲ-3MABＲspHFig．3pHvariationduringthehydrogenproductionofPBＲPBＲ-MABＲPBＲ-2MABＲsandPBＲ-3MABＲsPBＲ。pH20。MABＲspHpH3。30～8hVFAPBＲpH7.0。8.5hPBＲpH5.08。PBＲ-MABＲ、PBＲ-2MABＲsPBＲ-3MABＲs9～12hpH。PBＲ-MABＲ、PBＲ-2MABＲsPBＲ-3MABＲspH5.54、5.685.96。VFA6～717。3MABＲMABＲVFApH。MABＲPBＲ。CH3COO－+H幑幐+CH3COOH9CH3COOH+O2→2CO2+H2O104PBＲ、PBＲ-MABＲ、PBＲ-2MABＲsPBＲ-3MABＲsFig．4ComparisonofthehydrogenproductionofPBＲPBＲ-MABＲPBＲ-2MABＲsandPBＲ-3MABＲs2PBＲTable2ComparisonofPBＲhydrogenproductioninvarioussystem/mol·mol－1/mmol·L·h－1/%PBＲ0.150.4994.4PBＲ-MABＲ0.180.6195.4PBＲ-2MABＲs0.230.7697.3PBＲ-3MABＲs0.250.8598.7glucose。2.4PBＲMABＲPBＲ、、4、2。3PBＲ1.6mmol。PBＲMABＲ。3MABＲPBＲ-3MABＲs2.971611mmol。PBＲ-2MABＲsPBＲ-3MABＲsPBＲ55.2%75.0%。2PBＲ0.49mmol·L·h－1PBＲ-MABＲ、PBＲ-2MABＲsPBＲ-3MABＲs0.62、0.760.85mmol·L·h－1。PBＲ0.15mol·mol－1glucosePBＲ-MABＲ、PBＲ-2MABＲsPBＲ-3MABＲs0.18、0.230.25mol·mol－1glucose。。MABＲ。MABＲVFApHPBＲ。12h2VFApHPBＲVFAMABＲH+。VFA。2。MABＲPBＲ-MABＲMABＲPBＲ-2MABＲs、PBＲ-3MABＲsMABＲ。3CQK01PBＲMABＲPBＲ-MABＲ、1MABＲpHPBＲ。2MABＲ。3MABＲ。4PBＲ-3MABＲs10g·L－12.8mmolPBＲ75.0%。1．ⅠJ．20062591001-10062DASDVEZIＲOˇGLUTN．HydrogenproductionbybiologicalprocessesAsurveyofliteratureJ．InternationalJournalofHydrogenEnergy200126113-183NANDIＲSENGUPTAS．MicrobialproductionofhydrogenAnoverviewJ．CriticalＲeviewsinMicrobiology199824161-844．J．201434543-465．J．201029Z179-836NIMengLEUNGDYCLEUNGMKHetal．AnoverviewofhydrogenproductionfrombiomassJ．FuelProcessingTechnology2006875461-4727．J．2008146871-8768．J．2007271151-569WANGYongzhongLIAOQiangZHUXunetal．CharacteristicsofhydrogenproductionandsubstrateconsumptionofＲho-dopseudomonaspalustrisCQK01inanimmobilized-cellphotobioreactorJ．BioresourceTechnology2010101114034-404110ZHANGChuanZHUXunLIAOQiangetal．Performanceofagroove-typephotobioreactorforhydrogenproductionbyim-mobilizedphotosyntheticbacteriaJ．InternationalJournalofHydrogenEnergy201035115284-52928161-11CHENCYYEHKLLOYCetal．Engineeringstrategiesfortheenhancedphoto-H2productionusingeffluentsofdarkfermentationprocessesassubstrateJ．InternationalJournal