光合细菌PSB应用的研究进展

:(:06C258):(1978-),,E-mai:[e-mail]::2007-10-2824120081Vol.24,No.1Jan.2008:周圆(PSB)Theprogressinapplicationresearchonphotosyntheticbacteria李福枝LIFu-zhi刘飞LIUFei曾晓希ZENGXiao-xi李小龙LIXiao-long张凤琴ZHANGFeng-qin(,412008)(TheGreenPackingandBiologyNanometerTechnologyApplicationLaboratory,HunanUniversityofTechnology,Zhuzhou,Hunan412008,China)::阐述光合细菌的应用研究现状及前景;:从光合细菌的分类结构形态菌体营养组成,有机废水处理光合产氢生物制药,提取类胡萝卜素辅酶Q单细胞蛋白和水产禽蓄养殖等方面进行阐述;:光合细菌可用来处理高浓度有机废水,可用作养殖业饲料,其菌体含有丰富的类胡萝卜素辅酶Q;:目前对光合细菌的研究主要集中于废水处理光合产氢水产和禽蓄养殖等方面的研究,在类胡萝卜素提取辅酶Q提取单细胞蛋白提取等方面研究的比较少,其应用前景十分广阔:光合细菌;废水处理;类胡萝卜素;光合产氢;单细胞蛋白;水产养殖Abstract:Objective:Toexpatiatecurrentsituationandprospectinap-pliedresearchaboutphotosyntheticbacteria;Methods:Elevenaspectssuchasclassification,morphologicalstructure,triphiccomponent,organ-icwastewatertreatment,hydrogenphotoproduction,medicamentbiologicproduction,carotenoidsextraction,coenzymeQextraction,singlecellprotein(SCP)production,fisherycultureandlivestockcultureweremainlydiscussed;Results:Photosyntheticbacteriacouldbeusedtodealwastewater,producecarotenoidsandcoenzymeQ,feedfishandlive-stock;Conclusion:Currentresearchwasmainlyonwastewatertreatment,hydrogenphotoproduction,fisherycultureandlivestockculture;muchlessincarotenoidsextraction,coenzymeQextraction,singlecellprotein(SCP)production;lohiletreirapplicationprospectwasverygood.Keywords:Photosyntheticbacteria;Treatmentoforganicwastewater;Ca-rotenoids;Hydrogenphotoproduction;Singlecellprotein;Fisheryculture(PhotosyntheticBacteria,PSB),,,,,,,,,,,,1光合细菌的定义和分类(Photosyntheticbacteria,PSB),,,(oxygenicphotosyntheticbacteria)(non-oxygenicphotosyntheticbacteria)2;,(Chromatiaceae)(Ectothiorhodospiraceae)(Purplenonsulfurbacteria)(greensulfurbacteria)(multicellularfilamentousgreenbacteria)(Helicobacteraceae)7[1]Ehrenberg1836,2H2S1883En-gellmanVanNile1931,2060,[2~4]2光合细菌的形态结构,:152;,,0.5~5m,,,,a,,715~1050nm450~550nm,CO2O2,O2,H2H2SCO2,,[5]3光合细菌菌体的营养成分(12)图1菌体成分含量Figure1Bacteriacontent图2菌体维生素含量Figure2Vitamincontentinbateria1,PSB65.45%,7.18%,2.78%,20.31%,4.28%PSB,AspThrSerGluGlyAlaValMetIleLuePheLysHisArgProTyr2.5%~12.5%[6]2,PSBB,VB1221g/g,200[7]PSBQ10,Q10Q10139482PSB(Bch1)(Carotenoids),abcde5,;80,PSB,[8]-,4光合细菌的应用4.1,:,;;[9]:,,,[10],,COD92%[11]:[12]:(COD)52840mg/L12h,92.7%;COD3860mg/L72h,99.5%[13],,,87.68%,70.95%71.90%[14],2~3d,60,;60~120,,,,,,[15],,,,,,,,,,[16]1532008年第1期(PNSB-SBR),(CODcr)5000mg/L,48h,30,CODcr500~1000mg/L,70%~90%COD0.4kg(VSS),30%~50%,COD0.2~0.4kg(SCP)[17],,(SCP),[18],:,[19],:;,,;(BOD)200mg/L,,[20]4.2,,,,,,[21]:,,ATP,NAD(P)H2H,,,HNH3H2,,NAD(P)HH[22,23]1949GestKamen[24],65mL/hL(),30mL/hL()[25],,,[26],120mmol/L,140mmol/L,,,[27](CCPR),,,(312),COD5000~5500mg/L,633.1mL/Ld,722.6mL/Ld,61.7%[28],:,;;,;;Ooshima[29],R.capsulatus,60mmol/L7mol/L,68%,25%Ken[30]R.rubrum,5mmol/L7mmol/L,52%82%[31,32],Ogbonna[33],,,Miyake[34]Koku[35]/,Turkarslan[36],[37],4.3:;,[38]Fe2+()Fe2+()2:[39]210,,:2,36.1%42.7%[40]-2:,154241:(PSB)[41],,,:,,EMEM(EffectiveMicroorganisms),51080EM90,,,B369(:369),(PSB),5,(LPO),(SOD),,,,[42]PSB,10(),60,:,(LPO),,,[43]4.4Q,,,,,,,[44]170mg/L[45]295g[46~49]YoshidaH[50]RhodobactersphaeroidesKY-4113-102002,[51~53]Q(SCP),,[54]SCP,SCP[55]4.5,B,B12,Q()1/20,[56],,,[57]:5%~28%,15%~30%,20%~23%[58],pH,,COD,,,[59](45)1~3mLPSB/100g,,,1,.()[M].:,2001,9~43.DONGXiuzhu,CAIMiaoying.Systemicidentificationhandbookforcommonbacterias[M].Beijing:SciencePress,2001,9~43.2,,.[J].,2004,6(2):35~38.WUXianhua,YangQiyin,LIUWuxing.ApplicationandResearchProgressofPhotosyntheticBacteria[J].ReviewofChinaAgriculturalScienceandTechnology,2004,6(2):35~38.3ClausD,Berkeley,RCW:in:BergeysManualofSystematicBac-teriology[J].WilliamsandWilkinsCo.,Baltimore.LondonLosAn-geis,Sydney,1984(2):1105~1139.4Imhoffj,fPetrir,SulingJ.Reclassificationofspeciesofthespira-lshapedphototrophicpurpleonsulfurbacteriaofthe-Proteinbacteria[J].InternationalJournalofSystematicBacteriology,1998(48):793~798.5,.[J].,2000,20(1):68~73.DingYanwen,AIHong.Applicationofmicrobiologyinaquiculture[J].JournalofZhanjiangOceanUnivers.i,2000,20(1):68~73.6,,.[J].,2002,21(1):91~94.LongSis,iXieShutao,DuanShunshan.Photosyntheticbacteriaandtheirappliedactualities[J].EcologicScience,2002,21(1):91~94.7.[J].1552008年第1期,1999,11(1):20~25.CuiZhanl.iThefundamentalfeaturesandthevalueofapplicationsofphotosyntheticbacteria(PSB)[J].JournalofHeilongjinagAugustFirstLandReclamationUniversity,1999,11(1):20~25.8YuJA,YeYJ,LinZX,eta.lEffectofcarotenoidsinphoto-syn-theticbacteriaoninhibitinglipidper-oxidation[J].ShanghaiTrafficUniv.,1998,32(3):107~109.9,,.[J].,2003,23(10):9~12.LuHong,ZhouJit,iWangJing.Reviewoforganiccontaminantdegra-dationbyphotosyntheticbacteria[J].IndustrialWaterTreatment,2003,23(10):9~12.10,,.[J].,2000,20(3):229~232.GuoYanghao,WangDeqiang,QiuHongduan.TreatmentofwasteaterfromglutamateindustrybyimmobilizedRhodospirillumrotatingbio-discreactor[J].ChinaEnvironmentalScience,2000,20(3):229~232.11,,.[J].,1991,25(5):25~30.YuJian,GeShiying,DingYoutu.ResyearchoncitricacidwastewaterusingPhotosyntheticbacyeria[J].JournalofShanghaiJiaotongUniversity,1991,25(5):25~30.12,.[J].,1998,3