20136446doi10.6041/j.issn.1000-1298.2013.06.027*450002。TK6A1000-1298201306-0156-06ResearchProgressofHydrogenProductionTechnologywithPhotosyntheticBacteriaZhangQuanguoWangYiKeyLaboratoryofNewMaterialsandFacilitiesforRuralRenewableEnergyMinistryofAgricultureHenanAgriculturalUniversityZhengzhou450002ChinaAbstractThecurrentresearchprogressofbiologicalhydrogenproductiontechnologywithphotosyntheticbacteriawaspresented.Themainfactorsthatinfluencedthedevelopmentofhydrogenproductiontechnologywithphotosyntheticbacterialwerediscussed.Theimprovinghydrogenproductionefficiencywithbiologicalandengineeringtechnologywereanalyzed.Theobstaclesandproblemsofthebiologicalhydrogenproductiontechnologywithphotosyntheticbacteriawereindicated.Thedevelopmenttrendsofhydrogenproductionwithphotosyntheticbacteriawerealsopreviewed.KeywordsPhotosyntheticbacterialBiohydrogenproductionHydrogenproductiontechnologyPhoto-bioreactorResearchprogress2013-01-162013-01-21*509760298632012AA05051502E-mailzquanguo@163.com、。、、。、、、。、、1。、2~3。。3。。1。、。1.1。4。200%10%~100%200%5。。6~7。1.2。。、。Turkarslan8Fascetti910Singh、311。Kim。5~7d12。13。14。1.3、15。6000lx8000lx500lx16。。。MiyakeRsphaeroides870317。1.4pHpH。pH。pH4~5pH6~8pH718。38℃25~34℃34℃19。1.57516。NH+4NH+4。PostgateN2NH3H220~21。LuddenNH+422。ScolnikNH+4NH+4nifHDK23。NH+4NH+4。Tadashi24。。NH+4ATPNH+4ATP25。22.1。。、26。Singh34Rhodopseudomonassp.BHU1~BHU4BHU1BHU41127。、、、145、、、828。Eui-JinB850-800R.sphaeroides2。2.2。KernR.rubrum50mmol/L7mmol/L30%29。YoshinohuplΔhupL330。MasukawaAnabaenahoxL-4~731。variousR.capsulatus32。。2.3ATP。。KondoR.sphaeroidesRv350~1000nm50%33Melis34。8512013。2.4。。35。36。OashimaRb.capsulataATCC2139937。ChiMeiLee38。。。。339。、、、、。1950Cook1Torzillo7.95%40。Bernado41。ChiMeiLee42KaushikNath。。12。、、、。。4。4.1、、、。、、。。95164.2、。nif。。4.3、。。1RonevichJASpeerJGKraussGetal.ImprovementofthehydrogenmicroprinttechniqueonAHSSsteelsJ.MetallographyMicrostructureandAnalysis20121279~84.2WangCCChangCWChuCPetal.ProducinghydrogenfromwastewatersludgebyClostridiumbifermentansJ.JournalofBiotechnology2003102183~92.3RonevichJADeCoomanBCSpeerJGetal.HydrogeneffectsinprestrainedtransformationinducedplasticitysteelJ.MetallurgicalandMaterialsTransactionsA20124372293~2301.4.J.200323917~22.YangSupingQuYinbo.HydrogenproductionusingphotosyntheticbacteriaJ.ModernChemicalIndustry200323917~22.inChinese5.J.20062210182~185.ZhangQuanguoWangSulanYouXifeng.EffectsoftheinfluencingfactorsofphotosyntheticbacteriagrouponhydrogenproductionJ.TransactionsoftheCSAE20062210182~185.inChinese6.J.200222544~60.JiHongfangChenXishiWangAijie.AdvanceinphotosyntheticproductionofhydrogenbyphotosyntheticbacteriaJ.JournalofMicrobiology200222544~60.inChinese7.RhodopseudomonasJ.199782194~198.ZhuHeguangZhaoQilinShiJialiang.StudyoninfluencefactorsofhydrogenproductionbyRhodopseudomonasJ.ChineseJournalofAppliedEcology199782194~198.inChinese8TurkarslanSYigitDOAslanKetal.PhotobiologicalhydrogenproductionbyRhodobactersphaeroidesO.U.001byutilizationofwastewaterfrommilkindustryM∥ZaborskyOR.BiohydrogenLondonPlentmPress1998151~156.9FascettiEDAddarioETodiniOetal.PhotosynthetichydrogenevolutionwithvolatileorganicacidsderivedfromthefermentationofsourceselectedmunicipalsolidwastesJ.InternationalJournalofHydrogenEnergy1998239753~760.10.J.2006402177~180.ZhangJunheZhangQuanguoShiYuzhongetal.StudyonhydrogenproductionbyhigheffecthydrogenproductionPSBgroupwithpigdejectaassubstrateJ.JournalofHenanAgriculturalUniversity2006402177~180.inChinese11SinghSPSrivastavaSCPandeyKD.HydrogenproductionbyRhodopseudomonasattheexpenseofvegetablestarchsugarcanejuiceandwheyJ.InternationalJournalofHydrogenEnergy1994195437~440.12.5m3D.2008.13.J.2005262248~252.ZhangQuanguoLeiTingzhouYouXifengetal.StudyonhydrogenproductioninfluencefactorJ.ActaEnergialSolarisSinica2005262248~252.inChinese14.J.2011393340~343.YueJianzhiLiGangZhangQuanguoetal.OverviewonpretreatmenttechnologyaboutpromotingtheenzymatichydrolysisoflignocellulosicwithbiomassJ.JiangsuAgriculturalSciences2011393340~343.inChinese15.J.200323917~22.YangSupingQuYinbo.HydrogenproductionusingphotosyntheticbacteriaJ.ModernChemicalIndustry200323917~22.inChinese061201316.J.200728101135~1139.ZhangQuanguoShiYuzhongZhangJunheetal.InfluenceofsolarspectrumoncharactersiticsofgrowthandhydrogenproductionofphotosyntheticbacteriaJ.ActaEnergiaeSolarisSinica200728101135~1139.inChinese17MiyakeJKawamuraS.EfficiencyoflightenergyconversiontohydrogenbythephotosyntheticbacteriumRhodobactersphaeroidesJ.InternationalJournalofHydrogenEnergy1987123147~149.18.J.199535165~73.SunQiXuXiangyangJiaoYangwen.ThestudyonhydrogenproductionconditionswithphotosyntheticbacteriaJ.ActaMicrobiologicaSinica199535165~73.inChinese19.J.200728111253~1255.20PostgateJR.ThefundamentalsofnitrogenfixationM.CambridgeCambridgeUniversityPress1982.21.J.2000106481~490.22MaddenMSLuddenPWPaustianTDetal.EffectsofhomocitratehomocitratelactoneandfluorohomocitrateonnitrogenaseinNifV-mutantsofAzotobactervinelandiiJ.JournalofBacteriology1991173175403~5405.inChinese23ScolnikPAViroscoJHaselkornR.Thewild-typegeneforglutaminesynthetaserestoresammoniacon