广州典型排放源废水中抗生素的污染特征和去除效果

JournalofSouthChinaNormalUniversityNaturalScienceEdition201850111－20doi10．6054/j．jscnun．20180102017－11－28《》http//journal．scnu．edu．cn/nU1701242“”*[e-mail]@gig．ac．cn．15222314144*14*1．∥5106402．5100303．5101804．5106315．100049－－SPE－ＲＲLC－MS/MS5、、、41．24、14、15、121350．917μg/L、127μg/L、11．8μg/L3．61μg/L664μg/L．99．7%99．5%、49．3%、63．0%26．9%．51．21kg/d89．3μg/·d．．X703A1000－5463201801－0011－10OccurrenceandＲemovalofAntibioticsfromWastewaterofTypicalEmissionSourcesinGuangzhouChinaWEIXiaodong15LIUYexin2ZHOUZhihong2OUHui2ZHANGJingwen3ZHAOJianliang14LIUYousheng14CHENJun4*YINGGuangguo14*1．StateKeyLaboratoryofOrganicGeochemistry∥GuangzhouInstituteofGeochemistryChineseAcademyofSciencesGuangzhou510640China2．GuangzhouEnvironmentalMonitoringCenterGuangzhou510030China3．GuangzhouEnvironmentalTechnologyAssessmentCenterGuangzhou510180China4．TheEnvironmentalＲesearchInstituteSouthChinaNormalUniversityGuangzhou510631China5．UniversityofChineseAcademyofSciencesBeijing100049ChinaAbstractSurveysconcerningtheoccurrenceandremovalcharacteristicsof41targetantibioticsfrom5classesoftypicalemissionsourcesmunicipalwastewatertreatmentplantWWTPpharmaceuticalfactoryhospitalland-fillandlivestockfarmindowntownGuangzhouwereconducted．AntibioticswereidentifiedandquantifiedusingrapidresolutionliquidchromatographytandemmassspectrometryＲＲLC－MS/MScombinedwithsolid－phaseex-tractionSPEforpretreatment．Theresultsshowedthat24141512and13antibioticsweredetectedinWWTPspharmaceuticalfactorieshospitalslandfillsandlivestockfarmsrespectivelyandthehighestdetectedconcentrationofsingleantibioticwas0．917μg/LoxytetracyclineinWWTPs127μg/Lroxithromycininphar-maceuticalfactories11．8μg/Lroxithromycininhospitals3．61μg/Lanhydro－erythromycininlandfillsand664μg/Loxytetracyclineinlivestockfarms．Antibioticswerewellremovedinlandfillsandlivestockfarmswiththetotalremovalrateof99．7%and99．5%andlimitedremovedinWWTPspharmaceuticalfactoriesandhos-pitalswiththetotalremovalrateof49．3%63．0%and26．9%respectively．Themassloadandemissionpercapitaoftotalantibioticsdischargedintotheenvironmentinaquaticphasewas1．21kg/dand89．3μg/inhabitant·dindowntownGuangzhou．Incompleteremovedantibioticsfromtypicalemissionsourcesconstituteoneofsignificantsourcesofantibioticcontaminationintheaquaticenvironment．Keywordsantibioticemissionsourcecontaminationcharacteristicremovalefficiency．1．234．2．、、、5．6－8．3692700t5．．．11．1411．、Sigma－AldrichDr．EhrenstorferGmbH．4mol/LH2SO4pH34h．91－D4、－13C－D3、－D4、－D8、－D3－D3TorontoＲesearchChemicals－D4Dr．EhrenstorferGmbH－13C6CambridgeIsotopeLaboratoriesSigma－Aldrich．MerckLC－MSTediaSigma－AldrichOasisHLB6mL500mg200mgWatersGF/F0．7μmWhatman450℃4h．Na4EDTA、NaOHMilliporeMilli－Q．1．2100mg/L．NaOH1mol/L0．5∶99．5－20℃．1．32015125、、、．2．2．31L．50mL400μL4mol/LpH3．4℃48h．1．41L0．5gNa4EDTA100μL1mg/L．．10mL10mLHLB5～10mL/min．25mL5%/2．10mLNa4EDTA．120min．10mL1mL－20℃．100μL0．2%2mmol/L1∶1100μL．21501Table1InformationoftargetantibioticsandtheirinternalstandardsCASSulfadoxineSDOSulfonamides2447－57－6C12H14N4O4SSulfamethoxazole－D4SulfadimethoxineSDMSulfonamides122－11－2C12H14N4O4SSulfamethoxazole－D4SulfaquinoxalineSQXSulfonamides59－40－5C14H12N4O2SSulfamethoxazole－D4SulfachlorpyridazineSCPSulfonamides80－32－0C10H9ClN4O2SSulfamethoxazole－D4SulfameterSMSulfonamides651－06－9C11H12N4O3SSulfamethazine－13C6SulfamonomethoxineSMMSulfonamides1220－83－3C11H12N4O3SSulfamethoxazole－D4SulfamethazineSMZSulfonamides57－68－1C12H14N4O2SSulfamethazine－13C6SulfisoxazoleSXSulfonamides127－69－5C11H13N3O3SSulfamethoxazole－D4SulfamerazineSMＲSulfonamides127－79－7C11H12N4O2SSulfamerazine－D4SulfathiazoleSTZSulfonamides72－14－0C9H9N3O2S2Sulfamethazine－13C6SulfamethoxazoleSMXSulfonamides723－46－6C10H11N3O3SSulfamethoxazole－D4SulfadiazineSDZSulfonamides68－35－9C10H10N4O2SSulfamethazine－13C6SulfapyridineSPDSulfonamides144－83－2C11H11N3O2SSulfamethazine－13C6SulfacetamideSCTSulfonamides144－80－9C8H10N2O3SSulfamethazine－13C6DifloxacinDIFFluoroquinolones98106－17－3C21H19F2N3O3Ciprofloxacin－D8SarafloxacinSAＲFluoroquinolones98105－99－8C20H17F2N3O3Ciprofloxacin－D8FleroxacinFLFluoroquinolones79660－72－3C17H18F3N3O3Ciprofloxacin－D8MarbofloxacinMAＲFluoroquinolones115550－35－1C17H19FN4O4Ciprofloxacin－D8OfloxacinOFXFluoroquinolones82419－36－1C18H20FN3O4Ciprofloxacin－D8EnrofloxacinEFXFluoroquinolones93106－60－6C19H22FN3O3Ciprofloxacin－D8DanofloxacinDANFluoroquinolones112398－08－0C19H20FN3O3Ciprofloxacin－D8LomefloxacinLFXFluoroquinolones98079－51－7C17H19F2N3O3Ciprofloxacin－D8PefloxacinPEFFluoroquinolones70458－92－3C17H20FN3O3Ciprofloxacin－D8CiprofloxacinCFXFluoroquinolones85721－33－1C17H18FN3O3Ciprofloxacin－D8NorfloxacinNFXFluoroquinolones70458－96－7C16H18FN3O3Ciprofloxacin－D8CarbadoxCAＲFluoroquinolones6804－7－5C11H10N4O4Thiabendazole－D4ChlortetracyclineCTCTetracyclines57－62－5C22H23ClN2O8MeclocyclineOxytetracyclineOTCTetracyclines79－57－2C22H24N2O8Thiabendazole－D4DoxycyclineDCTetracyclines564－25－0C22H24N2O8MeclocyclineTetracyclineTCTetracyclines60－54－8C22H24N2O8Thiabendazole－D4MethacyclineMTTetracyclines914－00－1C22H22N2O8MeclocyclineTylosinTYLMacrolides1401－69－0C46H77NO17