过氧化氢酶的研究与应用新进展刘灵芝

2009,Vol.26No.3　Chemistry&Bioengineering15　　　:国家科技支撑计划资助项目(2008BAD91B01),北京市科技新星计划资助项目(2006B22):2008-11-13:刘灵芝(1983-),女,河南人,硕士研究生,研究方向:生物化工;通讯联系人:罗晖,副教授。E-mail:[e-mail]。1,2,1,　1,2,2,2,　1(1.北京科技大学生物科学与技术系,北京100083;2.北京科技大学环境工程系,北京100083)　　　:过氧化氢酶是一种广泛存在于好氧微生物和动、植物体内、催化效率较高且具有重要工业应用价值的酶。在论述过氧化氢酶的类型划分、结构特点和功能的基础上,阐述了过氧化氢酶的分离纯化和极端环境下微生物产过氧化氢酶的研究进展,并对过氧化氢酶在食品、环保、造纸、纺织等行业中的应用进行了介绍。:过氧化氢酶;极端微生物;应用;进展:Q814　　　　　:A　　　　　:1672-5425(2009)03-0015-04　　(Hydrogenperoxidase)(Catalase,CAT),、,,。,、、、、[1,2]。1　CAT,CAT,、、CAT。CATCATCAT,CAT,CAT。1989,Goldberg[3]CAT[4],CAT(MonofunctionalcatalaseTypicalcatalase)、CAT(Catalase-peroxidase,CAT-POD)CAT(Mn-catalasee)。CAT:(1)CAT,;(2)CAT,(MnCAT)[5]。2　CATCAT,。,CAT,4,,,4,200～340kDa[6]。CATFe3+,2-。CAT,、。,,CAT,CAT1[7],,。CAT,、pH、H2O2。,3CAT,Kono[8]Barynin[9]。(ESR)2,Mn2(Ⅱ,Ⅲ)Mn2(Ⅲ,Ⅳ)。Mn2(Ⅱ,Ⅲ),Mn2(Ⅲ,Ⅳ)。3　CAT3.1　CAT1937Sumner,[10～13]。1999,,:/2009316　　　,[14]。[15]、DEAE-SepharoseFF,。[16],SephadexG-100PEG6000。,6×His-tag,[17,18]。Gustavo[19]6×His-6×ArgC(HRPC),130,98.5%。3.2　CATCAT,。()、,CAT。CAT、、,H2O2,。3.2.1　CAT1973,Kurono[20]BacillusNo.KU-1,,CAT,。1990,Yumoto[21]BacillusYN-2000CAT。1995,Hicks[22]BacillusfirmusOF4,3CAT。2001,Gudelj[23]Bacillussp.SF,CAT,。2002,Phucharoen[24]CAT。3.2.2　CAT1988,Suvit[25]Bacillusstearothermophi-lusIAM11001CAT,70℃,30℃。1999,Kagawa[26]CAT,,。[27]WSH03201,CAT,,。3.2.3　CAT,,,CAT[28]。,,PCRperA,。,SDS-PAGECAT20%,,484.56U·mg-1。,、,[29]。3.3　CATCAT,、、、,,CAT。1987,,CAT,[30]:(1);(2);(3);(4);(5);(6)。,。Martell[31]H2O2,,H2O2,Mn(Ⅱ)H2O2,。3,,,。CAT,,4,CAT。4　CAT4.1　:/2009317　　　CAT。CATH2O2O2,CAT[27]。CAT。,CAT。,[32]。4.2　10%～15%[28]。CATH2O2,[33]。(HRP),[34]。4.3　H2O2,SO2H2O2。,H2O2,,CAT10minH2O2,[33]。2004(INEEL)Thermusbrockianus(),,R&D2004100[35]。4.4　,,、(3);,。,,,。、、、,[36,37]。4.5　H2O2,H2O2、,,H2O2[38,39]。,,H2O2,,,[40]。5　1937CAT,。,。,,CAT,,CAT。,,CAT。:[1]　SchraderM,FahimiHD.Peroxisomesandoxidativestress[J].BiochimicaetBiophysicaActa(BBA),2006,1763(12):1755-1766.[2]　ZamockyM,FurtmǜllerPG,ObinC.Evolutionofcatalasesfrombacteriatohumans[J].Antioxidants&RedoxSignaling,2008,10(9):1527-1548.[3]　GoldbergI,Hochrnan,A.ThreedifferenttypesofcatalaseinKlebsiellapneumoniae[J].ArchBiochemBiophys,1989,268(1):124-128.[4]　ZámockyM,KollerF.Understandingthestructureandfunctionofcatalase:Cluesfrommolecularevolutionandinvitromutagene-sis[J].ProgressinBiophysicsandMolecularBiology,1999,72(1):19-66.[5]　,.[J].(),2006,5(6):465-468.[6]　JacekS,LoewenPC.Diversityofpropertiesamongcatalases[J].ArchiversofBiochemistryandBiophysics,2002,401(2):145-154.[7]　DismukesGC.Manganeseenzymeswithbinuclearactivesites[J].ChemRev,1996,96(7):2909-2926.[8]　KonoY,FridovichI.Isolationandcharacterizationofthepseudo-catalaseofLactobacillusplantarum———Anewmanganesecontai-ningenzyme[J].JBiolChem,1983,258(10):6015-6019.[9]　BaryninVV,GrebenkoAI.T-catalaseisanonhemecatalaseofextremelythermophilicbacteriumThermusthermophilus[J].DoklAkadNaukSSSR,1986,286(2):461-464.[10]　ReubsaetFA,VeerkampJH,BrǜckwilderML,etal.Peroxiso-maloxidasesandcatalaseinliverandkidneyhomogenatesofnor-malanddi(ethylhexyl)phthalate-fedrats[J].IntJBiochem,1991,23(9):961-967.[11]　NakamuraK,WatanabeM,SasakiY,etal.Purificationandchar-acterizationoflivercatalaseinacatalasemicbeagledog:Compari-sonwithnormaldoglivercatalase[J].TheInternationalJournalofBiochemistryandCellBiology,2000,2(1):89-98.[12]　YumotoI,IchihashiD,IwataH,etal.Purificationandcharac-terizationofacatalasefromthefacultativelypsychrophilicbacte-riumVibriorumoiensisS-1Texhibitinghighcatalaseactivity[J].:/2009318　　　JBacteriol,2000,182(7):1903-1909.[13]　KangYS,LeeDH,YoonBJ,etal.Purificationandcharac-terizationofacatalasefromphotosyntheticbacteriumRhodospi-rillumrubrumS1grownunderanaerobicconditions[J].JournalofMicrobiology,2006,44(2):185-191.[14]　GoncalvesVM,CezardeCerqueiraLeiteL,RawI,etal.Puri-ficationofcatalasefromhumanplacenta[J].BiotechnologyandAppliedBiochemistry,1999,29(1):73-77[15]　,.[J].,1999,11(4):25-29.[16]　.[J].,2006,27(12):311-314.[17]　VitalyGrigorenko,TatianaChubar,YuryKapeliuch,etal.NewapproachesforfunctionalexpressionofrecombinanthorseradishperoxidaseCinEscherichiacoli[J].BiocatalysisandBiotransfor-mation,1999,17(5):359-379.[18]　RyanBJ,FágáinC.Arginine-to-lysinesubstitutionsinfluencerecombinanthorseradishperoxidasestabilityandimmobilisationeffectiveness[J].BMCBiotechnology,2007,7(1):86-94.[19]　GustavoLevin,FernandoMendive,HéctorMTargovnik,etal.Geneticallyengineeredhorseradishperoxidaseforfacilitatedpur-ificationfrombaculovirusculturesbycation-exchangechroma-tography[J].JBiotechnol,2005,118(4):363-369.[20]　KuronoY,HorikoshiK.AlkalinecatalaseproducedbyBacillusNo.KU-1[J].AgrBiolChem,1973,37(11):2565-2570.[21]　YumotoI,FukumoriY,YamanakaT.Purificationandcharac-terizationofcatalasefromafacultativealkalophilicBacillus[J].JBiochem,1990,108(4):583-587.[22]　HicksDB.Purificationofthreecatalaseisozymesfromfaculta-tivelyalkaliphilicBacillusfirmusOF4[J].BiochimBiophysAc-ta,1995,1299(3):347-355.[23]　GudeljM,FruhwirthGO,PaarA,etal.Acatalase-peroxidasefromanewlyisolatedthermoalkaliphilicBacillussp.withpoten-tialforthetreatmentoftextilebleaching