海洋真菌多不饱和脂肪酸的快速检测

XXX(430074):EPADHA,:CHCl32KOH2CH3OH,;KOH2CH3OH,,EPADHA7132%7158%:,EPA,DHA,GC:O657.71:A:025322654(2001)012073204AFASTDETERMINATIONOFPOLYUNSATURATEDFATTYACIDSPRODUCEDBYMARINEFUNGUS3BIANShu2GuangZHANGXiao2YuZHANGLei33HUANGKai2Xun(Dept.ofChemistry,HuazhongUniversityofSei.&Tech.Wuhan430074)Abstract:AfungusthatiscapableofproducingEPAandDHAwasisolatedfromseawater.Afastextrac2tionandmethylesterificationofmicrobiallipidwereestablishedaswellastheircontentdeterminationbycapillarygaschromatographyusingexternalcalibrationmethod.TheresultsshowedthatthemethylesterprepareddirectlyformthewetmyceliatreatedwithCHCl32KOH2CH3OHislesstime2consumingandthemethodfitsforthefastdetermination.TodetermineitsEPAandDHAcontent,thewetmyceliawashy2drolyzedaccordingtoWeibulsmethodandthelipidobtainedwasmethylesterifiedwithKOH2MeOH,theresultsobtainedbycapillarygaschromatographyusingexternalcalibrationmethodwere7.32%and7.58%respectively.Keywords:Polyunsaturatedfattyacids,Eicosapentaenoicacid,Docosahexaenoicacid,Gaschromatogra2phy(PUFA),1622,C2(GLA),(EPA)(DHA)[1],GLAEPA;DHA,[2]9,,,37200128(1)XXX:1999211217,:2000201205(No.996001019G)PUFAs,,,,PUFA[3,4],,,EPADHA,HP2689011.12860d1.21.2.1PUFA:GLA,EPADHASigma,:11212:HP26890;FID;HP3398AGCChemstation;HP2IN2NOWAX(CrosslinkedPolyethyleneGlycol):30m0.25mm0.25LmHQL1502B:113Ml:NaCI30g,KCl1g,KH2PO41g,(NH4)2SO40.2g,NaHCO30.1g,25g,2g,20g,H2O1000mLM2:10g,10g,10g,5g,20g,H2O1000mL5060:0.5g(100)5mL,1.0mL330mL,10%M1,M2,:20%,2%11410-1,10-2,10-3,10-4,10-5M1,M2,28,,12.540,100mL,150römin,2848h,2024h,1524h,0524h,3115PUFA11511(CHCl32KOH2CH3OH):1g,4mLCHCl32mL0.5molöLKOH2CH3OH,2min,5010min(N2),2min,3.6mL,1min,,CHCl311512:,HP26890:150=15ömin200(15min)=2ömin240(2min)260,260H2:30mLömin,:150mLömin,N2:30mLömin,:1mLömin(),:29mLömin,1001,1LL47200128(1)2211PUFA1(a),(b)GLAEPADHAM11022,1022EPADHA1(a)GLADHA1(b)212250,1020Pa,:(A)SoxhletCHCl3öCH3OH(21)24h;(B)(bp3060)24h;(C)24h;(D)[5];(E)80%N2,2ABCDE2ö(21),,,,;,;,;,,,213M1102230100mg25mL,2mL(bp3060)ö(11,vöv),2mL0.5molöLKOH2CH3OH1015min,GLA,EPADHA(LgömL)13,,(502000LgömL),0.99857200128(1)EPADHA:(7.320.25)%(7.580.30)%,(n=3)1rGLAY=-52253.15+1074.00x0.9984EPAY=14887.36+1800.28x0.996DHAY=-13980.48+1428.69x0.99873,,PUFA,N2CHCl32KOH2CH3OH,,PUFA,,Soxhlet,,,,,,ö,,BF3,,[6];2(16h)[7];222,(1015min),,HP26890,,,,,,,,,,,[1]DyerbergJ.NutritionReviews,1986,44(4):125138.[2].,1988,37(12):10811096.[3]CohenZ,HeimerY.AmericanOilChemistsSociety,1992,238273.[4]KendrickA,RatledgeC.Lipids,1992,27:1520.[5].().:,1990,2930.[6]Ke2Shun.Jaocs,1994,71(11):11791187.[7]ChristieWW.LipidAnalysisis,Oxford:PergamonPress,2nded.,1982.2001,17,102,:,:100080:[phone]:[phone]E-mail:[e-mail]67200128(1)