搜索
2002,22(6)546~550ChinaEnvironmentalScience,,,,(,210095),.,NaCl45000mg/L,1.6kgCODCr/(m3⋅d),CODCr96.6%.,,15d109cfu/(g⋅VSS)1011cfu/(g⋅VSS),.X703A10006923(2002)06054605Industrialhypersalinewastewaterbiochemicaltreatmentofsalt-tolerantsludgeacclimationanditsmechanisms.HEJian,LIShun-pengCUIZhong-li,CHENLi-wei,GUXiang-yang(DepartmentofMicrobiology,CollegeofResourceandEnvironmentalScience,NanjingAgriculturalUniversity,Nanjing210095,China).ChinaEnvironmentalScience.2002,22(6)546~550AbstractThefeasibility,characterandbiologicalprocessofindustrialhypersalinewastewaterbiochemicaltreatmentofsalt-tolerantsludgeacclimationwerestudiedthroughcomparingtheactivatedsludgeacclimationunderhighandlowsalineconditions.Theresultshowedthattakingsaltastheselectivepressure,salt-tolerantsludgewithhighdegradationactivitycouldbeacclimated.WhentheNaClconcentrationis45000mg/Landthevolumetricloadingis1.6kgCODCr/(m3⋅d),theCODCrremovalratecouldreachto96.6%.Analysisofmicrobialcommunitiesintheacclimationofsalt-tolerantsludgeshowsthattheamountofsalt-tolerantphenylaceticacid-degradingbacteriaincreasedfrom109cfu/(g⋅VSS)to1011cfu/(g⋅VSS)in15days,withincreaseofinfluentsaltconcentrationthusbecomingthedominantphysiologicalcommunityinthesludge.Keywordsindustryhypersalinewastewaterbiochemicaltreatmentsalt-tolerantsludgeacclimationsuccessionofdominantphysiologicalcommunitiessalt-tolerantmicroorganisms[16],,,,[16,11],[8,9],.,.11.1,pH2~3,NaCl(HClpHNaCl22.5%)(8600mg/L)CODCr(18500mg/L).,86.6%,2.6×109cfu/(g⋅VSS).1.21.2.1(),NaCl1%~5%,45000mg/LNaCl(5)20020311(2000.21)6547.,,,(40),CODCr,.7500mg/L(30).1.2.2SVITSSVSS[10].DOJPB-607,.CODCrCl,[10].Cl[10].258.8nm.,,0.2%[11],30min,1mL.10000mg/LNaCl;45000mg/LNaCl;5000mg/LNaCl;45000mg/LNaCl.:5g,10g,20g,NaCl,1000mL,pH7.2.:(NH4)2SO41g,K2HPO41.4g,KH2PO40.6g,MgSO40.1g,CaCl20.1g,0.3g,20g,NaCl,1000mL,pH7.5.22.1,(OUR).OUR.NaCl(1000mg/L),OURNaCl,5000mg/L,OUR28.0mgO2/(L⋅h),25000mg/L,OUR12.0mgO2/(L⋅h),45000mg/L,OUR3.2mgO2/(L⋅h),,;30,OUR0.3mgO2/(L⋅h),OUR0.5mgO2/(L⋅h),.2.2,,,(1),.NaCl45000mg/L,1.6kgCODCr/(m3⋅d),CODCr96.6%,(2).1NaClFig.1ThechronologicalcurveofNaCllevel2CODCrFig.2ThechronologicalcurveofCODCrremovalefficiencyandvolumetricloadingCODCrCODCrNaCl(×104mg/L)01234505101520253035404550d02040608010012016101826344250dCODCr%0.00.51.01.52.0[kgCOD/(m3⋅d)]548222.32.3.1,,0.02~0.1mm(3A).2d,(3B),,.10d,,,,2~6mm,(3C,3D),,.3Fig.3StructurechangeoftheinoculaA.(×)B.2d(×)C.14d(×)D.38d(×)2.3.2,4,,6d,,..,,.2.3.3,,SVI(5),SVI50%,.[2],SVI.,,,,Na+,Na+,Ca2+Mg2+.()(),.4Fig.4Chronologicalcurveofsludgelevel5SVIFig.5ThechronologicalcurveofSVI2.4,,109cfu/(g⋅VSS),,107cfu/(g⋅VSS),102cfu/(g⋅VSS),,,,.,.1,1,CDAB02040608005101520253035404550(d)SVI(mL/g)(g/L)123456705101520253035404550d6549.10d109cfu/(g⋅VSS)1011cfu/(g⋅VSS),cfu/(g⋅VSS)cfu/(g⋅VSS),,.,,,1%.1(cfu/g⋅VSS)Table1Thechronologicalchangeofmicrobialamountandphysiologicalcommunities(cfu/g⋅VSS)(d)14.3×7.1×2.1×n.d.4×××n.d.6×4.3×3.1××10××××14××××20××××26××××30××××38××××50××××:n.d.14d,,,(),.,,,1011cfu/(g⋅VSS),,,30d1.2×1011cfu/(g⋅VSS),.38d,,.,,,3,(Pseudomonassp.,P1),(Plesiomonassp.,PL1),(Pseudomonassp.,P2).,,,3,,10d.,2[(Arthrobactersp.A1),(Pseudomonassp.P3)],30d,3,.5(610nm,),2,2P1P2PL15000~10000mg/L,A1P345000~60000mg/L,A1P3P1P2PL1,,.2Table2Comparisonoftheabilitytosalt-toleranceofthedominantstrains(mg/L)500010000250004500060000P10.6230.5910.2520.0410.012P20.6850.5500.1540.0120.010PL10.5160.4980.1370.0010.031A10.0840.1310.3560.4970.512P30.1030.1540.3670.4790.491,,,,,,,.,55022.33.1,NaCl45000mg/L.3.2,.3.3,.[1].[M].1997.[2],,,.[J].,1999,15(9):1819.[3],,,.[J].,2000,21(1):106108.[4],,.[J].,2000,18(2):1216.[5]YanceyH,ClarkME,HandSC,etal.Livingwithwaterstress:evolutionofosmolytesystems[J].Science,1982,217:12141222.[6]HamodaMF,Al-atlarIMS.Effectsofhighsodiumchlorideconcentrationonactivatedsludgetreatment[J].Wat.Sci.Tech.,1995,31(9):6172.[7],,,.[J].,1999,20(3):104106.[8],.[J].,1987,19(2):2024.[9],.[J].,2000,18(1):37.[10].[M](3).:,1997.216220.[11]AnLi,GuGuowei.Thetreatmentofsalinewastewaterusingatwo-stagecontactoxidationmethod[J].Wat.Sci.Tech.,1993,28(7):3137.(1973),,,,,.4.(NRC)(EPA),EPA.1996(SDWA),EPA1998,(CCL).5010,EPA.CCL,EPA.SDWA5CCLs,EPA,NRC.10000~100000,,NRC..EnvironmentalScience&Technology,September1,342A343A(2002)