好氧段对反硝化除磷系统的影响高小平

　2005年3月重庆大学学报(自然科学版)Mar.2005　第28卷第3期JournalofChongqingUniversity(NɑturɑlScienceEdition)Vo.l28　No.3　　:1000-582X(2005)03-0106-04*高小平,吉芳英,丁彩娟,左　宁,张　斌(重庆大学三峡库区生态环境教育部重点实验室,重庆　400030)　:生物除磷系统在厌氧/缺氧交替变化的环境中可以发生反硝化除磷现象,通过研究发现:没有好氧段的A/A-SBR系统除磷能力低于有好氧段的A/AO-SBR系统,而且随着运行时间的增加A/A-SBR系统的除磷能力逐渐减弱,污泥产率也从起始的0.22gMLSS/gCODd逐渐下降趋于零增长甚至负增长。试验结果表明,设置后好氧段是保证反硝化除磷系统稳定运行的关键。但是,较长的好氧时间将导致NO3-N的积累,并抑制A/AO-SBR系统除磷,而0.5h的后好氧时间既可以确保A/AO-SBR反硝化除磷系统的稳定运行又可以获得好的除磷效果。:反硝化除磷;好氧时间;A/A-SBR;A/AO-SBR;生物除磷脱氮　　:X505:A　　,//,、NO-3-N。NO-3-N、,ATP2/3。,,。,,[1-3],,75%,50%。,2:1)/(A/A)、[4-6];2),A/A,[7-8]。,。1　试验材料与方法1.1　试验装置A/A-SBRA/AO-SBR2。SBR,0.25m、0.3m,12L。,,A/AO-SBR。SBR、、。SBR、,0.58。1.2　试验方法1.2.1　原水水质,1。1　mg/LCOD246～278NH3-N20～25PO3-4-P4.3～5.8pH7.0～7.5(CaCO3)330～3901.2.2　研究方法1)2,SBR,/,,*:2004-11-22:(50278101);(1-57):(1979-),,,,。0.5mg/L、90%,/,,////,COD、NO-3-N。、CODA/A-SBR,,NO-3-N20～30mg/L。2。A/A-SBRA/AO-SBR,SBR8h,。2SBRMLSS,2.5g/L,SRT=25d。A/ASBR、A/A/OSBR2。2　hA/O2——4.02.0A/A20.53.50.51.5A/A2—4.002.0A/AO2—4.00.51.5　　2)5SBR,、SRT=25d,3。NO-3-N30mg/L。PO3-4-P、NO-3-N。3　h1#2.03.502.52#2.03.50.52.03#2.03.51.01.54#2.03.51.51.05#2.03.52.00.51.2.3　分析方法COD:HACH-COD(DR/2010)(GB11914);DO:YSI(YSI-5100);NH3-N:;PO3-4-P:-;NO-3-N:;NO-2-N:N-(1-)-;:;《》(4)。2　试验结果及分析2.1　反硝化除磷系统的启动1。1　1A/O、A/A2,1:1)/,A/O,90%,;2)A/OA/A,,26%。A/A,40%～60%。,,。2.2　A/A-SBR和A/AO-SBR系统运行效果对比2.2.1　反硝化除磷效果对比2。2　A/A-SBRA/AO-SBR2COD=246～278mg/L、PO3-4-P=4.3～5.8mg/L、NH3-N=20～25mg/L,A/A-SBRA/AO-SBR2。2:1)A/AO-SBRPO3-4-P,1mg/L;2)A/A-SBRPO3-4-P,,。,。2.2.2　MLSS逐日变化过程及污泥产率30d,A/A-SBRA/AO-SBRMLSS3。107第28卷第3期　　　　　　　　　　高小平等:　好氧段对反硝化除磷系统的影响3　2MLSSYs3,SRT=25d,2,,。COD,(COD)。,A/AO-SBRYs=0.22gMLSS/gCODd,;A/A-SBRYs,Ys,Ys=0.044gMLSS/gCODd。MLSSYs:A/AO-SBRYsMLSS,SRT=25d,,,、SRT,MLSS;A/A-SBR,MLSSYs,A/A,。2.2.3　两系统系统N、P转化过程对比A/A-SBRA/AO-SBRN、P45。4　2d42dN、P。4,A/A-SBRA/AO-SBRN、P,,NO-3-NPO3-4-P,70%P,N95%。4,A/A-SBR,PO3-4-P,NO-3-N,NH3-N。525dA/A-SBRA/AO-SBRN、P。52:A/AO-SBRN、P,,A/A-SBR,。。5　25d2.2.4　两系统除磷能力分析A/A-SBRA/AO-SBR[9],,:A/O/A/O(2h/3h/1.5h/0.5h/1h)。32.5g/L,SRT=25d,。43、、。4　、、%MLSS/gL-1YsPA/A2.5～0.780.0441.8802.675—A/AO2.5～2.220.2203.3455.7206.39A/O/A/O2.5～3.100.2453.570—6.69　　,,(1):A=B×C×1000(1):A,mg/d;B,g/d;C,%。108重庆大学学报(自然科学版)　　　　　　　　　　　　　　　　　　　2005年3,5。5　B/g/dC/%A/mgd-1P/%A/A0.7872.67521.0519.9A/AO1.136.3972.268.1A/O/A/O1.346.6989.684.5　　:P5.05mg/L,P106mg/L。,A/A-SBR,A/AO-SBR,A/O/A/O-SBR。2.3　好氧时间对反硝化除磷系统的影响5PO3-4-P6,,5PO3-4-P。,,,P,。,,2#P,PO3-4-P,6。1#A/A-SBR,,,3#、4#、5#,NH3-N,NO-3-N10～12mg/L,NO-3-N,。2#0.5h,,NH3-N,NO-3-N5mg/L,CODNO-3-NP,A/AO-SBR。6　2mg/L1#2#3#4#5#5.305.305.305.305.3021.1722.0622.7321.9322.991.601.491.781.581.72—0.710.550.420.30/%69.8186.6089.6292.0894.343　结　论1)A/A-SBRA/AO-SBR,A/A6　5,,,A/AO-SBR。2),NO-3-N,。0.5hA/AO-SBR。3)A/A-SBR;A/AO-SBR,。:[1]　,.[J].,2003,19(8):33-35.[2]　JENPETER,KEREN-JESPERSEN,MOGENSHENZE,eta.lBi-ologicalPhosphorusReleaseandUptakeAlternationAnaero-bicandAnoxicConditionsinaFixed-filmReactor[J].WatRes,1993,27(4):617-624.[3]　,,MARKVANLOOSDRECHT.BCFS[J].,2002,28(9):7-9.[4]　,,./SBR[J].,2003,4(6):9-12.[5]　,,,.、[J].,2003,19(1):33-36.[6]　NGWJ,ONGSL,HUJY.DenitrifyingPhosphorusRe-movalbyAnaerobic/AnoxicSequencingBatchReactor[J].WatSciTech,2001,43(3):139-146.[7]　,,.[J].,2002,18(9):4-7.[8]　,,,.[J].,2003,19(3):8-10.[9]　,,,.SBR[J].,2002,18(5):1-5.(120)109第28卷第3期　　　　　　　　　　高小平等:　好氧段对反硝化除磷系统的影响InfluenceofpHValueonFermentationofL-asparaginaseandControlPANHong-chun1,2,WANGBo-chu1,LIUHong1,2,CHENZhen-wen2,ZHOULing2,LIYu-lin2(1.KeyLaboratoryforBiomechanicsandTissueEngineeringUndertheStateMinistryofEducation,CollegeofBioengineering,ChongqingUniversity,Chongqing400030,China;2.SouthwestPharmaceuticalsCompanyofLimitedLiability,TaijiGroup,Chongqing400038,China)Abstract:TheinfluenceofpHvalueonthefermentationofL-asparaginaseisextremelysignifican.tTheinfluenceofpHvalueonthesynthesisofL-asparaginaseduringfermentationwasstudiedundervariousbuffersandthechangeofpHval-ueinthetheshakedflask.ThepHvalueduringfermentationwascontrolledbyfeedingphosphoricacidautomatically.Itprolongedthefermentationperiod,effectivelypreventedtheprematureautolysisofcellsandsignificantlyacceleratedthesynthesisofL-asparaginase.TheyieldofL-asparaginase(110u/mL)increasedby83%comparedwiththatbybatchfermentation.FermentationkineticsindicatethatthesynthesisofL-asparaginaseisnotrelatedtothegrowthofbacteria.Whenthespecificgrowthrateofbacteriawascontrolledat0.15～0.30h-1,theQpmaxreached3922u/(gh).Keywords:pH;E.Coli;L-asparaginase;fermentativekinetics(编辑　李胜春)(109)ProcessofOxictoAffecttheSystemofDenitrifyingPhosphorusRemovalGAOXiao-ping,JIFang-ying,DINGCai-juan,ZUONing,ZHANGBin(KeyLaboratoryoftheDepartmentofEcologyEnvironmentEducationinThreeGorgesAreas,ChongqingUniversity,Chongqing400030,China)Abstract:Thephenomenonofdenitrifyingcanbefoundundertheconditionofanaerobic/anoxic(A/A)inthebiologicalphosphorusremova.lItisfoundthroughthetestthat:TheabilityofdenitrifyingphosphorusremovalinthesystemofA/A-SBRislowerthanthesystemofA/AO-SBR,andalongwiththetimeincreasinginthesystemofA/A-SBR,theabilityofphosphorusremovalwouldweaken,Yswillalsodecreasegraduallyfrominitial0.22gMLSS/gCOD.dt